WORKLIST ENTRIES (1):
ADRENRGCA1AR View alignment View Structure Alpha-1A adrenergic receptor signature
Type of fingerprint: COMPOUND with 6 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR01103 ADRENERGICR; PR00240 ADRENRGCA1DR; PR00556 ADRENRGCA1BR
INTERPRO; IPR001004
Creation date 14-AUG-1996; UPDATE 18-FEB-2000
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Adrenaline and noradrenaline.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.32-54.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
In the periphery, the adrenergic system plays an important role in
regulating the cardiovascular system [6]. Increased sympathetic discharge
to the heart increases the rate and force of contraction mediated through
beta-1 receptors. Circulating adrenaline also acts on cardiac tissue, and,
in addition acts both on alpha-1 adrenoceptors in arterial smooth muscle,
stimulating vasoconstriction, and on beta-2 adrenoceptors in vascular beds
of skeletal muscle, stimulating vasodilation [6]. In the CNS, noradrenaline
is thought to be involved in the regulation of mood, and various psycho-
active drugs alter noradrenergic function. Numerous drugs exert their
actions via adrenoceptors: e.g., beta-2 selective agonists such as
salbutamol are used in the acute treatment of asthma, while alpha agonists
prolong the action of local anaesthetics, and act as nasal decongestants [6].
Adrenoceptors can be divided into three main classes based on sequence
similarity, receptor pharmacology and signalling mechanisms. Further
subdivisions exist within each class [6]. A large number of agonists and
antagonists distinguish between the different classes of adrenoceptor; by
contrast, relatively small differences in agonist and antagonist affinities
are demonstrated, especially within the alpha-1 and alpha-2 adrenoceptor
subtypes [6].
The alpha-1A receptor has not been detected in rat or bovine tissues by
Northern analysis [6]. Its expression is thus either very low, or highly
specialised in tissue distribution [6]. The receptor is coupled to the
phosphoinositide pathway through a pertussis-toxin-insensitive G protein,
probably of the Gq/G11 class [6].
ADRENRGCA1AR is a 6-element fingerprint that provides a signature for the
alpha-1A adrenergic receptors. The fingerprint was derived from an initial
alignment of 8 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the alpha-1A adrenergic receptors but
distinguish them from the rest of the rhodopsin-like superfamily - motif
1 lies at the N-terminus; motifs 2 and 3 lie in the third cytoplasmic loop;
and motifs 4-6 span the C-terminus. A single iteration on OWL28.1 was
required to reach convergence, no further sequences being identified beyond
the starting set. A single partial match was also found, an alpha-1C
receptor fragment that matches motifs 2 and 3.
An update on SPTR37_9f identified a true set of 8 sequences.
SUMMARY INFORMATION
8 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
6| 8 8 8 8 8 8
5| 0 0 0 0 0 0
4| 0 0 0 0 0 0
3| 0 0 0 0 0 0
2| 0 0 0 0 0 0
--+-------------------------------
| 1 2 3 4 5 6
True positives..
A1AA_HUMAN Q13729 Q13675 O60451
A1AA_RABIT A1AA_RAT O54913 A1AA_BOVIN
PROTEIN TITLES
A1AA_HUMAN ALPHA-1A ADRENERGIC RECEPTOR (ALPHA 1A-ADRENOCEPTOR) (ALPHA-
Q13729 ALPHA 1C ADRENERGIC RECEPTOR ISOFORM 3 - HOMO SAPIENS (HUMAN
Q13675 ALPHA 1C ADRENERGIC RECEPTOR ISOFORM 2 - HOMO SAPIENS (HUMAN
O60451 ALPHA 1A ADRENERGIC RECEPTOR ISOFORM 4 - HOMO SAPIENS (HUMAN
A1AA_RABIT ALPHA-1A ADRENERGIC RECEPTOR (ALPHA 1A-ADRENOCEPTOR) (ALPHA-
A1AA_RAT ALPHA-1A ADRENERGIC RECEPTOR (ALPHA 1A-ADRENOCEPTOR) (ALPHA-
O54913 ALPHA 1A-ADRENERGIC RECEPTOR - MUS MUSCULUS (MOUSE).
