WORKLIST ENTRIES (1):
DOPAMINED3R View alignment View Structure Dopamine D3 receptor signature
Type of fingerprint: COMPOUND with 7 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00242 DOPAMINER; PR00565 DOPAMINED1AR; PR00566 DOPAMINED1BR
PRINTS; PR00567 DOPAMINED2R; PR00569 DOPAMINED4R
INTERPRO; IPR001620
Creation date 17-AUG-1996; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. GRANDY, D.K., MARCHIONNI, M.A., MAKAM, H., STOFKO, R.E., ALFANO, M.,
FROTHINGHAM, L., FISCHER, J.B., BURKE-HOWIE, K.J., BUNZOW, J.R.,
SERVER, A.C. AND CIVELLI, O.
Cloning of the cDNA and gene for a human D2 dopamine receptor.
PROC.NATL.ACAD.SCI.U.S.A. 86 9762-9766 (1989).
7. WATSON, S. AND ARKINSTALL, S.
Dopamine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.96-110.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Dopamine neurons in the vertebrate central nervous system are involved in
the initiation and execution of movement, the maintenance of emotional
stability, and the regulation of pituitary function [6]. Various human
neurological diseases (e.g., Parkinson disease and schizophrenia), are
believed to be manifestations of dopamine and dopamine receptor imbalance.
The receptors have been divided into several different subtypes (designated
D1-D5), which may be distinguished by their G protein coupling, ligand
specificity, anatomical distribution and physiological effects.
D3 receptors have a similar pharmacological profile to D2 receptors. They
are expressed predominantly in the limbic area (including the olfactory
tubercle, nucleus accumbens, islands of Calleja and hypothalamus), and they
are present in lower levels in the caudate-putamen and cerebral cortex [7].
The receptors are also found in dopamine cell bodies in the substantia
nigra [7]. The distribution of the receptors is consistent with a role in
cognition and emotional functions; they may thus be the target of anti-
psychotic therapy involving dopamine antagonists [7].
DOPAMINED3R is a 7-element fingerprint that provides a signature for the
D3 family of dopamine receptors. The fingerprint was derived from an initial
alignment of 6 sequences: the motifs were drawn from conserved sections
within either loop or N-terminal regions, focusing on those areas of the
alignment that characterise the D3 receptors but distinguish them from the
rest of the rhodopsin-like superfamily - motif 1 lies at the N-terminus;
motif 2 spans the second cytoplasmic loop, leading into the N-terminus of
TM domain 4; motifs 3-6 span the third cytoplasmic loop; and motif 7 spans
the third external loop, leading into the N-terminus of TM domain 7. A
single iteration on OWL28.1 was required to reach convergence, no further
sequences being identified beyond the starting set. Two partial matches
were also found, A48258 and A55419, both of which are D3 receptor fragments.
An update on SPTR37_9f identified a true set of 5 sequences.
SUMMARY INFORMATION
5 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
7| 5 5 5 5 5 5 5
6| 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0
--+------------------------------------
| 1 2 3 4 5 6 7
True positives..
D3DR_HUMAN Q13167 D3DR_CERAE D3DR_MOUSE
D3DR_RAT
PROTEIN TITLES
D3DR_HUMAN D(3) DOPAMINE RECEPTOR - HOMO SAPIENS (HUMAN).
Q13167 DOPAMINE D3 RECEPTOR - HOMO SAPIENS (HUMAN).
D3DR_CERAE D(3) DOPAMINE RECEPTOR - CERCOPITHECUS AETHIOPS (GREEN MONKE
D3DR_MOUSE D(3) DOPAMINE RECEPTOR - MUS MUSCULUS (MOUSE).
D3DR_RAT D(3) DOPAMINE RECEPTOR - RATTUS NORVEGICUS (RAT).
SCAN HISTORY
OWL28_1 1 50 NSINGLE
SPTR37_9f 2 6 NSINGLE
INITIAL MOTIF SETS
DOPAMINED3R1 Length of motif = 20 Motif number = 1
Dopamine D3 receptor motif I - 1
PCODE ST INT
HLNSTCGAENSTGVNRARPH D3DR_RAT 10 10
HLNSTCGAENSTGVNRARPH RATD3A06 10 10
