WORKLIST ENTRIES (1):
CXCCHMKINER5 View alignment View Structure C-X-C chemokine receptor type 5 signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00899 GPCRSTE3; PR00250 GPCRSTE2
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR01532 CXCCHMKINER3; PR00645 CXCCHMKINER4; PR01105 CXCCHMKINER6
INTERPRO; IPR001053
Creation date 13-SEP-1996; UPDATE 04-JUN-2001
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Chemokines.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.83-88.
7. DOBNER, T., WOLF, I., EMRICH, T. AND LIPP, M.
Differentiation-specific expression of a novel G protein-coupled receptor
from Burkitt's lymphoma.
EUR.J.IMMUNOL. 22 2795-9 (1992).
8. LEGLER, D.F., LOETSCHER, M., ROOS, R.S., CLARK-LEWIS, I., BAGGIOLINI, M.
AND MOSER, B.
B cell-attracting chemokine 1, a human CXC chemokine expressed in lymphoid
tissues, selectively attracts B lymphocytes via BLR1/CXCR5.
J.EXP.MED. 187(4) 655-660 (1998).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Chemokines are proteins that have important physiological and patho-
physiological roles in a wide range of acute and chronic inflammatory
processes [6]. Their sequences are similar and are characterised by a
4-cysteine motif: the family can be divided according to whether the first 2
Cys residues are adjacent (the C-C family), or separated by an intervening
residue (the C-x-C family).
A novel C-X-C chemokine, named B cell-attracting chemokine 1 (BCA-1), was
identified through screening of expressed sequence tag data [8]. The
chemokine was found to be expressed at highest levels in the liver, spleen,
lymph nodes, appendix and stomach. Lower levels of BCA-1 were also found in
the salivary and mammary glands. Expression appears to be constitutive in
lymphoid tissues [8].
The receptor for BCA-1 was found to be a previously identified orphan
receptor, expressed on B lymphocytes and Burkitt's lymphoma cells. This
receptor, originally named BLR1 (Burkitt's lymphoma receptor 1), has now
been renamed CXCR5 (C-X-C chemokine receptor type 5)[8]. B lymphocytes
expressing CXCR5 migrate in a concentration dependent manner in response to
BCA-1. BCA-1 does not induce chemotaxis in T lymphocytes, monocytes or
neutrophils. This selectivity for B lymphocytes is unique among the
chemokines [8]. BCA-l/CXCR5 may be important for the development of B
cell areas of secondary lymphoid tissue; deletion of the CXCR5 gene in
mice results in animals lacking inguinal lymph nodes and having defective
formation of primary follicles and germinal centres of the spleen and Peyer's
patches. Receptor-deficient B cells enter T cell areas but do not migrate
into B cell areas [8]. The expression of CXCR5 in Burkitt's lymphoma cells
also suggests a role for the receptor in lymphomagenesis [7].
CXCCHMKINER5 is an 8-element fingerprint that provides a signature for
type 5 C-X-C chemokine receptor. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the type 5 C-X-C chemokine receptors but
distinguish them from the rest of the chemokine receptor family - motifs 1
and 2 lie at the N-terminus; motif 3 spans the first cytoplasmic loop; motif
4 spans the second cytoplasmic loop; motif 5 lies in the second external
loop; motif 6 spans the third cytoplasmic loop; and motifs 7 and 8 lie at
the C-terminus. A single iteration on SPTR39_15f was required to reach
convergence, no further sequences being identified beyond the starting set.
A single partial match was found, O93281, a closely related receptor from
chicken that matches motifs 3, 4 and 6.
SUMMARY INFORMATION
3 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 3 3 3 3 3 3 3 3
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 1 1 0 1 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
CCR5_MOUSE CCR5_RAT CCR5_HUMAN
Subfamily: Codes involving 3 elements
Subfamily True positives..
O93281
PROTEIN TITLES
CCR5_MOUSE C-X-C CHEMOKINE RECEPTOR TYPE 5 (CXC-R5) (CXCR-5) (BURKITT'S
CCR5_RAT C-X-C CHEMOKINE RECEPTOR TYPE 5 (CXC-R5) (CXCR-5) (BURKITT'S
CCR5_HUMAN C-X-C CHEMOKINE RECEPTOR TYPE 5 (CXC-R5) (CXCR-5) (BURKITT'S
O93281 PUTATIVE CHEMOKINE RECEPTOR - Gallus gallus (Chicken).