A1AA_BOVIN ALPHA-1A ADRENERGIC RECEPTOR (ALPHA 1A-ADRENOCEPTOR) (ALPHA-
SCAN HISTORY
OWL28_1 1 50 NSINGLE
SPTR37_9f 2 9 NSINGLE
INITIAL MOTIF SETS
ADRENRGCA1AR1 Length of motif = 23 Motif number = 1
A1A adrenergic receptor motif I - 1
PCODE ST INT
FLSGNASDSSNCTQPPAPVNISK A1AC_HUMAN 3 3
FLSGNASDSSNCTQPPAPVNISK JN0765 3 3
FLSGNASDSSNCTQPPAPVNISK JC2333 3 3
FLSGNASDSSNCTQPPAPVNISK HSU02569 3 3
FLSGNASDSSNCTQPPAPVNISK HUMA1CAR2 3 3
FLSGNASDSSNCTHPPPPVNISK A1AC_BOVIN 3 3
LLSENASEGSNCTHPPAPVNISK A1AC_RAT 3 3
LLSENASEGSNCTHPPAPVNISK RNU13368 3 3
ADRENRGCA1AR2 Length of motif = 16 Motif number = 2
A1A adrenergic receptor motif II - 1
PCODE ST INT
KSGLKTDKSDSEQVTL A1AC_HUMAN 219 193
KSGLKTDKSDSEQVTL JN0765 219 193
KSGLKTDKSDSEQVTL JC2333 219 193
KSGLKTDKSDSEQVTL HSU02569 219 193
KSGLKTDKSDSEQVTL HUMA1CAR2 219 193
KSGLKTDKSDSEQVTL A1AC_BOVIN 219 193
KSGLKTDKSDSEQVTL A1AC_RAT 219 193
KSGLKTDKSDSEQVTL RNU13368 219 193
ADRENRGCA1AR3 Length of motif = 18 Motif number = 3
A1A adrenergic receptor motif III - 1
PCODE ST INT
RKNAPAGGSGMASAKTKT A1AC_HUMAN 238 3
RKNAPAGGSGMASAKTKT JN0765 238 3
RKNAPAGGSGHASAKTKT JC2333 238 3
RKNAPAGGSGMASAKTKT HSU02569 238 3
RKNAPAGGSGMASAKTKT HUMA1CAR2 238 3
RKNAQVGGSGVTSAKNKT A1AC_BOVIN 238 3
RKNVPAEGGGVSSAKNKT A1AC_RAT 238 3
RKNVPAEGGGVSSAKNKT RNU13368 238 3
ADRENRGCA1AR4 Length of motif = 18 Motif number = 4
A1A adrenergic receptor motif IV - 1
PCODE ST INT
LGYTLHPPSQAVEGQHKD A1AC_HUMAN 356 100
LGYTLHPPSQAVEGQHKD JN0765 356 100
LGYTLHPPSQAVEGQHKD JC2333 356 100
LGYPLHPPSQAVEGQHKD HSU02569 356 100
LGYTLHPPSQAVEGQHKD HUMA1CAR2 356 100
LGYTLHAPSHVLEGQHKD A1AC_BOVIN 356 100
LGYTLHPPSQALEGQHRD A1AC_RAT 356 100
LGYTLHPPSQALEGQHRD RNU13368 356 100
ADRENRGCA1AR5 Length of motif = 20 Motif number = 5
A1A adrenergic receptor motif V - 1
PCODE ST INT
RIPVGSRETFYRISKTDGVC A1AC_HUMAN 376 2
RIPVGSRETFYRISKTDGVC JN0765 376 2
RIPVGSRETFYRISKTDGVC JC2333 376 2
RIPVGSRETFYRISKTDGVC HSU02569 376 2
RIPVGSRETFYRISKTDGVC HUMA1CAR2 376 2
RIPVGSAETFYKISKTDGVC A1AC_BOVIN 376 2
RIPVGSGETFYKISKTDGVC A1AC_RAT 376 2
RIPVGSGETFYKISKTDGVC RNU13368 376 2
ADRENRGCA1AR6 Length of motif = 20 Motif number = 6
A1A adrenergic receptor motif VI - 1
PCODE ST INT
FSSMPRGSARITVSKDQSSC A1AC_HUMAN 400 4
FSSMPRGSARITVSKDQSSC JN0765 400 4
FSSMPRGSARITVSKDQSSC JC2333 400 4
FSSMPRGSARITVSKDQSSC HSU02569 400 4
FSSMPRGSARITVSKDQSSC HUMA1CAR2 400 4
FSSLPRGSARMAVARDPSAC A1AC_BOVIN 400 4
FSSMPQGSARITVPKDQSAC A1AC_RAT 400 4
