WORKLIST ENTRIES (1):
5HT2CRECEPTR View alignment View Structure 5-hydroxytryptamine 2C receptor signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR01101 5HTRECEPTOR; PR00516 5HT2ARECEPTR; PR00651 5HT2BRECEPTR
INTERPRO; IPR000377
Creation date 04-JUN-1996; UPDATE 06-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
5-Hydroxytryptamine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.159-180.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
5-Hydroxytryptamine (or serotonin) is ubiquitous in plants and animals.
It is an important neurotransmitter and local hormone in the CNS and
instestine, and is implicated in a vast array of physiological and patho-
physiological pathways [6]. In the periphery, 5HT contracts a number of
smooth muscles, and induces endothelium-dependent vasodilation through
the formation of NO [6]. It is a mediator of peristalsis, and may be
involved in platelet aggregation and homeostasis. In the CNS, 5HT is
believed to be involved in a wide range of functions, including the
control of appetite, mood, anxiety, hallucinations, sleep, vomiting and
pain perception [6]. 5HT receptor ligands are of clinical use in the
treatment of depression, migraine and post-operative vomiting.
Numerous receptor subtypes have been classified according to their
antagonist susceptibilities and their affinities for 5HT. Five 5HT1
subtypes and at least three 5HT2 subtypes have now been identified, in
addition to subtypes 5HT3-7 [6]. All share a high degree of sequence
similarity, and have overlapping pharmacological specificities.
The 5HT2 receptor was originally classified according to its ability to
display micromolar affinity for 5HT, to be labelled with [3H]spiperone,
and by its susceptibility to 5HT antagonists [6]. At least 3 members of
the family exist (including the re-classified 5HT1C receptor), all of
which share a high degree of sequence similarity and stimulate the
phosphoinositide pathway.
5HT2CRECEPTR is an 8-element fingerprint that provides a signature for the
5HT2C receptors. The fingerprint was derived from an initial alignment of
3 sequences: the motifs were drawn from conserved sections within either
loop or N- and C-terminal regions, focusing on those areas of the alignment
that characterise the 5HT2C receptors but distinguish them from the rest of
the 5HT family - motifs 1 and 2 lie at the N-terminus; motifs 3 and 4 span
the third cytoplasmic loop; and motifs 6-8 lie at the C-terminus. A single
iteration on OWL28.0 was required to reach convergence, no further sequences
being identified beyond the starting set. A single partial match was also
found (HS5HT2C), a 5HT2C receptor fragment that matches motifs 1 and 2.
An update on SPTR37_9f identified a true set of 3 sequences, and 2
partial matches.
SUMMARY INFORMATION
3 codes involving 8 elements
0 codes involving 7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 3 3 3 3 3 3 3 3
7| 0 0 0 0 0 0 0 0
6| 0 0 1 1 1 1 1 1
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 1 1 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
5H2C_MOUSE 5H2C_RAT 5H2C_HUMAN
Subfamily: Codes involving 6 elements
Subfamily True positives..
Q62842
Subfamily: Codes involving 2 elements
Subfamily True positives..
Q13638
PROTEIN TITLES
5H2C_MOUSE 5-HYDROXYTRYPTAMINE 2C RECEPTOR (5-HT-2C) (SEROTONIN RECEPTO
5H2C_RAT 5-HYDROXYTRYPTAMINE 2C RECEPTOR (5-HT-2C) (SEROTONIN RECEPTO
5H2C_HUMAN 5-HYDROXYTRYPTAMINE 2C RECEPTOR (5-HT-2C) (SEROTONIN RECEPTO
Q62842 SEROTONIN 5-HT2C - RATTUS NORVEGICUS (RAT).
Q13638 5-HT2C RECEPTOR - HOMO SAPIENS (HUMAN).
