WORKLIST ENTRIES (1):

CALPAIN View alignment View Structure         Calpain cysteine protease (C2) family signature
 Type of fingerprint: COMPOUND with 9  elements
Links:
   PRINTS; PR00705 PAPAIN; PR00966 NIAPOTYPTASE; PR00703 ADVENDOPTASE
   PRINTS; PR00797 STREPTOPAIN; PR00707 UBCTHYDRLASE; PR00776 HEMOGLOBNASE
   PRINTS; PR00706 PYROGLUPTASE; PR00864 PREPILNPTASE; PR00916 2CENDOPTASE
   PRINTS; PR00917 SRSVCYSPTASE
   INTERPRO; IPR001300
   PROSITE; PS00139 THIOL_PROTEASE_CYS; PS00639 THIOL_PROTEASE_HIS
   PROSITE; PS00640 THIOL_PROTEASE_ASN
   PFAM; PF00648 Cys_protease_2

 Creation date 08-MAR-1997; UPDATE 10-JUN-1999

   1. RAWLINGS, N.D. AND BARRETT, A.J.
   Families of cysteine peptidases.
   METHODS ENZYMOL. 244 461-486 (1994).

   2. HATA, A., OHNO, S., AKITA, Y. AND SUZUKI, K.
   Tandemly reiterated negative enhancer-like elements regulate transcription
   of a human gene for the large subunit of calcium-dependent protease.
   J.BIOL.CHEM. 264(11) 6404-6411 (1989).

   3. SORIMACHI, H., IMAJOH-OHMI, S., EMORI, Y., KAWASAKI, H., OHNO, S., 
   MINAMI, Y. AND SUZUKI, K.
   Molecular cloning of a novel mammalian calcium-dependent protease distinct
   from both m- and mu-types. Specific expression of the mRNA in skeletal
   muscle.
   J.BIOL.CHEM. 264(33) 20106-20111 (1989).

   Cysteine protease activity is dependent on an active dyad of cysteine and
   histidine, the order and spacing of these residues varying in the 20 or so
   known families. Families C1, C2 and C10 are loosely termed papain-like, and 
   nearly half of all cysteine proteases are found exclusively in viruses [1].
   
   Calpain is an intracellular protease involved in many important cellular
   functions that are regulated by calcium [2]. The protein is a complex of 2
   polypeptide chains (light and heavy), with three known forms in mammals 
   [1,3]: a highly calcium-sensitive (i.e., micro-molar range) form known as
   mu-calpain, mu-CANP or calpain I; a form sensitive to calcium in the
   milli-molar range, known as m-calpain, m-CANP or calpain II; and a third
   form, known as p94, which is found in skeletal muscle only [3]. 
   
   All three forms have identical light but different heavy chains [1,2].
   The heavy chain comprises four domains: domain 2 contains the catalytic
   region; domain 4 binds calcium and regulates activity [1]. Domain 2 shows
   low levels of sequence similarity to papain; although the catalytic His has
   not been located by biochemical means, it is likely that calpain and papain
   are related [1]. Domain 4 has four EF hand calcium-binding regions and is
   simmilar to sorcin and the Ca2+-binding region of calpain light chain [1]. 
   
   Calpain shows preferential cleavage for Tyr-|-XAA, Met-|-XAA and Arg-|-XAA
   with leucine or valine as the P2 residue. The product of the Drosophila
   gene sol has also been shown to be similar to calpain [1].
   
   CALPAIN is a 9-element fingerprint that provides a signature for the 
   heavy chain of the calpain cysteine protease (C2) family. The fingerprint
   was derived from an initial alignment of 11 sequences: the motifs were
   drawn from conserved regions spanning virtually the full alignment length -
   motifs 2 and 3 span the region encoded by PROSITE pattern THIOL_PROTEASE_CYS
   (PS00139), which contains the catalytic Cys. Two iterations on OWL30.0 were
   required to reach convergence, at which point a true set comprising 33 
   sequences was identified. Several partial matches were also found: S57195,
   CAN3_PIG, CAN2_RABIT, SSU23954 and CAN1_PIG are calpain fragments that lack
   portions of sequence bearing one or more motifs; SOL_DROME is a Drosophila
   optic lobe protein that matches motifs 1, 3, 4, 6 and 7.
  
   An update on SPTR37_9f identified a true set of 30 sequences, and 11
   partial matches.

  SUMMARY INFORMATION
     30 codes involving  9 elements
      0 codes involving  8 elements
      0 codes involving  7 elements
      1 codes involving  6 elements
      2 codes involving  5 elements
      1 codes involving  4 elements
      3 codes involving  3 elements
      4 codes involving  2 elements

   COMPOSITE FINGERPRINT INDEX
  
    9|  30   30   30   30   30   30   30   30   30  
    8|   0    0    0    0    0    0    0    0    0  
    7|   0    0    0    0    0    0    0    0    0  
    6|   1    0    1    1    1    1    1    0    0  
    5|   2    0    2    2    0    2    2    0    0  
    4|   0    0    0    1    1    0    1    0    1  
    3|   0    0    2    1    2    1    2    0    1  
    2|   0    0    2    1    0    3    2    0    0  
   --+----------------------------------------------
     |   1    2    3    4    5    6    7    8    9  