FSSMPQGSARITVPKDQSAC RNU13368 400 4
FINAL MOTIF SETS
ADRENRGCA1AR1 Length of motif = 23 Motif number = 1
A1A adrenergic receptor motif I - 2
PCODE ST INT
FLSGNASDSSNCTQPPAPVNISK A1AA_HUMAN 3 3
FLSGNASDSSNCTQPPAPVNISK Q13729 3 3
FLSGNASDSSNCTQPPAPVNISK Q13675 3 3
FLSGNASDSSNCTQPPAPVNISK O60451 3 3
FLSGNASDSSNCTHPPAPVNISK A1AA_RABIT 3 3
LLSENASEGSNCTHPPAPVNISK A1AA_RAT 3 3
LLSENASEGSNCTHPPAQVNISK O54913 3 3
FLSGNASDSSNCTHPPPPVNISK A1AA_BOVIN 3 3
ADRENRGCA1AR2 Length of motif = 16 Motif number = 2
A1A adrenergic receptor motif II - 2
PCODE ST INT
KSGLKTDKSDSEQVTL A1AA_HUMAN 219 193
KSGLKTDKSDSEQVTL Q13729 219 193
KSGLKTDKSDSEQVTL Q13675 219 193
KSGLKTDKSDSEQVTL O60451 219 193
KSGLKTDKSDSEQVTL A1AA_RABIT 219 193
KSGLKTDKSDSEQVTL A1AA_RAT 219 193
KSGLKTDKSDSEQVTL O54913 219 193
KSGLKTDKSDSEQVTL A1AA_BOVIN 219 193
ADRENRGCA1AR3 Length of motif = 18 Motif number = 3
A1A adrenergic receptor motif III - 2
PCODE ST INT
RKNAPAGGSGMASAKTKT A1AA_HUMAN 238 3
RKNAPAGGSGMASAKTKT Q13729 238 3
RKNAPAGGSGMASAKTKT Q13675 238 3
RKNAPAGGSGMASAKTKT O60451 238 3
RKNAPAGGSGVASAKNKT A1AA_RABIT 238 3
RKNVPAEGGGVSSAKNKT A1AA_RAT 238 3
RKNVPAEGSGVSSAKNKT O54913 238 3
RKNAQVGGSGVTSAKNKT A1AA_BOVIN 238 3
ADRENRGCA1AR4 Length of motif = 18 Motif number = 4
A1A adrenergic receptor motif IV - 2
PCODE ST INT
LGYTLHPPSQAVEGQHKD A1AA_HUMAN 356 100
LGYTLHPPSQAVEGQHKD Q13729 356 100
LGYTLHPPSQAVEGQHKD Q13675 356 100
LGYTLHPPSQAVEGQHKD O60451 356 100
LGYTLHAPSQALEGQHKD A1AA_RABIT 356 100
LGYTLHPPSQALEGQHRD A1AA_RAT 356 100
LGYTLHPPSQAVEEQHRG O54913 356 100
LGYTLHAPSHVLEGQHKD A1AA_BOVIN 356 100
ADRENRGCA1AR5 Length of motif = 20 Motif number = 5
A1A adrenergic receptor motif V - 2
PCODE ST INT
RIPVGSRETFYRISKTDGVC A1AA_HUMAN 376 2
RIPVGSRETFYRISKTDGVC Q13729 376 2
RIPVGSRETFYRISKTDGVC Q13675 376 2
RIPVGSRETFYRISKTDGVC O60451 376 2
RIPVGSGETFYKISKTDGVC A1AA_RABIT 376 2
RIPVGSGETFYKISKTDGVC A1AA_RAT 376 2
RIPVGSGETFYKISKTDGVC O54913 376 2
RIPVGSAETFYKISKTDGVC A1AA_BOVIN 376 2
ADRENRGCA1AR6 Length of motif = 20 Motif number = 6
A1A adrenergic receptor motif VI - 2
PCODE ST INT
FSSMPRGSARITVSKDQSSC A1AA_HUMAN 400 4
FSSMPRGSARITVSKDQSSC Q13729 400 4
FSSMPRGSARITVSKDQSSC Q13675 400 4
FSSMPRGSARITVSKDQSSC O60451 400 4
FSSMPRGSARITVPKDQSAC A1AA_RABIT 400 4
FSSMPQGSARITVPKDQSAC A1AA_RAT 400 4
FSSMPQGSARITMPKDQSAC O54913 400 4
FSSLPRGSARMAVARDPSAC A1AA_BOVIN 400 4
User query: Display/Full Code "ADRENRGCA1AR"