WORKLIST ENTRIES (1):
5HT2BRECEPTR View alignment View Structure 5-hydroxytryptamine 2B receptor signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR01101 5HTRECEPTOR; PR00516 5HT2ARECEPTR; PR00517 5HT2CRECEPTR
INTERPRO; IPR000482
Creation date 07-APR-1997; UPDATE 06-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
5-Hydroxytryptamine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.159-180.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
5-Hydroxytryptamine (or serotonin) is ubiquitous in plants and animals.
It is an important neurotransmitter and local hormone in the CNS and
instestine, and is implicated in a vast array of physiological and patho-
physiological pathways [6]. In the periphery, 5HT contracts a number of
smooth muscles, and induces endothelium-dependent vasodilation through
the formation of NO [6]. It is a mediator of peristalsis, and may be
involved in platelet aggregation and homeostasis. In the CNS, 5HT is
believed to be involved in a wide range of functions, including the
control of appetite, mood, anxiety, hallucinations, sleep, vomiting and
pain perception [6]. 5HT receptor ligands are of clinical use in the
treatment of depression, migraine and post-operative vomiting.
Numerous receptor subtypes have been classified according to their
antagonist susceptibilities and their affinities for 5HT. Five 5HT1
subtypes and at least three 5HT2 subtypes have now been identified, in
addition to subtypes 5HT3-7 [6]. All share a high degree of sequence
similarity, and have overlapping pharmacological specificities.
The 5HT2 receptor was originally classified according to its ability to
display micromolar affinity for 5HT, to be labelled with [3H]spiperone,
and by its susceptibility to 5HT antagonists [6]. At least 3 members of
the family exist (including the re-classified 5HT1C receptor), all of
which share a high degree of sequence similarity and stimulate the
phosphoinositide pathway.
5HT2BRECEPTR is an 8-element fingerprint that provides a signature for the
5HT2B receptors. The fingerprint was derived from an initial alignment of
4 sequences: the motifs were drawn from conserved sections within either
loop or N- and C-terminal regions, focusing on those areas of the alignment
that characterise the 5HT2B receptors but distinguish them from the rest of
the 5HT family - motif 1 lies at the N-terminus; motif 2 spans the second
cytoplasmic loop; motifs 3-5 span the third cytoplasmic loop; and motifs
6-8 lie at the C-terminus. A single iteration on OWL29.1 was required to
reach convergence, no further sequences being identified beyond the starting
set. A single partial match was found, CPU69635, a 5HT2B receptor fragment
that matches motifs 6 and 7.
An update on SPTR37_9f identified a true set of 3 sequences.
SUMMARY INFORMATION
3 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 3 3 3 3 3 3 3 3
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
5H2B_MOUSE 5H2B_RAT 5H2B_HUMAN
PROTEIN TITLES
5H2B_MOUSE 5-HYDROXYTRYPTAMINE 2B RECEPTOR (5-HT-2B) (SEROTONIN RECEPTO
5H2B_RAT 5-HYDROXYTRYPTAMINE 2B RECEPTOR (5-HT-2B) (SEROTONIN RECEPTO
5H2B_HUMAN 5-HYDROXYTRYPTAMINE 2B RECEPTOR (5-HT-2B) (SEROTONIN RECEPTO
SCAN HISTORY
OWL29_1 1 100 NSINGLE
SPTR37_9f 2 4 NSINGLE
INITIAL MOTIF SETS
5HT2BRECEPTR1 Length of motif = 21 Motif number = 1
5H2B receptor motif I - 1
PCODE ST INT
HLILTNRSGLETDSVAEEMKQ 5H2B_MOUSE 24 24
HLILTDRSGLKAESAAEEMKQ 5H2B_RAT 24 24