True positives..
 O08702         O70376         O70482         CAN3_MOUSE     
 CAN3_RAT       O54843         O46596         CAN2_RAT       
 O88977         O35350         CAN3_HUMAN     CAN2_HUMAN     
 CAN2_MOUSE     O88666         P97571         CAN1_HUMAN     
 CAN3_CHICK     CAN2_CHICK     O42133         Q64698         
 CANX_CHICK     O14815         CAN_DROME      YKR2_CAEEL     
 O08688         CAN_SCHMA      O15484         O35646         
 Q22036         O88501         
Subfamily:  Codes involving 6 elements
 Subfamily True positives..
 O75808         
Subfamily:  Codes involving 5 elements
 Subfamily True positives..
 O61346         SOL_DROME      
Subfamily:  Codes involving 4 elements
 Subfamily True positives..
 Q22143         
Subfamily:  Codes involving 3 elements
 Subfamily True positives..
 Q00204         O02259         O44903         
Subfamily:  Codes involving 2 elements
 Subfamily True positives..
 O02260         P91312         Q22386         O18165         


  PROTEIN TITLES
   O08702           CALPAIN LP82 - RATTUS NORVEGICUS (RAT).
   O70376           CALPAIN ISOFORM LP85 - RATTUS NORVEGICUS (RAT).
   O70482           MUSCLE TYPE CALPAIN P94 - RATTUS NORVEGICUS (RAT).
   CAN3_MOUSE       CALPAIN P94, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCI
   CAN3_RAT         CALPAIN P94, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCI
   O54843           CALPAIN 2 (80KDA M-CALPAIN SUBUNIT) - MUS MUSCULUS (MOUSE).
   O46596           SKELETAL MUSCLE SPECIFIC CALPAIN - SUS SCROFA (PIG).
   CAN2_RAT         CALPAIN 2, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCIUM
   O88977           CALPAIN LP82 - MUS MUSCULUS (MOUSE).
   O35350           CALPAIN 1 (MICROMOLAR CALCIUM ACTIVATED NEUTRAL PROTEASE LAR
   CAN3_HUMAN       CALPAIN P94, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCI
   CAN2_HUMAN       CALPAIN 2, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCIUM
   CAN2_MOUSE       CALPAIN 2, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCIUM
   O88666           CALPAIN I LARGE SUBUNIT - MUS MUSCULUS (MOUSE).
   P97571           MU-CALPAIN LARGE SUBUNIT (EC 3.4.22.17) - RATTUS NORVEGICUS 
   CAN1_HUMAN       CALPAIN 1, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCIUM
   CAN3_CHICK       CALPAIN P94, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCI
   CAN2_CHICK       CALPAIN 2, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCIUM
   O42133           MUCL (EC 3.4.22.17) - GALLUS GALLUS (CHICKEN).
   Q64698           CALPAIN, LARGE (CATALYTIC) SUBUNIT (EC 3.4.22.17) (CALCIUM-A
   CANX_CHICK       CALPAIN, LARGE [CATALYTIC] SUBUNIT (EC 3.4.22.17) (CALCIUM- 
   O14815           DIGESTIVE TRACT-SPECIFIC CALPAIN (EC 3.4.22.17) - HOMO SAPIE
   CAN_DROME        CALPAIN (EC 3.4.22.17) (CALCIUM-ACTIVATED NEUTRAL PROTEINASE
   YKR2_CAEEL       HYPOTHETICAL 70.9 KD PROTEIN C06G4.2 IN CHROMOSOME III - CAE
   O08688           CALPAIN 5 (CALPAIN-LIKE PROTEASE) - MUS MUSCULUS (MOUSE).
   CAN_SCHMA        CALPAIN (EC 3.4.22.17) (CALCIUM-ACTIVATED NEUTRAL PROTEINASE
   O15484           CALPAIN-LIKE PROTEASE - HOMO SAPIENS (HUMAN).
   O35646           CALPAIN 6 (CALPAIN-LIKE PROTEASE) - MUS MUSCULUS (MOUSE).
   Q22036           TRA-3 - CAENORHABDITIS ELEGANS.
   O88501           CALPAIN-LIKE PROTEASE - RATTUS NORVEGICUS (RAT).
 
   O75808           SMALL OPTIC LOBES HOMOLOG - HOMO SAPIENS (HUMAN).
 
   O61346           SMALL OPTIC LOBES - DROSOPHILA MELANOGASTER (FRUIT FLY).
   SOL_DROME        SMALL OPTIC LOBES PROTEIN - DROSOPHILA MELANOGASTER (FRUIT F
 
   Q22143           T04A8.16 PROTEIN - CAENORHABDITIS ELEGANS.
 
   Q00204           PUTATIVE CYSTEINE PROTEASE PALB - EMERICELLA NIDULANS (ASPER
   O02259           F44F1.1 PROTEIN - CAENORHABDITIS ELEGANS.
   O44903           W05G11.4 PROTEIN - CAENORHABDITIS ELEGANS.
 
   O02260           F44F1.3 PROTEIN - CAENORHABDITIS ELEGANS.
   P91312           SIMILAR TO CALCIUM-ACTIVATED NEUTRAL PROTEINASES - CAENORHAB
   Q22386           T11A5.6 PROTEIN - CAENORHABDITIS ELEGANS.
   O18165           W04A4.4 PROTEIN - CAENORHABDITIS ELEGANS.

