WORKLIST ENTRIES (1):
CANNABINOIDR View alignment View Structure Cannabinoid receptor signature
Type of fingerprint: COMPOUND with 9 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00522 CANABINOID1R; PR00523 CANABINOID2R
INTERPRO; IPR002230
Creation date 05-JUL-1995; UPDATE 06-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Cannabinoid.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.80-83.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
The ability of marijuana to activate the cannabinoid receptor provides a
molecular explanation for its psychoactive effects and other CNS actions
(which include hallucinations, memory deficits, altered time and space
perception, CNS depression and appetite stimulation) [6]. The endogenous
ligand at the cannabinoid receptor is unknown, although it may be a
derivative of arachidonic acid. The cannabinoid receptor is widespread
throughout the CNS, high levels occurring in the dendate gyrus, hippocampus
and cerebral cortex, with more moderate levels in the hypothalamus and
amygdala. It is also present in various cell lines, and in the periphery
it is found in the testis and vas deferens [6].
CANNABINOIDR is a 9-element fingerprint that provides a signature for the
cannabinoid receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the
alignment that characterise the cannabinoid receptors but distinguish them
from the rest of the rhodopsin-like superfamily - motif 1 lies in the
N-terminal region immediately preceding TM domain 1; motif 2 spans the
central portion of TM domain 1; motif 3 lies in the first cytoplasmic loop;
motif 4 spans the C-terminus of TM domain 2 and the first external loop;
motif 5 lies in the second external loop; motif 6 lies at the C-terminus of
TM domain 5; motif 7 was drawn from the third cytoplasmic loop; and motifs
8 and 9 lie at the N- and C-termini of TM domain 7. Two iterations on
OWL27.1 were required to reach convergence, at which point a true set
comprising 6 sequences was identfied. Several partial matches were also
found, all of which are members of the melanocortin/melanotropin receptor
family that match motifs 2 and 9.
An update on SPTR37_9f identified a true set of 9 sequences.
SUMMARY INFORMATION
9 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
9| 9 9 9 9 9 9 9 9 9
8| 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0 0
--+----------------------------------------------
| 1 2 3 4 5 6 7 8 9
True positives..
CB1R_MOUSE CB1R_RAT CB1R_FELCA CB1R_HUMAN
Q13949 CB1A_FUGRU CB1B_FUGRU CB2R_MOUSE
CB2R_HUMAN
PROTEIN TITLES
CB1R_MOUSE CANNABINOID RECEPTOR 1 (CB1) (CB-R) (BRAIN-TYPE CANNABINOID
CB1R_RAT CANNABINOID RECEPTOR 1 (CB1) (CB-R) (BRAIN-TYPE CANNABINOID
CB1R_FELCA CANNABINOID RECEPTOR 1 (CB1) (CB-R) - FELIS SILVESTRIS CATUS
CB1R_HUMAN CANNABINOID RECEPTOR 1 (CB1) (CB-R) (CANN6) - HOMO SAPIENS (
Q13949 CENTRAL CANNABINOID RECEPTOR - HOMO SAPIENS (HUMAN).
CB1A_FUGRU CANNABINOID RECEPTOR TYPE 1A - FUGU RUBRIPES (JAPANESE PUFFE
CB1B_FUGRU CANNABINOID RECEPTOR TYPE 1B - FUGU RUBRIPES (JAPANESE PUFFE
CB2R_MOUSE CANNABINOID RECEPTOR 2 (CB2) (CB-2) - MUS MUSCULUS (MOUSE).
