WORKLIST ENTRIES (1):
CANABINOID1R View alignment View Structure Cannabinoid receptor type 1 signature
Type of fingerprint: COMPOUND with 7 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00362 CANNABINOIDR; PR00523 CANABINOID2R
INTERPRO; IPR000810
Creation date 19-MAY-1996; UPDATE 06-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Cannabinoid.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.80-83.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
The ability of marijuana to activate the cannabinoid receptor provides a
molecular explanation for its psychoactive effects and other CNS actions
(which include hallucinations, memory deficits, altered time and space
perception, CNS depression and appetite stimulation) [6]. The endogenous
ligand at the cannabinoid receptor is unknown, although it may be a
derivative of arachidonic acid. The cannabinoid receptor is widespread
throughout the CNS, high levels occurring in the dendate gyrus, hippocampus
and cerebral cortex, with more moderate levels in the hypothalamus and
amygdala. It is also present in various cell lines, and in the periphery
it is found in the testis and vas deferens [6].
CANABINOID1R is a 7-element fingerprint that provides a signature for type
1 cannabinoid receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the type 1 cannabinoid receptors but
distinguish them from the rest of the cannabinoid receptor family - motifs
1-3 lie in the N-terminal region immediately preceding TM domain 1; motif
4 lies in the third cytoplasmic loop; motif 5 spans the third external
loop; and motifs 6 and 7 lie in the C-terminal region following TM domain
7. A single iteration on OWL27.1 was required to reach convergence, no
further sequences being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 4 sequences, and 3
partial matches.
SUMMARY INFORMATION
4 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
2 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
7| 4 4 4 4 4 4 4
6| 0 0 0 0 0 0 0
5| 0 0 1 1 1 1 1
4| 2 0 0 1 2 2 1
3| 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0
--+------------------------------------
| 1 2 3 4 5 6 7
True positives..
CB1R_RAT CB1R_MOUSE CB1R_HUMAN CB1R_FELCA
Subfamily: Codes involving 5 elements
Subfamily True positives..
Q13949
Subfamily: Codes involving 4 elements
Subfamily True positives..
CB1A_FUGRU CB1B_FUGRU
PROTEIN TITLES
CB1R_RAT CANNABINOID RECEPTOR 1 (CB1) (CB-R) (BRAIN-TYPE CANNABINOID
CB1R_MOUSE CANNABINOID RECEPTOR 1 (CB1) (CB-R) (BRAIN-TYPE CANNABINOID
CB1R_HUMAN CANNABINOID RECEPTOR 1 (CB1) (CB-R) (CANN6) - HOMO SAPIENS (
CB1R_FELCA CANNABINOID RECEPTOR 1 (CB1) (CB-R) - FELIS SILVESTRIS CATUS
Q13949 CENTRAL CANNABINOID RECEPTOR - HOMO SAPIENS (HUMAN).
CB1A_FUGRU CANNABINOID RECEPTOR TYPE 1A - FUGU RUBRIPES (JAPANESE PUFFE
CB1B_FUGRU CANNABINOID RECEPTOR TYPE 1B - FUGU RUBRIPES (JAPANESE PUFFE
SCAN HISTORY
OWL27_1 1 100 NSINGLE
SPTR37_9f 2 23 NSINGLE
INITIAL MOTIF SETS
CANABINOID1R1 Length of motif = 20 Motif number = 1
Cannabinoid receptor 1 motif I - 1
PCODE ST INT
FRTITTDLLYVGSNDIQYED CB1R_HUMAN 13 13
FRTITTDLLYVGSNDIQYED CB1R_RAT 13 13