SCAN HISTORY OWL30_0 2 40 NSINGLE SPTR37_9f 2 77 NSINGLE INITIAL MOTIF SETS CALPAIN1 Length of motif = 24 Motif number = 1 Calpain motif I - 1 PCODE ST INT QDYEALRNECLEAGTLFQDPSFPA CAN2_HUMAN 30 30 QDYETILNSCLASGSLFEDPLFPA CAN_DROME 73 73 QDYEQLRVRCLQSGTLFRDEAFPP CAN1_HUMAN 40 40 QNYSALRRDCRRRKVLFEDPLFPA HSNCL3PRT 11 11 QDFYELRDQCLESKRLFEDPQFLA YKR2_CAEEL 205 205 KTYEELHKKCLEENILYEDPDFPP CAN3_CHICK 53 53 KTFEQLHKKCLEKKVLYLDPEFPP CAN3_RAT 59 59 QKYQELKQECMKDGRLFCDPTFLP MMCALPAIN 11 11 QKYQELKQECIKDSRLFCDPTFLP AF029232 11 11 QKYQELKQECIKDSRLFCDPTFLP HSCANPX 11 11 KQYETLVKRLKTERTLWEDPDFPA CAN_SCHMA 84 84 CALPAIN2 Length of motif = 23 Motif number = 2 Calpain motif II - 1 PCODE ST INT WKRPTEICADPQFIIGGATRTDI CAN2_HUMAN 75 21 WLRPHEIAENPQFFVEGYSRFDV CAN_DROME 113 16 WKRPTELLSNPQFIVDGATRTDI CAN1_HUMAN 85 21 RKRPKGICEDPRLFVDGISSHDL HSNCL3PRT 51 16 WLRPGEITREPQLITEGHSRFDV YKR2_CAEEL 245 16 WKRPREICENPRFIIGGANRTDI CAN3_CHICK 93 16 WKRPPEICENPRFIIGGANRTDI CAN3_RAT 99 16 WKRPQDISDDPHLIVGNISNHQL MMCALPAIN 51 16 WKRPQDICDDPHLIVGNISNHQL AF029232 51 16 WKRPQDICDDPHLIVGNISNHQL HSCANPX 51 16 WKRPHEINPNAKFFAGGASRFDI CAN_SCHMA 124 16 CALPAIN3 Length of motif = 17 Motif number = 3 Calpain motif III - 1 PCODE ST INT QGALGDCWLLAAIASLT CAN2_HUMAN 99 1 QGELGDCWLLAATANLT CAN_DROME 137 1 QGALGDCWLLAAIASLT CAN1_HUMAN 109 1 QGQVGNCWFVAACSSLA HSNCL3PRT 75 1 QGELGDCWLLAAAANLT YKR2_CAEEL 269 1 QGELGDCWFLAAIACLT CAN3_CHICK 117 1 QGDLGDCWLLAAIACLT CAN3_RAT 123 1 QGRLGNKAMISAFSCLA MMCALPAIN 75 1 QGRLGHKPMVSAFSCLA AF029232 75 1 QGRLGHKPMVSAFSCLA HSCANPX 75 1 QGALGDCWLLAVVASIS CAN_SCHMA 148 1 CALPAIN4 Length of motif = 26 Motif number = 4 Calpain motif IV - 1 PCODE ST INT YAGIFHFQFWQYGEWVEVVVDDRLPT CAN2_HUMAN 135 19 YAGIFHFRFWQYGKWVDVIIDDRLPT CAN_DROME 173 19 YAGIFHFQLWQFGEWVDVVVDDLLPI CAN1_HUMAN 145 19 YAGIFHFHFWRFGEWVDVVIDDRLPT HSNCL3PRT 117 25 YAGIFHFQFWQYGKWVDVVIDDRLPT YKR2_CAEEL 305 19 YAGIFHFQFWRYGDWVDVIIDDCLPT CAN3_CHICK 153 19 YAGIFHFQFWRYGDWVDVVIDDCLPT CAN3_RAT 159 19 YAGIFHFRFWHFGEWTEVVIDDLLPT MMCALPAIN 117 25 YAGIFHFRFWHFGEWTEVVIDDLLPT AF029232 117 25 YAGIFHFRFWHFGEWTEVVIDDLLPT HSCANPX 117 25 YVGVVRFRFWRFGHWVEVLIDDRLPV CAN_SCHMA 185 20 CALPAIN5 Length of motif = 24 Motif number = 5 Calpain motif V - 1 PCODE ST INT LLFVHSAEGSEFWSALLEKAYAKI CAN2_HUMAN 165 4 LMYMHSTEKNEFWSALLEKAYAKL CAN_DROME 203 4 LVFVHSAEGNEFWSALLEKAYAKV CAN1_HUMAN 175 4 LIYCHSNSRNEFWCALVEKAYAKL HSNCL3PRT 147 4 LLYMHSASNNEFWSALLEKAYAKL YKR2_CAEEL 335 4 LVFTKSSQRNEFWSALLEKAYAKL CAN3_CHICK 183 4 LVFTKSNHRNEFWSALLEKAYAKL CAN3_RAT 189 4 LVFSFSTSMNEFWNALLEKAYAKL MMCALPAIN 147 4 LVFSFSTSMNEFWNALLEKAYAKL AF029232 147 4 LVFSFSTSMNEFWNALLEKAYAKL HSCANPX 147 4 LVFMHSNDPTEFWSALLEKAYAKL CAN_SCHMA 217 6 CALPAIN6 Length of motif = 28 Motif number = 6 Calpain motif VI - 1 PCODE ST INT GCYEALSGGATTEGFEDFTGGIAEWYEL CAN2_HUMAN 190 1 GSYEALKGGSTCEAMEDFTGGVSEWYDL CAN_DROME 228 1 GSYEALSGGSTSEGFEDFTGGVTEWYEL CAN1_HUMAN 200 1 GCYQALDGGNTADALVDFTGGVSEPIDL HSNCL3PRT 172 1 GSYEALKGGTTSEALEDMTGGLTEFIDL YKR2_CAEEL 360 1 GSYEALKGGNTTEAMEDFTGGVIEFYEI CAN3_CHICK 208 1 GSYEALKGGNTTEAMEDFTGGVTEFFEI CAN3_RAT 214 1 GCYEALDGLTITDIIMDFTGTLAEIIDM MMCALPAIN 172 1 GCYEALDGLTITDIIVDFTGTLAETVDM AF029232 172 1 GCYEALDGLTITDIIVDFTGTLAETVDM HSCANPX 172 1 GCYAHLSGGSQSEAMEDLTGGICLSLEL CAN_SCHMA 242 1 CALPAIN7 Length of motif = 22 Motif number = 7 Calpain motif VII - 1 PCODE ST INT EDGEFWMSFSDFLRHYSRLEIC CAN2_HUMAN 320 102 RDGEFWMSFQDFLNHFDRVEIC CAN_DROME 363 107 EDGEFWMSFRDFMREFTRLEIC CAN1_HUMAN 330 102 DDGEFWMTFEDVCRYFTDIIKC HSNCL3PRT 319 119 HDGEFWMSFDDFMRNFEKMEIC YKR2_CAEEL 491 103 EDGEFWISLEDFMRHFTKLEIC CAN3_CHICK 386 150 EDGEFWMSYDDFVYHFTKLEIC CAN3_RAT 393 151 DDGEFWMSLEDFCHNFHKLNVC MMCALPAIN 319 119 DDGEFWMSLEDFCRNFHKLNVC AF029232 319 119 DDGEFWMSLEDFCRNFHKLNVC HSCANPX 319 119 ADGEFWMSYEDFVTCFSRVEVC CAN_SCHMA 373 103 CALPAIN8 Length of motif = 18 Motif number = 8 Calpain motif VIII - 1 PCODE ST INT TAGGCRNYPNTFWMNPQY CAN2_HUMAN 370 28 TAGGCRNFLDTFWHNPQY CAN_DROME 417 32 TAGGCRNYPATFWVNPQF CAN1_HUMAN 380 28 RGGGCINHKDTFFQNPQY HSNCL3PRT 373 32 TAGGCRNYINTFANNPQF YKR2_CAEEL 549 36 SAGGCRNYPDTFWTNPQY CAN3_CHICK 436 28 SAGGCRNFPDTFWTNPQY CAN3_RAT 443 28 RSGGCYNNRDTFLQNPQY MMCALPAIN 370 29 RSGGCYNNRDTFLQNPQY AF029232 370 29 RSGGCYNNRDTFLQNPQY HSCANPX 370 29 NAGGCINNRTTYWTSPQF CAN_SCHMA 427 32 CALPAIN9 Length of motif = 29 Motif number = 9 Calpain motif IX - 1 PCODE ST INT KLPPGEYILVPSTFEPNKDGDFCIRVFSE CAN2_HUMAN 476 88 KLPPGHYLIVPSTFDPNEEGEFIIRVFSE CAN_DROME 519 84 RLPPGEYVVVPSTFEPNKEGDFVLRFFSE CAN1_HUMAN 488 90 DQPEGRYVIIPTTFEPGHTGEFLLRVFTD HSNCL3PRT 462 71 RVPPGNYVVVPSTFEPNEEAEFMLRVYTN YKR2_CAEEL 619 52 RLPPSEYVIIPSTYEPHQEGEFILRVFSE CAN3_CHICK 541 87 RLPPSEYVIVPSTYEPHQEGEFILRVFSE CAN3_RAT 548 87 YLKKGSYVLVPTMFQHGRTSEFLLRIFSE MMCALPAIN 461 73 YLKKGNYVLVPTMFQHGRTSEFLLRIFSE AF029232 461 73 YLKKGNYVLVPTMFQHGRTSEFLLRIFSE HSCANPX 461 73 RVPPGSYVVIPSTFDPNIEVNFILRVFSQ CAN_SCHMA 524 79 FINAL MOTIF SETS CALPAIN1 Length of motif = 24 Motif number = 1 Calpain motif I - 2 PCODE ST INT QDFVVLKQRCLAQKCLFEDRVFPA O08702 35 35 QDFVVLKQRCLAQKCLFEDRVFPA O70376 35 35 QDFVVLKQRCLAQKCLFEDRVFPA O70482 35 35 KTFEQLRKKCLEKKVLYLDPEFPP CAN3_MOUSE 59 59 KTFEQLHKKCLEKKVLYLDPEFPP CAN3_RAT 59 59 QDYETLRNECLEAGALFQDPSFPA O54843 30 30 KTFEQLHKKCLEKKVLYLDPEFPP O46596 58 58 QDYETLRNECLEAGALFQDPSFPA CAN2_RAT 30 30 QDFVVLKQRCLAQKCLFEDRVFPA O88977 35 35 QDYETLRARCLQSGVLFQDEAFPP O35350 40 40 KTFEQLHKKCLEKKVLYVDPEFPP CAN3_HUMAN 59 59 QDYEALRNECLEAGTLFQDPSFPA CAN2_HUMAN 30 30 