SCAN HISTORY
OWL28_0 1 50 NSINGLE
SPTR37_9f 2 6 NSINGLE
INITIAL MOTIF SETS
5HT2CRECEPTR1 Length of motif = 20 Motif number = 1
5HT2C receptor motif I - 1
PCODE ST INT
AVRSLLVHLIGLLVWQFDIS 5H2C_MOUSE 7 7
AVRSLLMHLIGLLVWQFDIS 5H2C_RAT 7 7
AVHSFLVHLIGLLVWQCDIS 5H2C_HUMAN 7 7
5HT2CRECEPTR2 Length of motif = 18 Motif number = 2
5HT2C receptor motif II - 1
PCODE ST INT
SPVAAIVTDTFNSSDGGR 5H2C_MOUSE 28 1
SPVAAIVTDTFNSSDGGR 5H2C_RAT 28 1
SPVAAIVTDIFNTSDGGR 5H2C_HUMAN 28 1
5HT2CRECEPTR3 Length of motif = 13 Motif number = 3
5HT2C receptor motif III - 1
PCODE ST INT
EEENAPNPNPDQK 5H2C_MOUSE 275 229
EEENAPNPNPDQK 5H2C_RAT 276 230
EEENSANPNQDQN 5H2C_HUMAN 273 227
5HT2CRECEPTR4 Length of motif = 12 Motif number = 4
5HT2C receptor motif IV - 1
PCODE ST INT
RRKKKEKRPRGT 5H2C_MOUSE 289 1
RRKKKEKRPRGT 5H2C_RAT 290 1
RRKKKERRPRGT 5H2C_HUMAN 288 2
5HT2CRECEPTR5 Length of motif = 17 Motif number = 5
5HT2C receptor motif V - 1
PCODE ST INT
PPVRQIPRVAATALSGR 5H2C_MOUSE 394 93
PPVRQIPRVAATALSGR 5H2C_RAT 395 93
PPVRQIPRVAATALSGR 5H2C_HUMAN 393 93
5HT2CRECEPTR6 Length of motif = 17 Motif number = 6
5HT2C receptor motif VI - 1
PCODE ST INT
ELNVNIYRHTNERVVRK 5H2C_MOUSE 411 0
ELNVNIYRHTNERVARK 5H2C_RAT 412 0
ELNVNIYRHTNEPVIEK 5H2C_HUMAN 410 0
5HT2CRECEPTR7 Length of motif = 16 Motif number = 7
5HT2C receptor motif VII - 1
PCODE ST INT
ANDTEPGIEMQVENLE 5H2C_MOUSE 428 0
ANDPEPGIEMQVENLE 5H2C_RAT 429 0
ASDNEPGIEMQVENLE 5H2C_HUMAN 427 0
5HT2CRECEPTR8 Length of motif = 15 Motif number = 8
5HT2C receptor motif VIII - 1
PCODE ST INT
ELPVNPSNVVSERIS 5H2C_MOUSE 443 -1
ELPVNPSNVVSERIS 5H2C_RAT 444 -1
ELPVNPSSVVSERIS 5H2C_HUMAN 442 -1
FINAL MOTIF SETS
5HT2CRECEPTR1 Length of motif = 20 Motif number = 1
5HT2C receptor motif I - 2
PCODE ST INT
AVRSLLVHLIGLLVWQFDIS 5H2C_MOUSE 7 7
AVRSLLMHLIGLLVWQFDIS 5H2C_RAT 7 7
AVHSFLVHLIGLLVWQCDIS 5H2C_HUMAN 7 7
5HT2CRECEPTR2 Length of motif = 18 Motif number = 2
5HT2C receptor motif II - 2
PCODE ST INT
SPVAAIVTDTFNSSDGGR 5H2C_MOUSE 28 1
SPVAAIVTDTFNSSDGGR 5H2C_RAT 28 1
SPVAAIVTDIFNTSDGGR 5H2C_HUMAN 28 1
5HT2CRECEPTR3 Length of motif = 13 Motif number = 3
5HT2C receptor motif III - 2
PCODE ST INT
EEENAPNPNPDQK 5H2C_MOUSE 275 229
EEENAPNPNPDQK 5H2C_RAT 276 230
EEENSANPNQDQN 5H2C_HUMAN 273 227
5HT2CRECEPTR4 Length of motif = 12 Motif number = 4
5HT2C receptor motif IV - 2
PCODE ST INT
RRKKKEKRPRGT 5H2C_MOUSE 289 1
RRKKKEKRPRGT 5H2C_RAT 290 1
RRKKKERRPRGT 5H2C_HUMAN 288 2
5HT2CRECEPTR5 Length of motif = 17 Motif number = 5
5HT2C receptor motif V - 2
PCODE ST INT
PPVRQIPRVAATALSGR 5H2C_MOUSE 394 93
PPVRQIPRVAATALSGR 5H2C_RAT 395 93
PPVRQIPRVAATALSGR 5H2C_HUMAN 393 93
5HT2CRECEPTR6 Length of motif = 17 Motif number = 6
5HT2C receptor motif VI - 2
PCODE ST INT
ELNVNIYRHTNERVVRK 5H2C_MOUSE 411 0
ELNVNIYRHTNERVARK 5H2C_RAT 412 0
ELNVNIYRHTNEPVIEK 5H2C_HUMAN 410 0
5HT2CRECEPTR7 Length of motif = 16 Motif number = 7
5HT2C receptor motif VII - 2
PCODE ST INT
ANDTEPGIEMQVENLE 5H2C_MOUSE 428 0
ANDPEPGIEMQVENLE 5H2C_RAT 429 0
ASDNEPGIEMQVENLE 5H2C_HUMAN 427 0
5HT2CRECEPTR8 Length of motif = 15 Motif number = 8
5HT2C receptor motif VIII - 2
PCODE ST INT
ELPVNPSNVVSERIS 5H2C_MOUSE 443 -1
ELPVNPSNVVSERIS 5H2C_RAT 444 -1
ELPVNPSSVVSERIS 5H2C_HUMAN 442 -1
User query: Display/Full Code "5HT2CRECEPTR"