CB2R_HUMAN CANNABINOID RECEPTOR 2 (CB2) (CB-2) (CX5) - HOMO SAPIENS (HU
SCAN HISTORY
OWL27_1 2 75 NSINGLE
SPTR37_9f 2 10 NSINGLE
INITIAL MOTIF SETS
CANNABINOIDR1 Length of motif = 14 Motif number = 1
Cannabinoid receptor motif I - 1
PCODE ST INT
MECFMILNPSQQLA CB1R_RAT 106 106
IECFMVLNPSQQLA CB1R_HUMAN 105 105
MKDYMILSGPQKTA CB2R_HUMAN 22 22
CANNABINOIDR2 Length of motif = 14 Motif number = 2
Cannabinoid receptor motif II - 1
PCODE ST INT
LGTFTVLENLLVLC CB1R_RAT 127 7
LGTFTVLENLLVLC CB1R_HUMAN 126 7
LGLLSALENVAVLY CB2R_HUMAN 43 7
CANNABINOIDR3 Length of motif = 14 Motif number = 3
Cannabinoid receptor motif III - 1
PCODE ST INT
HSRSLRCRPSYHFI CB1R_RAT 144 3
HSRSLRCRPSYHFI CB1R_HUMAN 143 3
SSHQLRRKPSYLFI CB2R_HUMAN 60 3
CANNABINOIDR4 Length of motif = 23 Motif number = 4
Cannabinoid receptor motif IV - 1
PCODE ST INT
FVYSFVDFHVFHRKDSPNVFLFK CB1R_RAT 171 13
FVYSFIDFHVFHRKDSRNVFLFK CB1R_HUMAN 170 13
FACSFVNFHVFHGVDSKAVFLLK CB2R_HUMAN 87 13
CANNABINOIDR5 Length of motif = 16 Motif number = 5
Cannabinoid receptor motif V - 1
PCODE ST INT
CSDIFPLIDETYLMFW CB1R_RAT 265 71
CSDIFPHIDETYLMFW CB1R_HUMAN 264 71
CSELFPLIPNDYLLSW CB2R_HUMAN 179 69
CANNABINOIDR6 Length of motif = 16 Motif number = 6
Cannabinoid receptor motif VI - 1
PCODE ST INT
YAYMYILWKAHSHAVR CB1R_RAT 293 12
YAYMYILWKAHSHAVR CB1R_HUMAN 292 12
YTYGHVLWKAHQHVAS CB2R_HUMAN 207 12
CANNABINOIDR7 Length of motif = 15 Motif number = 7
Cannabinoid receptor motif VII - 1
PCODE ST INT
VQVTRPDQARMDIRL CB1R_RAT 328 19
VQVTRPDQARMDIRL CB1R_HUMAN 327 19
RQVPGMARMRLDVRL CB2R_HUMAN 229 6
CANNABINOIDR8 Length of motif = 12 Motif number = 8
Cannabinoid receptor motif VIII - 1
PCODE ST INT
IKTVFAFCSMLC CB1R_RAT 376 33
IKTVFAFCSMLC CB1R_HUMAN 375 33
VKKAFAFCSMLC CB2R_HUMAN 277 33
CANNABINOIDR9 Length of motif = 11 Motif number = 9
Cannabinoid receptor motif IX - 1
PCODE ST INT
YALRSKDLRHA CB1R_RAT 398 10
YALRSKDLRHA CB1R_HUMAN 397 10
YALRSGEIRSS CB2R_HUMAN 299 10
FINAL MOTIF SETS
CANNABINOIDR1 Length of motif = 14 Motif number = 1
Cannabinoid receptor motif I - 2
PCODE ST INT
MECFMILNPSQQLA CB1R_MOUSE 106 106
MECFMILNPSQQLA CB1R_RAT 106 106
MECFMILNPSQQLA CB1R_FELCA 105 105
IECFMVLNPSQQLA CB1R_HUMAN 105 105
IECFMVLNPSQQLA Q13949 44 44
MECFMILTPSQQLA CB1A_FUGRU 104 104
MECFMILTPAQQLV CB1B_FUGRU 102 102
MKEYMILSSGQQIA CB2R_MOUSE 22 22
MKDYMILSGPQKTA CB2R_HUMAN 22 22
CANNABINOIDR2 Length of motif = 14 Motif number = 2
Cannabinoid receptor motif II - 2
PCODE ST INT
LGTFTVLENLLVLC CB1R_MOUSE 127 7
LGTFTVLENLLVLC CB1R_RAT 127 7
LGTFTVLENLLVLC CB1R_FELCA 126 7
LGTFTVLENLLVLC CB1R_HUMAN 126 7
LGTFTVLENLLVLC Q13949 65 7
LGTFTVLENLVVLC CB1A_FUGRU 125 7
LGTFTVLENFVVLC CB1B_FUGRU 123 7
MGLLSALENMAVLY CB2R_MOUSE 43 7
LGLLSALENVAVLY CB2R_HUMAN 43 7
CANNABINOIDR3 Length of motif = 14 Motif number = 3