QDYETLRNECLEAGALFQDPSFPA CAN2_MOUSE 30 30 QDYETLRARCLQSGVLFQDEAFPP O88666 40 40 QDYENLRARCLQNGVLFQDDAFPP P97571 40 40 QDYEQLRVRCLQSGTLFRDEAFPP CAN1_HUMAN 40 40 KTYEELHKKCLEENILYEDPDFPP CAN3_CHICK 53 53 QDFGALRRECLQGGRLFHDPSFPA CAN2_CHICK 30 30 QDYAALRDDCLRSGSLFRDETFPP O42133 40 40 QDFETLRKQCLNSGVLFKDPEFPA Q64698 30 30 QDYEALKQECIESGTLFRDPQFPA CANX_CHICK 32 32 QSFEQMRQECLQRGTLFEDADFPA O14815 27 27 QDYETILNSCLASGSLFEDPLFPA CAN_DROME 73 73 QDFYELRDQCLESKRLFEDPQFLA YKR2_CAEEL 205 205 QNYSALKRACLRKKVLFEDPLFPA O08688 11 11 KQYETLVKRLKTERTLWEDPDFPA CAN_SCHMA 84 84 QNYSALRQDCRRRKVLFEDPLFPA O15484 11 11 QKYQELKQECMKDGRLFCDPTFLP O35646 11 11 QNYEKLRKICIKKKQPFVDTLFPP Q22036 13 13 QKYQELKQDCMKDGRLFCDPTFLP O88501 11 11 CALPAIN2 Length of motif = 23 Motif number = 2 Calpain motif II - 2 PCODE ST INT WKRPKEICENPRFIIGGANRTDI O08702 79 20 WKRPKEICENPRFIIGGANRTDI O70376 79 20 WKRPKEICENPRFIIGGANRTDI O70482 79 20 WKRPPEICENPRFIIGGANRTDI CAN3_MOUSE 99 16 WKRPPEICENPRFIIGGANRTDI CAN3_RAT 99 16 WKRPTEICADPQFIIGGATRTDI O54843 75 21 WKRPPEICENPRFIIGGANRTDI O46596 98 16 WKRPTEICADPQFIIGGATRTDI CAN2_RAT 75 21 WKKPKEICENPGFIIGGANRTDI O88977 79 20 WKRPTELMSNPQFIVDGATRTDI O35350 85 21 WKRPPEICENPRFIIDGANRTDI CAN3_HUMAN 99 16 WKRPTEICADPQFIIGGATRTDI CAN2_HUMAN 75 21 WKRPTEICADPQFIIGGATRTDI CAN2_MOUSE 75 21 WKRPTELMSNPQFIVDGATRTDI O88666 85 21 WKRPTELLSNPQFIVDGATRTDI P97571 85 21 WKRPTELLSNPQFIVDGATRTDI CAN1_HUMAN 85 21 WKRPREICENPRFIIGGANRTDI CAN3_CHICK 93 16 WCRPTELCSCPRFIAGGATRTDI CAN2_CHICK 75 21 WKRPTELCRHPQFIVDGATRTDI O42133 85 21 WKRPTELCPNPQFIVGGATRTDI Q64698 75 21 WKRPSELVDDPQFIVGGATRTDI CANX_CHICK 77 21 WKRPGEIVKNPEFILGGATRTDI O14815 67 16 WLRPHEIAENPQFFVEGYSRFDV CAN_DROME 113 16 WLRPGEITREPQLITEGHSRFDV YKR2_CAEEL 245 16 WKRPKDICDDPRLFVDGISSHDL O08688 51 16 WKRPHEINPNAKFFAGGASRFDI CAN_SCHMA 124 16 WKRPKGICEDPRLFVDGISSHDL O15484 51 16 WKRPQDISDDPHLIVGNISNHQL O35646 51 16 WKRPGELHPDPHLFVEGASPNDV Q22036 53 16 WKRPQDISDDPHLIVGNISNHQL O88501 51 16 CALPAIN3 Length of motif = 17 Motif number = 3 Calpain motif III - 2 PCODE ST INT QGDLGDCWFLAAIACLT O08702 103 1 QGDLGDCWFLAAIACLT O70376 103 1 QGDLGDCWFLAAIACLT O70482 103 1 QGDLGDCWFLAAIACLT CAN3_MOUSE 123 1 QGDLGDCWLLAAIACLT CAN3_RAT 123 1 QGALGDCWLLAAIASLT O54843 99 1 QGDLGDCWFLAAIACLT O46596 122 1 QGALGDCWLLAAIASLT CAN2_RAT 99 1 QGDLGDCWFLAAIACLT O88977 103 1 QGALGDCWLLAAIASLT O35350 109 1 QGELGDCWFLAAIACLT CAN3_HUMAN 123 1 QGALGDCWLLAAIASLT CAN2_HUMAN 99 1 QGALGDCWLLAAIASLT CAN2_MOUSE 99 1 QGALGDCWLLAAIASLT O88666 109 1 QGALGDCWLLAAIASLT P97571 109 1 QGALGDCWLLAAIASLT CAN1_HUMAN 109 1 QGELGDCWFLAAIACLT CAN3_CHICK 117 1 QGALGDCWLLAAIASLT CAN2_CHICK 99 1 QGALGDCWLLAAIASLT O42133 109 1 QGGLGDCWLLAAIASLT Q64698 99 1 QGALGDCWLLAAIGSLT CANX_CHICK 101 1 QGELGDCWLLAAIASLT