FRTITTDLLYVGSNDIQYED MMU17985 13 13
CANABINOID1R2 Length of motif = 23 Motif number = 2
Cannabinoid receptor 1 motif II - 1
PCODE ST INT
DMASKLGYFPQKFPLTSFRGSPF CB1R_HUMAN 36 3
DMASKLGYFPQKFPLTSFRGSPF CB1R_RAT 36 3
DMASKLGYFPQKFPLTSFRGSPF MMU17985 36 3
CANABINOID1R3 Length of motif = 20 Motif number = 3
Cannabinoid receptor 1 motif III - 1
PCODE ST INT
TEFYNKSLSSFKENEENIQC CB1R_HUMAN 79 20
TEFYNKSLSSFKENEENIQC CB1R_RAT 80 21
TEFYNKSLSSFKENEDNIQC MMU17985 80 21
CANABINOID1R4 Length of motif = 20 Motif number = 4
Cannabinoid receptor 1 motif IV - 1
PCODE ST INT
RMIQRGTQKSIIIHTSEDGK CB1R_HUMAN 307 208
RMIQRGTQKSIIIHTSEDGK CB1R_RAT 308 208
RMIQRGTQKSIIIHTSEDGK MMU17985 308 208
CANABINOID1R5 Length of motif = 12 Motif number = 5
Cannabinoid receptor 1 motif V - 1
PCODE ST INT
VYDVFGKMNKLI CB1R_HUMAN 364 37
VYDVFGKMNKLI CB1R_RAT 365 37
VYDVFGKMNKLI MMU17985 365 37
CANABINOID1R6 Length of motif = 18 Motif number = 6
Cannabinoid receptor 1 motif VI - 1
PCODE ST INT
HAFRSMFPSCEGTAQPLD CB1R_HUMAN 406 30
HAFRSMFPSCEGTAQPLD CB1R_RAT 407 30
HAFRSMFPSCEGTAQPLD MMU17985 407 30
CANABINOID1R7 Length of motif = 20 Motif number = 7
Cannabinoid receptor 1 motif VII - 1
PCODE ST INT
HKHANNAASVHRAAESCIKS CB1R_HUMAN 433 9
HKHANNTASMHRAAESCIKS CB1R_RAT 434 9
HKHANNTASMHRAAESCIKS MMU17985 434 9
FINAL MOTIF SETS
CANABINOID1R1 Length of motif = 20 Motif number = 1
Cannabinoid receptor 1 motif I - 2
PCODE ST INT
FRTITTDLLYVGSNDIQYED CB1R_RAT 13 13
FRTITTDLLYVGSNDIQYED CB1R_MOUSE 13 13
FRTITTDLLYVGSNDIQYED CB1R_HUMAN 13 13
FRTITTDLLYVGSNDIQYED CB1R_FELCA 13 13
CANABINOID1R2 Length of motif = 23 Motif number = 2
Cannabinoid receptor 1 motif II - 2
PCODE ST INT
DMASKLGYFPQKFPLTSFRGSPF CB1R_RAT 36 3
DMASKLGYFPQKFPLTSFRGSPF CB1R_MOUSE 36 3
DMASKLGYFPQKFPLTSFRGSPF CB1R_HUMAN 36 3
DMASKLGYFPQKFPLTSFRGSPF CB1R_FELCA 36 3
CANABINOID1R3 Length of motif = 20 Motif number = 3
Cannabinoid receptor 1 motif III - 2
PCODE ST INT
TEFYNKSLSSFKENEENIQC CB1R_RAT 80 21
TEFYNKSLSSFKENEDNIQC CB1R_MOUSE 80 21
TEFYNKSLSSFKENEENIQC CB1R_HUMAN 79 20
TEFYNKSLSSYKENEENIQC CB1R_FELCA 79 20
CANABINOID1R4 Length of motif = 20 Motif number = 4
Cannabinoid receptor 1 motif IV - 2
PCODE ST INT
RMIQRGTQKSIIIHTSEDGK CB1R_RAT 308 208
RMIQRGTQKSIIIHTSEDGK CB1R_MOUSE 308 208
RMIQRGTQKSIIIHTSEDGK CB1R_HUMAN 307 208
RMIQRGTQKSIIIHTSEDGK CB1R_FELCA 307 208
CANABINOID1R5 Length of motif = 12 Motif number = 5
Cannabinoid receptor 1 motif V - 2
PCODE ST INT
VYDVFGKMNKLI CB1R_RAT 365 37
VYDVFGKMNKLI CB1R_MOUSE 365 37
VYDVFGKMNKLI CB1R_HUMAN 364 37
VYDVFGKMNKLI CB1R_FELCA 364 37
CANABINOID1R6 Length of motif = 18 Motif number = 6
Cannabinoid receptor 1 motif VI - 2
PCODE ST INT
HAFRSMFPSCEGTAQPLD CB1R_RAT 407 30
HAFRSMFPSCEGTAQPLD CB1R_MOUSE 407 30
HAFRSMFPSCEGTAQPLD CB1R_HUMAN 406 30
HAFRSMFPSCEGTAQPLD CB1R_FELCA 406 30
CANABINOID1R7 Length of motif = 20 Motif number = 7
Cannabinoid receptor 1 motif VII - 2
PCODE ST INT
HKHANNTASMHRAAESCIKS CB1R_RAT 434 9
HKHANNTASMHRAAESCIKS CB1R_MOUSE 434 9
HKHANNAASVHRAAESCIKS CB1R_HUMAN 433 9
HKHANNTANVHRAAENCIKN CB1R_FELCA 433 9
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