HVISSNWSGLQTESIPEEMKQ 5H2B_HUMAN 25 25
HVISSNWSGLQTESIPEEMKQ S49442 25 25
5HT2BRECEPTR2 Length of motif = 15 Motif number = 2
5H2B receptor motif II - 1
PCODE ST INT
IQANQCNTRATAFIK 5H2B_MOUSE 160 115
IQANQCNSRTTAFVK 5H2B_RAT 160 115
IQANQYNSRATAFIK 5H2B_HUMAN 161 115
IQANQYNSRATAFIK S49442 161 115
5HT2BRECEPTR3 Length of motif = 19 Motif number = 3
5H2B receptor motif III - 1
PCODE ST INT
HTLQKKAYLVKNKPPQRLT 5H2B_MOUSE 241 66
HALRKKAYLVRNRPPQRLT 5H2B_RAT 241 66
HALQKKAYLVKNKPPQRLT 5H2B_HUMAN 242 66
HALQKKAYLVKNKPPQRLT S49442 242 66
5HT2BRECEPTR4 Length of motif = 21 Motif number = 4
5H2B receptor motif IV - 1
PCODE ST INT
REDSSFSSPEKVAMLDGSHRD 5H2B_MOUSE 269 9
REDSSFSSPEKMVMLDGSHKD 5H2B_RAT 269 9
RDETPCSSPEKVAMLDGSRKD 5H2B_HUMAN 270 9
RDETPCSSPEKVAMLDGSRKD S49442 270 9
5HT2BRECEPTR5 Length of motif = 20 Motif number = 5
5H2B receptor motif V - 1
PCODE ST INT
KILPNSSDETLMRRMSSVGK 5H2B_MOUSE 290 0
KILPNSTDETLMRRMSSAGK 5H2B_RAT 290 0
KALPNSGDETLMRRTSTIGK 5H2B_HUMAN 291 0
KALPNSGDETLMRRTSTIGK S49442 291 0
5HT2BRECEPTR6 Length of motif = 17 Motif number = 6
5H2B receptor motif VI - 1
PCODE ST INT
ATKSVKALRKFSSTLCF 5H2B_MOUSE 400 90
ATKSVKVLRKCSSTLYF 5H2B_RAT 400 90
ATKSVKTLRKRSSKIYF 5H2B_HUMAN 401 90
ATKSVKTLRKRSSKIYF S49442 401 90
5HT2BRECEPTR7 Length of motif = 22 Motif number = 7
5H2B receptor motif VII - 1
PCODE ST INT
MVENSKFFTKHGIRNGINPAMY 5H2B_MOUSE 420 3
MVENSKFFTKHGIRNGINPAMY 5H2B_RAT 420 3
MAENSKFFKKHGIRNGINPAMY 5H2B_HUMAN 421 3
MAENSKFFKKHGIRNGINPAMY S49442 421 3
5HT2BRECEPTR8 Length of motif = 23 Motif number = 8
5H2B receptor motif VIII - 1
PCODE ST INT
QSPMRLRCSTIQSSSIILLDTLL 5H2B_MOUSE 442 0
QSPVRLRSSTIQSSSIILLNTFL 5H2B_RAT 442 0
QSPMRLRSSTIQSSSIILLDTLL 5H2B_HUMAN 443 0
QSPMRLRSSPIQSSSIILLDTLL S49442 443 0
FINAL MOTIF SETS
5HT2BRECEPTR1 Length of motif = 21 Motif number = 1
5H2B receptor motif I - 2
PCODE ST INT
HLILTNRSGLETDSVAEEMKQ 5H2B_MOUSE 24 24
HLILTDRSGLKAESAAEEMKQ 5H2B_RAT 24 24
HVISSNWSGLQTESIPEEMKQ 5H2B_HUMAN 25 25
5HT2BRECEPTR2 Length of motif = 15 Motif number = 2
5H2B receptor motif II - 2
PCODE ST INT
IQANQCNTRATAFIK 5H2B_MOUSE 160 115
IQANQCNSRTTAFVK 5H2B_RAT 160 115
IQANQYNSRATAFIK 5H2B_HUMAN 161 115
5HT2BRECEPTR3 Length of motif = 19 Motif number = 3
5H2B receptor motif III - 2
PCODE ST INT
HTLQKKAYLVKNKPPQRLT 5H2B_MOUSE 241 66
HALRKKAYLVRNRPPQRLT 5H2B_RAT 241 66
HALQKKAYLVKNKPPQRLT 5H2B_HUMAN 242 66
5HT2BRECEPTR4 Length of motif = 21 Motif number = 4
5H2B receptor motif IV - 2
PCODE ST INT
REDSSFSSPEKVAMLDGSHRD 5H2B_MOUSE 269 9
REDSSFSSPEKMVMLDGSHKD 5H2B_RAT 269 9
RDETPCSSPEKVAMLDGSRKD 5H2B_HUMAN 270 9
5HT2BRECEPTR5 Length of motif = 20 Motif number = 5
5H2B receptor motif V - 2
PCODE ST INT
KILPNSSDETLMRRMSSVGK 5H2B_MOUSE 290 0
KILPNSTDETLMRRMSSAGK 5H2B_RAT 290 0
KALPNSGDETLMRRTSTIGK 5H2B_HUMAN 291 0
5HT2BRECEPTR6 Length of motif = 17 Motif number = 6
5H2B receptor motif VI - 2
PCODE ST INT
ATKSVKALRKFSSTLCF 5H2B_MOUSE 400 90
ATKSVKVLRKCSSTLYF 5H2B_RAT 400 90
ATKSVKTLRKRSSKIYF 5H2B_HUMAN 401 90
5HT2BRECEPTR7 Length of motif = 22 Motif number = 7
5H2B receptor motif VII - 2
PCODE ST INT
MVENSKFFTKHGIRNGINPAMY 5H2B_MOUSE 420 3
MVENSKFFTKHGIRNGINPAMY 5H2B_RAT 420 3
MAENSKFFKKHGIRNGINPAMY 5H2B_HUMAN 421 3
5HT2BRECEPTR8 Length of motif = 23 Motif number = 8
5H2B receptor motif VIII - 2
PCODE ST INT
QSPMRLRCSTIQSSSIILLDTLL 5H2B_MOUSE 442 0
QSPVRLRSSTIQSSSIILLNTFL 5H2B_RAT 442 0
QSPMRLRSSTIQSSSIILLDTLL 5H2B_HUMAN 443 0
User query: Display/Full Code "5HT2BRECEPTR"