O14815 91 1 QGELGDCWLLAATANLT CAN_DROME 137 1 QGELGDCWLLAAAANLT YKR2_CAEEL 269 1 QGQVGNCWFVAACSSLA O08688 75 1 QGALGDCWLLAVVASIS CAN_SCHMA 148 1 QGQVGNCWFVAACSSLA O15484 75 1 QGRLGNKAMISAFSCLA O35646 75 1 QGILGNCWFVSACSALT Q22036 77 1 QGRLGNKAMISAFSCLA O88501 75 1 CALPAIN4 Length of motif = 26 Motif number = 4 Calpain motif IV - 2 PCODE ST INT YAGIFHFQFWRYGDWVDVVIDDCLPT O08702 139 19 YAGIFHFQFWRYGDWVDVVIDDCLPT O70376 139 19 YAGIFHFQFWRYGDWVDVVIDDCLPT O70482 139 19 YAGIFHFQFWRYGDWVDVVIDDCLPT CAN3_MOUSE 159 19 YAGIFHFQFWRYGDWVDVVIDDCLPT CAN3_RAT 159 19 YAGIFHFQFWQYGEWVEVVVDDRLPT O54843 135 19 YAGIFHFQFWRYGDWVDVVIDDCLPT O46596 158 19 YAGIFHFQFWQYGEWVEVVVDDRLPT CAN2_RAT 135 19 YAGIFHFQFWRYGDWVDVVIDDCLPT O88977 139 19 YAGIFHFQLWQFGEWVDVVIDDLLPT O35350 145 19 YAGIFHFQFWRYGEWVDVVIDDCLPT CAN3_HUMAN 159 19 YAGIFHFQFWQYGEWVEVVVDDRLPT CAN2_HUMAN 135 19 YAGIFHFQFWQYGEWVEVVVDDRLPT CAN2_MOUSE 135 19 YAGIFHFQLWQFGEWVDVVIDDLLPT O88666 145 19 YAGIFHFQLWQFGEWVDVVVDDLLPT P97571 145 19 YAGIFHFQLWQFGEWVDVVVDDLLPI CAN1_HUMAN 145 19 YAGIFHFQFWRYGDWVDVIIDDCLPT CAN3_CHICK 153 19 YAGIFHFQFWQYGEWVDVVVDDRLPT CAN2_CHICK 135 19 YAGIFHFQIWQFGEWQDVVVDDYLPT O42133 145 19 YAGIFHFQFWQYGEWVEVVIDDRLPT Q64698 135 19 YAGIFHFQIWQFGEWVDVVVDDLLPT CANX_CHICK 137 19 YAGIFHFQFWQHSEWLDVVIDDRLPT O14815 127 19 YAGIFHFRFWQYGKWVDVIIDDRLPT CAN_DROME 173 19 YAGIFHFQFWQYGKWVDVVIDDRLPT YKR2_CAEEL 305 19 YAGIFHFNFWRFGEWVDVIVDDRLPT O08688 117 25 YVGVVRFRFWRFGHWVEVLIDDRLPV CAN_SCHMA 185 20 YAGIFHFHFWRLGMVDVVIDERLPTV O15484 117 25 YAGIFHFRFWHFGEWTEVVIDDLLPT O35646 117 25 YAGIFRFRFWRFGKWVEVVIDDLLPT Q22036 117 23 YAGIFRFRFWHFGEWTEVVIDDLLPT O88501 117 25 CALPAIN5 Length of motif = 24 Motif number = 5 Calpain motif V - 2 PCODE ST INT LVFTKSNHRNEFWSALLEKAYAKL O08702 169 4 LVFTKSNHRNEFWSALLEKAYAKL O70376 169 4 LVFTKSNHRNEFWSALLEKAYAKL O70482 169 4 LVFTKSNHRNEFWSALLEKAYAKL CAN3_MOUSE 189 4 LVFTKSNHRNEFWSALLEKAYAKL CAN3_RAT 189 4 LLFVHSAEGSEFWSALLEKAYAKI O54843 165 4 LVFTKSNHRNEFWSALLEKAYAKL O46596 188 4 LLFVHSAEGSEFWSALLEKAYAKI CAN2_RAT 165 4 LVFTKSNHRNEFWSALLEKAYAKL O88977 169 4 LVFVHSAQGNEFWSALLEKAYAKV O35350 175 4 LVFTKSNHRNEFWSALLEKAYAKL CAN3_HUMAN 189 4 LLFVHSAEGSEFWSALLEKAYAKI CAN2_HUMAN 165 4 LLFVHSAEGSEFWSALLEKAYAKI CAN2_MOUSE 165 4 LVFVHSAQGNEFWSALLEKAYAKV O88666 175 4 LVFVHSAQGNEFWSALLEKAYAKV P97571 175 4 LVFVHSAEGNEFWSALLEKAYAKV CAN1_HUMAN 175 4 LVFTKSSQRNEFWSALLEKAYAKL CAN3_CHICK 183 4 LLFVHSAEGSEFWSALLEKAYAKL CAN2_CHICK 165 4 LLFVHSAEGTEFWSALLEKAYAKV O42133 175 4 LLFLHSEEGNEFWSALLEKAYAKL Q64698 165 4 LLFVHSAECTEFWSALLEKAYAKL CANX_CHICK 167 4 LVFLHSADHNEFWSALLEKAYAKL O14815 157 4 LMYMHSTEKNEFWSALLEKAYAKL CAN_DROME 203 4 LLYMHSASNNEFWSALLEKAYAKL YKR2_CAEEL 335 4 LIYCHSNSKNEFWCALVEKAYAKL O08688 147 4 LVFMHSNDPTEFWSALLEKAYAKL