SCAN HISTORY
SPTR39_15f 1 150 NSINGLE
INITIAL MOTIF SETS
CXCCHMKINER51 Length of motif = 17 Motif number = 1
C-X-C chemokine receptor type 5 motif I - 1
PCODE ST INT
NMDDLYKELAFYSNSTE CCR5_MOUSE 15 15
NMDDLYKELAIYSNSTE CCR5_RAT 15 15
NLEDLFWELDRLDNYND CCR5_HUMAN 13 13
CXCCHMKINER52 Length of motif = 17 Motif number = 2
C-X-C chemokine receptor type 5 motif II - 1
PCODE ST INT
DSNFCSTVEGPLLTSFK CCR5_MOUSE 36 4
DSIFCSTEEGPLLTSFK CCR5_RAT 36 4
ENHLCPATEGPLMASFK CCR5_HUMAN 34 4
CXCCHMKINER53 Length of motif = 16 Motif number = 3
C-X-C chemokine receptor type 5 motif III - 1
PCODE ST INT
ERHRHTRSSTETFLFH CCR5_MOUSE 79 26
ERHRHTRSSTETFLFH CCR5_RAT 79 26
ERHRQTRSSTETFLFH CCR5_HUMAN 77 26
CXCCHMKINER54 Length of motif = 15 Motif number = 4
C-X-C chemokine receptor type 5 motif IV - 1
PCODE ST INT
HAYRRRRLLSIHITC CCR5_MOUSE 158 63
HAYRRRRLLSIHITC CCR5_RAT 158 63
HAYRHRRLLSIHITC CCR5_HUMAN 156 63
CXCCHMKINER55 Length of motif = 18 Motif number = 5
C-X-C chemokine receptor type 5 motif V - 1
PCODE ST INT
SLPQCTFSQENEAETRAW CCR5_MOUSE 200 27
SLPQCIFSQENEAETRAW CCR5_RAT 200 27
SLPRCTFSQENQAETHAW CCR5_HUMAN 198 27
CXCCHMKINER56 Length of motif = 17 Motif number = 6
C-X-C chemokine receptor type 5 motif VI - 1
PCODE ST INT
HRLLQAQRRPQRQKAVR CCR5_MOUSE 245 27
HRLLQAQRRPQRQKAVR CCR5_RAT 245 27
HRLRQAQRRPQRQKAVR CCR5_HUMAN 243 27
CXCCHMKINER57 Length of motif = 18 Motif number = 7
C-X-C chemokine receptor type 5 motif VII - 1
PCODE ST INT
RLLTKLGCAGPASLCQLF CCR5_MOUSE 337 75
RLLTKLGCAGPASLCQLF CCR5_RAT 337 75
RLLTKLGCTGPASLCQLF CCR5_HUMAN 335 75
CXCCHMKINER58 Length of motif = 19 Motif number = 8
C-X-C chemokine receptor type 5 motif VIII - 1
PCODE ST INT
FPNWRKSSLSESENATSLT CCR5_MOUSE 354 -1
FPGWRKSSLSESENATSLT CCR5_RAT 354 -1
FPSWRRSSLSESENATSLT CCR5_HUMAN 352 -1
FINAL MOTIF SETS
CXCCHMKINER51 Length of motif = 17 Motif number = 1
C-X-C chemokine receptor type 5 motif I - 1
PCODE ST INT
NMDDLYKELAFYSNSTE CCR5_MOUSE 15 15
NMDDLYKELAIYSNSTE CCR5_RAT 15 15
NLEDLFWELDRLDNYND CCR5_HUMAN 13 13
CXCCHMKINER52 Length of motif = 17 Motif number = 2
C-X-C chemokine receptor type 5 motif II - 1
PCODE ST INT
DSNFCSTVEGPLLTSFK CCR5_MOUSE 36 4
DSIFCSTEEGPLLTSFK CCR5_RAT 36 4
ENHLCPATEGPLMASFK CCR5_HUMAN 34 4
CXCCHMKINER53 Length of motif = 16 Motif number = 3
C-X-C chemokine receptor type 5 motif III - 1
PCODE ST INT
ERHRHTRSSTETFLFH CCR5_MOUSE 79 26
ERHRHTRSSTETFLFH CCR5_RAT 79 26
ERHRQTRSSTETFLFH CCR5_HUMAN 77 26
CXCCHMKINER54 Length of motif = 15 Motif number = 4
C-X-C chemokine receptor type 5 motif IV - 1
PCODE ST INT
HAYRRRRLLSIHITC CCR5_MOUSE 158 63
HAYRRRRLLSIHITC CCR5_RAT 158 63
HAYRHRRLLSIHITC CCR5_HUMAN 156 63
CXCCHMKINER55 Length of motif = 18 Motif number = 5
C-X-C chemokine receptor type 5 motif V - 1
PCODE ST INT
SLPQCTFSQENEAETRAW CCR5_MOUSE 200 27
SLPQCIFSQENEAETRAW CCR5_RAT 200 27
SLPRCTFSQENQAETHAW CCR5_HUMAN 198 27
CXCCHMKINER56 Length of motif = 17 Motif number = 6
C-X-C chemokine receptor type 5 motif VI - 1
PCODE ST INT
HRLLQAQRRPQRQKAVR CCR5_MOUSE 245 27
HRLLQAQRRPQRQKAVR CCR5_RAT 245 27
HRLRQAQRRPQRQKAVR CCR5_HUMAN 243 27
CXCCHMKINER57 Length of motif = 18 Motif number = 7
C-X-C chemokine receptor type 5 motif VII - 1
PCODE ST INT
RLLTKLGCAGPASLCQLF CCR5_MOUSE 337 75
RLLTKLGCAGPASLCQLF CCR5_RAT 337 75
RLLTKLGCTGPASLCQLF CCR5_HUMAN 335 75
CXCCHMKINER58 Length of motif = 19 Motif number = 8
C-X-C chemokine receptor type 5 motif VIII - 1
PCODE ST INT
FPNWRKSSLSESENATSLT CCR5_MOUSE 354 -1
FPGWRKSSLSESENATSLT CCR5_RAT 354 -1
FPSWRRSSLSESENATSLT CCR5_HUMAN 352 -1
User query: Display/Full Code "CXCCHMKINER5"