WORKLIST ENTRIES (1):
DOPAMINED2R View alignment View Structure Dopamine D2 receptor signature
Type of fingerprint: COMPOUND with 6 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00242 DOPAMINER; PR00565 DOPAMINED1AR; PR00566 DOPAMINED1BR
PRINTS; PR00568 DOPAMINED3R; PR00569 DOPAMINED4R
INTERPRO; IPR001922
Creation date 17-AUG-1996; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. GRANDY, D.K., MARCHIONNI, M.A., MAKAM, H., STOFKO, R.E., ALFANO, M.,
FROTHINGHAM, L., FISCHER, J.B., BURKE-HOWIE, K.J., BUNZOW, J.R.,
SERVER, A.C. AND CIVELLI, O.
Cloning of the cDNA and gene for a human D2 dopamine receptor.
PROC.NATL.ACAD.SCI.U.S.A. 86 9762-9766 (1989).
7. WATSON, S. AND ARKINSTALL, S.
Dopamine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.96-110.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Dopamine neurons in the vertebrate central nervous system are involved in
the initiation and execution of movement, the maintenance of emotional
stability, and the regulation of pituitary function [6]. Various human
neurological diseases (e.g., Parkinson disease and schizophrenia), are
believed to be manifestations of dopamine and dopamine receptor imbalance.
The receptors have been divided into several different subtypes (designated
D1-D5), which may be distinguished by their G protein coupling, ligand
specificity, anatomical distribution and physiological effects.
D2 receptors have a similar pharmacological profile to D3 and D4 receptors.
They are present in high levels in the principal dopamine projection areas
(including the caudate-putamen, nucleus accumbens and olfactory tubercle);
they are found in cell bodies of dopaminergic neurons in the substantia
nigra and ventral tegmental area; and, in the periphery, they are found in
the pituitary, heart and blood vessels [7].
DOPAMINED2R is a 6-element fingerprint that provides a signature for the
D2 family of dopamine receptors. The fingerprint was derived from an initial
alignment of 6 sequences: the motifs were drawn from conserved sections
within either loop or N-terminal regions, focusing on those areas of the
alignment that characterise the D2 receptors but distinguish them from the
rest of the rhodopsin-like superfamily - motif 1 lies at the N-terminus;
motifs 2-5 span the third cytoplasmic loop; and motif 6 spans the third
external loop. A single iteration on OWL28.1 was required to reach
convergence, no further sequences being identified beyond the starting set.
A single partial match was also found, D2D2_XENLA, a D2 receptor fragment
lacking the first motif.
An update on SPTR37_9f identified a true set of 6 sequences.
SUMMARY INFORMATION
6 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
6| 6 6 6 6 6 6
5| 0 0 0 0 0 0
4| 0 0 0 0 0 0
3| 0 0 0 0 0 0
2| 0 0 0 0 0 0
--+-------------------------------
| 1 2 3 4 5 6
True positives..
D2DR_CERAE D2DR_HUMAN D2DR_BOVIN D2DR_MOUSE
O73810 D2D1_XENLA
PROTEIN TITLES
D2DR_CERAE D(2) DOPAMINE RECEPTOR - CERCOPITHECUS AETHIOPS (GREEN MONKE
D2DR_HUMAN D(2) DOPAMINE RECEPTOR - HOMO SAPIENS (HUMAN).
D2DR_BOVIN D(2) DOPAMINE RECEPTOR - BOS TAURUS (BOVINE).
D2DR_MOUSE D(2) DOPAMINE RECEPTOR - MUS MUSCULUS (MOUSE), AND RATTUS NO
O73810 D2 DOPAMINE RECEPTOR - MELEAGRIS GALLOPAVO (COMMON TURKEY).
D2D1_XENLA D(2) DOPAMINE RECEPTOR 1 - XENOPUS LAEVIS (AFRICAN CLAWED FR
SCAN HISTORY
OWL28_1 1 50 NSINGLE
SPTR37_9f 2 7 NSINGLE
INITIAL MOTIF SETS
DOPAMINED2R1 Length of motif = 12 Motif number = 1
Dopamine D2 receptor motif I - 1
PCODE ST INT
DPLNLSWYDDDL D2DR_HUMAN 2 2