CAN_SCHMA 217 6 LIYCHSNSRNEFWCALVEKAYAKL O15484 146 3 LVFSFSTSMNEFWNALLEKAYAKL O35646 147 4 LLFARSKTPNEFWSALLEKAFAKL Q22036 147 4 LVFSFSTSMNEFWNALLEKAYAKL O88501 147 4 CALPAIN6 Length of motif = 28 Motif number = 6 Calpain motif VI - 2 PCODE ST INT GSYEALKGGNTTEAMEDFTGGVTEFFEI O08702 194 1 GSYEALKGGNTTEAMEDFTGGVTEFFEI O70376 194 1 GSYEALKGGNTTEAMEDFTGGVTEFFEI O70482 194 1 GSYEALKGGNTTEAMEDFTGGVTEFFEI CAN3_MOUSE 214 1 GSYEALKGGNTTEAMEDFTGGVTEFFEI CAN3_RAT 214 1 GCYEALSGGATTEGFEDFTGGIAEWYEL O54843 190 1 GSYEALKGGNTTEAMEDFTGGVTEFFEI O46596 213 1 GCYEALSGGATTEGFEDFTGGIAEWYEL CAN2_RAT 190 1 GSYEALKGGNTTEAMEDFTGGVTEFFEI O88977 194 1 GSYEALSGGCTSEAFEDFTGGVTEWYDL O35350 200 1 GSYEALKGGNTTEAMEDFTGGVAEFFEI CAN3_HUMAN 214 1 GCYEALSGGATTEGFEDFTGGIAEWYEL CAN2_HUMAN 190 1 GCYETLSGGATTEGFEDFTGGIAEWYEL CAN2_MOUSE 190 1 GSYEALSGGCTSEAFEDFTGGVTEWYDL O88666 200 1 GSYEALSGGCTSEAFEDFTGGVTEWYDL P97571 200 1 GSYEALSGGSTSEGFEDFTGGVTEWYEL CAN1_HUMAN 200 1 GSYEALKGGNTTEAMEDFTGGVIEFYEI CAN3_CHICK 208 1 GSYEALSGGTTTEGFEDFTGGIAEWYEL CAN2_CHICK 190 1 GCYEALSGGSTSEGFEDFTGGVTEWYDL O42133 200 1 GSYEALVGGSTIEGFEDFTGGISEFYDL Q64698 190 1 GCYESLSGGSTTEGFEDFTGGVAEMYDL CANX_CHICK 192 1 GSYEALKGGSAIEAMEDFTGGVAETFQT O14815 182 1 GSYEALKGGSTCEAMEDFTGGVSEWYDL CAN_DROME 228 1 GSYEALKGGTTSEALEDMTGGLTEFIDL YKR2_CAEEL 360 1 GCYQALDGGNTADALVDFTGGVSEPIDL O08688 172 1 GCYAHLSGGSQSEAMEDLTGGICLSLEL CAN_SCHMA 242 1 GCYQALDGGNTADALVDFTGGVSEPIDL O15484 171 1 GCYEALDGLTITDIIMDFTGTLAEIIDM O35646 172 1 GCYENLVGGHLSDALQDVSGGVAETLHV Q22036 172 1 GCYEALDGLTITDIIMDFTGTLAEIIDM O88501 172 1 CALPAIN7 Length of motif = 22 Motif number = 7 Calpain motif VII - 2 PCODE ST INT EDGEFWMSYDDFVYHFTKLEIC O08702 325 103 EDGEFWMSYDDFVYHFTKLEIC O70376 325 103 EDGEFWMSYDDFVYHFTKLEIC O70482 373 151 EDGEFWMSYDDFVYHFTKLEIC CAN3_MOUSE 393 151 EDGEFWMSYDDFVYHFTKLEIC CAN3_RAT 393 151 EDGEFWMSFSDFLRHYSRLEIC O54843 320 102 EDGEFWMSYDDFIYHFTKLEIC O46596 393 152 EDGEFWMSFSDFLRHYSRLEIC CAN2_RAT 320 102 EDGEFWMSYDDFVYHFTKLEIC O88977 325 103 EDGEFWMSFRDFIREFTKLEIC O35350 330 102 EDGEFWMSYEDFIYHFTKLEIC CAN3_HUMAN 393 151 EDGEFWMSFSDFLRHYSRLEIC CAN2_HUMAN 320 102 EDGEFWMSFSDFLRHYSRLEIC CAN2_MOUSE 320 102 EDGEFWMSFRDFIREFTKLEIC O88666 330 102 EDGEFWMSFRDFIREFTKLEIC P97571 330 102 EDGEFWMSFRDFMREFTRLEIC CAN1_HUMAN 330 102 EDGEFWISLEDFMRHFTKLEIC CAN3_CHICK 386 150 EDGEFWMAFNDFLRHYSRLEIC CAN2_CHICK 320 102 EDGEFWMSFRDFLREFTRLEIC O42133 330 102 EDGEFWMSFSDFLKQYSRLEIC Q64698 320 102 EDGEFWMSFRDFMREFSRLEIC CANX_CHICK 322 102 DDGEFWMAFKDFKAHFDKVEIC O14815 313 103 RDGEFWMSFQDFLNHFDRVEIC CAN_DROME 363 107 HDGEFWMSFDDFMRNFEKMEIC YKR2_CAEEL 491 103 DDGEFWMTFEDMCRYFTDIIKC O08688 319 119 ADGEFWMSYEDFVTCFSRVEVC CAN_SCHMA 373 103 DDGEFWMTFEDVCRYFTDIIKC O15484 318 119 