Cannabinoid receptor motif III - 2
PCODE ST INT
HSRSLRCRPSYHFI CB1R_MOUSE 144 3
HSRSLRCRPSYHFI CB1R_RAT 144 3
HSRSLRCRPSYHFI CB1R_FELCA 143 3
HSRSLRCRPSYHFI CB1R_HUMAN 143 3
HSRSLRCRPSYHFI Q13949 82 3
QSRTLRCRPSYHFI CB1A_FUGRU 142 3
HSHTLRSRPSYHFI CB1B_FUGRU 140 3
SSRRLRRKPSYLFI CB2R_MOUSE 60 3
SSHQLRRKPSYLFI CB2R_HUMAN 60 3
CANNABINOIDR4 Length of motif = 23 Motif number = 4
Cannabinoid receptor motif IV - 2
PCODE ST INT
FVYSFVDFHVFHRKDSPNVFLFK CB1R_MOUSE 171 13
FVYSFVDFHVFHRKDSPNVFLFK CB1R_RAT 171 13
FVYSFVDFHVFHRKDSPNVFLFK CB1R_FELCA 170 13
FVYSFIDFHVFHRKDSRNVFLFK CB1R_HUMAN 170 13
FVYSFIDFHVFHRKDSRNVFLFK Q13949 109 13
FVYSFLDFHVFHKKDSPNVFLFK CB1A_FUGRU 169 13
FVYSFLDFHVLHRKDSPSIFLFK CB1B_FUGRU 167 13
FACNFVIFHVFHGVDSNAIFLLK CB2R_MOUSE 87 13
FACSFVNFHVFHGVDSKAVFLLK CB2R_HUMAN 87 13
CANNABINOIDR5 Length of motif = 16 Motif number = 5
Cannabinoid receptor motif V - 2
PCODE ST INT
CSDIFPLIDETYLMFW CB1R_MOUSE 265 71
CSDIFPLIDETYLMFW CB1R_RAT 265 71
CSDIFPLIDETYLMFW CB1R_FELCA 264 71
CSDIFPHIDETYLMFW CB1R_HUMAN 264 71
CSDIFPHIDETYLMFW Q13949 203 71
CSDIFPLIDENYLMFW CB1A_FUGRU 263 71
CSDIFPLIDEKYLMFW CB1B_FUGRU 261 71
CSELFPLIPNDYLLGW CB2R_MOUSE 179 69
CSELFPLIPNDYLLSW CB2R_HUMAN 179 69
CANNABINOIDR6 Length of motif = 16 Motif number = 6
Cannabinoid receptor motif VI - 2
PCODE ST INT
YAYMYILWKAHSHAVR CB1R_MOUSE 293 12
YAYMYILWKAHSHAVR CB1R_RAT 293 12
YAYMYILWKAHIHAVR CB1R_FELCA 292 12
YAYMYILWKAHSHAVR CB1R_HUMAN 292 12
YAYMYILWKAHSHAVR Q13949 231 12
YAYIYILWKAHHHAVR CB1A_FUGRU 291 12
YAYMFILWKSHHHAVR CB1B_FUGRU 289 12
YTYGYVLWKAHRHVAT CB2R_MOUSE 207 12
YTYGHVLWKAHQHVAS CB2R_HUMAN 207 12
CANNABINOIDR7 Length of motif = 15 Motif number = 7
Cannabinoid receptor motif VII - 2
PCODE ST INT
VQVTRPDQARMDIRL CB1R_MOUSE 328 19
VQVTRPDQARMDIRL CB1R_RAT 328 19
VQVTRPDQARMDIRL CB1R_FELCA 327 19
VQVTRPDQARMDIRL CB1R_HUMAN 327 19
VQVTRPDQARMDIRL Q13949 266 19
VQTTRPEQTRMDIRL CB1A_FUGRU 326 19
VQTVRPEQARMDLRL CB1B_FUGRU 324 19
RQVPGIARMRLDVRL CB2R_MOUSE 229 6
RQVPGMARMRLDVRL CB2R_HUMAN 229 6
CANNABINOIDR8 Length of motif = 12 Motif number = 8
Cannabinoid receptor motif VIII - 2
PCODE ST INT
IKTVFAFCSMLC CB1R_MOUSE 376 33
IKTVFAFCSMLC CB1R_RAT 376 33
IKTVFAFCSMLC CB1R_FELCA 375 33
IKTVFAFCSMLC CB1R_HUMAN 375 33
IKTVFAFCSMLC Q13949 314 33
IKTVFAFCSMLC CB1A_FUGRU 374 33
IKTVFAFCSMLC CB1B_FUGRU 372 33
VKEAFAFCSMLC CB2R_MOUSE 277 33
VKKAFAFCSMLC CB2R_HUMAN 277 33
CANNABINOIDR9 Length of motif = 11 Motif number = 9
Cannabinoid receptor motif IX - 2
PCODE ST INT
YALRSKDLRHA CB1R_MOUSE 398 10
YALRSKDLRHA CB1R_RAT 398 10
YALRSKDLRHA CB1R_FELCA 397 10
YALRSKDLRHA CB1R_HUMAN 397 10
YALRSKDLRHA Q13949 336 10
YALRSRDLRHA CB1A_FUGRU 396 10
YAMRSKDLRRA CB1B_FUGRU 394 10
YALRSGEIRSA CB2R_MOUSE 299 10
YALRSGEIRSS CB2R_HUMAN 299 10
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