DPLNLSWYDDDL D2DR_RAT 2 2
DPLNLSWYDDDL CAU18547 2 2
DPLNLSWYDDDL RND2DOPR 2 2
DPLNLSWYDDDP D2DR_BOVIN 2 2
DPQNLSMYNDDI D2D1_XENLA 2 2
DOPAMINED2R2 Length of motif = 21 Motif number = 2
Dopamine D2 receptor motif II - 1
PCODE ST INT
CTHPEDMKLCTVIMKSNGSFP D2DR_HUMAN 244 230
CTHPEDMKLCTVIMKSNGSFP D2DR_RAT 244 230
CTHPEDMKLCTVIMKSNGSFP CAU18547 244 230
CTHPEDMKLCTVIMKSNGSFP RND2DOPR 244 230
CTHPEDMKLCTVIMKSNGSFP D2DR_BOVIN 244 230
CTHPEDVKLCSVFVKSNGSFP D2D1_XENLA 235 221
DOPAMINED2R3 Length of motif = 15 Motif number = 3
Dopamine D2 receptor motif III - 1
PCODE ST INT
EMEMLSSTSPPERTR D2DR_HUMAN 280 15
EMEMLSSTSPPERTR D2DR_RAT 280 15
EMEMLSSTSPPERTR CAU18547 280 15
EMEMLSSTSPPERTR RND2DOPR 280 15
EMEMLSSTSPPERTR D2DR_BOVIN 280 15
EMEMMSSTSPPEKTK D2D1_XENLA 275 19
DOPAMINED2R4 Length of motif = 18 Motif number = 4
Dopamine D2 receptor motif IV - 1
PCODE ST INT
LHSTPDSPAKPEKNGHAK D2DR_HUMAN 315 20
LHSNPDSPAKPEKNGHAK D2DR_RAT 315 20
LHSTPDSPAKPEKNGHAK CAU18547 315 20
LHSNPDSPAKPEKNGHAK RND2DOPR 315 20
LHSTPDSPAKPEKNGHAK D2DR_BOVIN 315 20
LTSPVESPYKAEKNGHPK D2D1_XENLA 314 24
DOPAMINED2R5 Length of motif = 15 Motif number = 5
Dopamine D2 receptor motif V - 1
PCODE ST INT
PKIAKIFEIQTMPNG D2DR_HUMAN 335 2
PRIAKFFEIQTMPNG D2DR_RAT 336 3
PKIAKIFEIQTMPNG CAU18547 335 2
PRIAKFFEIQTMPNG RND2DOPR 336 3
PKIAKIFEIQSMPNG D2DR_BOVIN 336 3
TKPAKVFEIQSMPNG D2D1_XENLA 334 2
DOPAMINED2R6 Length of motif = 15 Motif number = 6
Dopamine D2 receptor motif VI - 1
PCODE ST INT
NIHCDCNIPPVLYSA D2DR_HUMAN 396 46
NIHCDCNIPPVLYSA D2DR_RAT 397 46
NIHCDCNIPPVLYSA CAU18547 396 46
NIHCDCNIPPVLYSA RND2DOPR 397 46
NIHCDCNIPPVLYSA D2DR_BOVIN 397 46
NMHCNCNIPQALYSA D2D1_XENLA 395 46
FINAL MOTIF SETS
DOPAMINED2R1 Length of motif = 12 Motif number = 1
Dopamine D2 receptor motif I - 2
PCODE ST INT
DPLNLSWYDDDL D2DR_CERAE 2 2
DPLNLSWYDDDL D2DR_HUMAN 2 2
DPLNLSWYDDDP D2DR_BOVIN 2 2
DPLNLSWYDDDL D2DR_MOUSE 2 2
DPLNLSWYNTGD O73810 2 2
DPQNLSMYNDDI D2D1_XENLA 2 2
DOPAMINED2R2 Length of motif = 21 Motif number = 2
Dopamine D2 receptor motif II - 2
PCODE ST INT
CTHPEDMKLCTVIMKSNGSFP D2DR_CERAE 244 230
CTHPEDMKLCTVIMKSNGSFP D2DR_HUMAN 244 230
CTHPEDMKLCTVIMKSNGSFP D2DR_BOVIN 244 230
CTHPEDMKLCTVIMKSNGSFP D2DR_MOUSE 244 230
CTHPENVKLGTVIVKSNGSFQ O73810 240 226
CTHPEDVKLCSVFVKSNGSFP D2D1_XENLA 235 221
DOPAMINED2R3 Length of motif = 15 Motif number = 3
Dopamine D2 receptor motif III - 2
PCODE ST INT
EMEMLSSTSPPERTR D2DR_CERAE 280 15
EMEMLSSTSPPERTR D2DR_HUMAN 280 15
EMEMLSSTSPPERTR D2DR_BOVIN 280 15
EMEMLSSTSPPERTR D2DR_MOUSE 280 15
EMEMMSSTSPPERTI O73810 275 14
EMEMMSSTSPPEKTK D2D1_XENLA 275 19
DOPAMINED2R4 Length of motif = 18 Motif number = 4
Dopamine D2 receptor motif IV - 2
PCODE ST INT
LHSTPDSPAKPEKNGHAK D2DR_CERAE 315 20
LHSTPDSPAKPEKNGHAK D2DR_HUMAN 315 20
LHSTPDSPAKPEKNGHAK D2DR_BOVIN 315 20
LHSNPDSPAKPEKNGHAK D2DR_MOUSE 315 20
SRSTLDSPGKVEKNGHAK O73810 307 17
LTSPVESPYKAEKNGHPK D2D1_XENLA 314 24
DOPAMINED2R5 Length of motif = 15 Motif number = 5
Dopamine D2 receptor motif V - 2
PCODE ST INT
PKIAKIFEIQTMPNG D2DR_CERAE 335 2
PKIAKIFEIQTMPNG D2DR_HUMAN 335 2
PKIAKIFEIQSMPNG D2DR_BOVIN 336 3
PRIAKFFEIQTMPNG D2DR_MOUSE 336 3
LHTAKVFEIQSMPNG O73810 327 2
TKPAKVFEIQSMPNG D2D1_XENLA 334 2
DOPAMINED2R6 Length of motif = 15 Motif number = 6
Dopamine D2 receptor motif VI - 2
PCODE ST INT
NIHCDCNIPPVLYSA D2DR_CERAE 396 46
NIHCDCNIPPVLYSA D2DR_HUMAN 396 46
NIHCDCNIPPVLYSA D2DR_BOVIN 397 46
NIHCDCNIPPVLYSA D2DR_MOUSE 397 46
NMHCDCNIPPAMYSA O73810 389 47
NMHCNCNIPQALYSA D2D1_XENLA 395 46
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