DDGEFWMSLEDFCHNFHKLNVC O35646 319 119 DDGDFWMPWESFVHYFTDISLC Q22036 329 129 DDGEFWMSLEDFCHNFHKLNVC O88501 319 119 CALPAIN8 Length of motif = 18 Motif number = 8 Calpain motif VIII - 2 PCODE ST INT SAGGCRNFPDTFWTNPQY O08702 375 28 SAGGCRNFPDTFWTNPQY O70376 375 28 SAGGCRNFPDTFWTNPQY O70482 423 28 SAGGCRNFPDTFWTNPQY CAN3_MOUSE 443 28 SAGGCRNFPDTFWTNPQY CAN3_RAT 443 28 TAGGCRNYPNTFWMNPQY O54843 370 28 SAGGCRNFPDTFWTNPQY O46596 443 28 TAGGCRNYPNTFWMNPQY CAN2_RAT 370 28 SAGGCRNFPDTFWTNPQY O88977 375 28 TAGGCRNYPATFWVNPQF O35350 380 28 SAGGCRNFPDTFWTNPQY CAN3_HUMAN 443 28 TAGGCRNYPNTFWMNPQY CAN2_HUMAN 370 28 TAGGCRNYPNTFWMNPQY CAN2_MOUSE 370 28 TAGGCRNYPATFWVNPQF O88666 380 28 TAGGCRNYPATFWVNPQF P97571 380 28 TAGGCRNYPATFWVNPQF CAN1_HUMAN 380 28 SAGGCRNYPDTFWTNPQY CAN3_CHICK 436 28 TAGGCRNYPNTFWTNPQY CAN2_CHICK 370 28 TAGGCRNYPATFWINPQF O42133 380 28 TAGGCLNYPGTYWTNPQF Q64698 370 28 TAGGCRNNPATFWINPQF CANX_CHICK 372 28 TAGGCRNFLDTFWTNPQI O14815 363 28 TAGGCRNFLDTFWHNPQY CAN_DROME 417 32 TAGGCRNYINTFANNPQF YKR2_CAEEL 549 36 RSGGCINHKDTFFQNPQY O08688 373 32 NAGGCINNRTTYWTSPQF CAN_SCHMA 427 32 RGGGCINHKDTFFQNPQY O15484 372 32 RSGGCYNNRDTFLQNPQY O35646 370 29 RAGGCHNFKATFCNNPQY Q22036 386 35 RSGGCYNNRDTFLQNPQY O88501 370 29 CALPAIN9 Length of motif = 29 Motif number = 9 Calpain motif IX - 2 PCODE ST INT RLPPSEYVIVPSTYEPHQEGEFILRVFSE O08702 480 87 RLPPSEYVIVPSTYEPHQEGEFILRVFSE O70376 480 87 RLPPSEYVIVPSTYEPHQEGEFILRVFSE O70482 528 87 RLPPSEYVIVPSTYEPHQEGEFILRVFSE CAN3_MOUSE 548 87 RLPPSEYVIVPSTYEPHQEGEFILRVFSE CAN3_RAT 548 87 KLPPGEYVLVPSTFEPHKDGDFCIRVFSE O54843 476 88 RLPPSEYVIVPSTYEPHQEGEFILRVFSE O46596 548 87 KLPPGEYVLVPSTFEPHKNGDFCIRVFSE CAN2_RAT 476 88 RLPPSEYVIVPSTYEPHQEGEFILRVFSE O88977 480 87 RLPPGEYIVVPSTFEPNKEGDFLLRFFSE O35350 487 89 RLPPSEYVIVPSTYEPHQEGEFILRVFSE CAN3_HUMAN 548 87 KLPPGEYILVPSTFEPNKDGDFCIRVFSE CAN2_HUMAN 476 88 KLPPGEYVLVPSTFEPHKDGDFCIRVFSE CAN2_MOUSE 476 88 RPPPGEYIVVPSTFEPNKEGDFLLRFFSE O88666 487 89 RLPPGEYIVVPSTFEPNKEGDFLLRFFSE P97571 487 89 RLPPGEYVVVPSTFEPNKEGDFVLRFFSE CAN1_HUMAN 488 90 RLPPSEYVIIPSTYEPHQEGEFILRVFSE CAN3_CHICK 541 87 KLPAGEYIIVPSTFEPNLNGDFCLRVFSE CAN2_CHICK 476 88 RLPPGEYIVVPSTFEPNREGDFVLRVFSE O42133 489 91 RLPPGQYLVVPSTFEPFKDGDFCLRVFSE Q64698 478 90 RLPPGEYIVVPSTFEPHKEADFILRVFTE CANX_CHICK 478 88 KLPPGEYILIPSTFEPHQEADFCLRIFSE O14815 460 79 KLPPGHYLIVPSTFDPNEEGEFIIRVFSE CAN_DROME 519 84 RVPPGNYVVVPSTFEPNEEAEFMLRVYTN YKR2_CAEEL 619 52 ELPEGRYVIIPTTFEPGHTGEFLLRVFTD O08688 462 71 RVPPGSYVVIPSTFDPNIEVNFILRVFSQ CAN_SCHMA 524 79 DQPEGRYVIIPTTFEPGHTGEFLLRVFTD O15484 461 71 YLKKGSYVLVPTMFQHGRTSEFLLRIFSE O35646 461 73 SLPRGRYLLIPTTFAPKEQTLFMLRVYSD Q22036 475 71 YLKKGNYVLVPTMFQHGRTSEFLLRIFSE O88501 461 73

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