HINSTCGAENSTGVNRARPH D3DR_MOUSE 10 10
HLNYTCGAENSTGASQARPH D3DR_HUMAN 10 10
HLNYTCGAENSTGASQARPH HSU25441 10 10
HLNYTCGVENSTGASQARPH CAU21307 10 10
DOPAMINED3R2 Length of motif = 20 Motif number = 2
Dopamine D3 receptor motif II - 1
PCODE ST INT
VHYQHGTGQSSCRRVALMIT D3DR_RAT 136 106
VHYEHGTGQSSCRRVALMIT RATD3A06 136 106
VHYQHGTGQSSCRRVALMIT D3DR_MOUSE 136 106
VHYQHGTGQSSCRRVALMIT D3DR_HUMAN 136 106
VHYQHGTGQSSCRRVALMIT HSU25441 135 105
VHYQHGTGQSSCRRVTLMIT CAU21307 136 106
DOPAMINED3R3 Length of motif = 17 Motif number = 3
Dopamine D3 receptor motif III - 1
PCODE ST INT
RILTRQNSQCISIRPGF D3DR_RAT 222 66
RILTRQNSQCISIRPGF RATD3A06 230 74
RILTRQNSQCISIRPGF D3DR_MOUSE 222 66
RILTRQNSQCNSVRPGF D3DR_HUMAN 222 66
RILTRQNSQCNSVRPGF HSU25441 221 66
RILTRQNSQCNSVRPGF CAU21307 222 66
DOPAMINED3R4 Length of motif = 16 Motif number = 4
Dopamine D3 receptor motif IV - 1
PCODE ST INT
HGGLKRYYSICQDTAL D3DR_RAT 296 57
HGGLKRYYSICQDTAL RATD3A06 304 57
HGELKRYYSICQDTAL D3DR_MOUSE 296 57
HLELKRYYSICQDTAL D3DR_HUMAN 249 10
HLELKRYYSICQDTAL HSU25441 248 10
HLELKRYYSICQDTAL CAU21307 249 10
DOPAMINED3R5 Length of motif = 19 Motif number = 5
Dopamine D3 receptor motif V - 1
PCODE ST INT
TRNSLSPTMAPKLSLEVRK D3DR_RAT 328 16
TRNSLSPTMAPKLSLEVRK RATD3A06 336 16
TRNSLSPTMAPKLSLEVRK D3DR_MOUSE 328 16
TRNSLSPTIAPKLSLEVRK D3DR_HUMAN 282 17
TRNSLSPTIAPKLSLEVRK HSU25441 281 17
TRNSLSPTIAPKLSLEVRK CAU21307 282 17
DOPAMINED3R6 Length of motif = 21 Motif number = 6
Dopamine D3 receptor motif VI - 1
PCODE ST INT
LSNGRLSTSLRLGPLQPRGVP D3DR_RAT 347 0
LSNGRLSTSLRLGPLQPRGVP RATD3A06 355 0
LSNGRLSTSLKLGPLQPRGVP D3DR_MOUSE 347 0
LSNGRLSTSLKLGPLQPRGVP D3DR_HUMAN 301 0
LSNGRLSTSLKLGPLQPRGVP HSU25441 300 0
LSNGRLSTSLKLGPLQPRGVP CAU21307 301 0
DOPAMINED3R7 Length of motif = 14 Motif number = 7
Dopamine D3 receptor motif VII - 1
PCODE ST INT
HCQACHVSPELYRA D3DR_RAT 400 32
HCQACHVSPELYRA RATD3A06 408 32
HCQACHVSPELYRA D3DR_MOUSE 400 32
HCQTCHVSPELYSA D3DR_HUMAN 354 32
HCQTCHVSPELYSA HSU25441 353 32
HCQTCHVSPELYSA CAU21307 354 32
FINAL MOTIF SETS
DOPAMINED3R1 Length of motif = 20 Motif number = 1
Dopamine D3 receptor motif I - 2
PCODE ST INT
HLNYTCGAENSTGASQARPH D3DR_HUMAN 10 10
HLNYTCGAENSTGASQARPH Q13167 10 10
HLNYTCGVENSTGASQARPH D3DR_CERAE 10 10
HINSTCGAENSTGVNRARPH D3DR_MOUSE 10 10
HLNSTCGAENSTGVNRARPH D3DR_RAT 10 10
DOPAMINED3R2 Length of motif = 20 Motif number = 2
Dopamine D3 receptor motif II - 2
PCODE ST INT
VHYQHGTGQSSCRRVALMIT D3DR_HUMAN 136 106
VHYQHGTGQSSCRRVALMIT Q13167 135 105
VHYQHGTGQSSCRRVTLMIT D3DR_CERAE 136 106
VHYQHGTGQSSCRRVALMIT D3DR_MOUSE 136 106
VHYQHGTGQSSCRRVALMIT D3DR_RAT 136 106
DOPAMINED3R3 Length of motif = 17 Motif number = 3
Dopamine D3 receptor motif III - 2
PCODE ST INT
RILTRQNSQCNSVRPGF D3DR_HUMAN 222 66
RILTRQNSQCNSVRPGF Q13167 221 66
RILTRQNSQCNSVRPGF D3DR_CERAE 222 66
RILTRQNSQCISIRPGF D3DR_MOUSE 222 66
RILTRQNSQCISIRPGF D3DR_RAT 222 66
DOPAMINED3R4 Length of motif = 16 Motif number = 4
Dopamine D3 receptor motif IV - 2
PCODE ST INT
HLELKRYYSICQDTAL D3DR_HUMAN 249 10
HLELKRYYSICQDTAL Q13167 248 10
HLELKRYYSICQDTAL D3DR_CERAE 249 10
HGELKRYYSICQDTAL D3DR_MOUSE 296 57
HGGLKRYYSICQDTAL D3DR_RAT 296 57
DOPAMINED3R5 Length of motif = 19 Motif number = 5
Dopamine D3 receptor motif V - 2
PCODE ST INT
TRNSLSPTIAPKLSLEVRK D3DR_HUMAN 282 17
TRNSLSPTIAPKLSLEVRK Q13167 281 17
TRNSLSPTIAPKLSLEVRK D3DR_CERAE 282 17
TRNSLSPTMAPKLSLEVRK D3DR_MOUSE 328 16
TRNSLSPTMAPKLSLEVRK D3DR_RAT 328 16
DOPAMINED3R6 Length of motif = 21 Motif number = 6
Dopamine D3 receptor motif VI - 2
PCODE ST INT
LSNGRLSTSLKLGPLQPRGVP D3DR_HUMAN 301 0
LSNGRLSTSLKLGPLQPRGVP Q13167 300 0
LSNGRLSTSLKLGPLQPRGVP D3DR_CERAE 301 0
LSNGRLSTSLKLGPLQPRGVP D3DR_MOUSE 347 0
LSNGRLSTSLRLGPLQPRGVP D3DR_RAT 347 0
DOPAMINED3R7 Length of motif = 14 Motif number = 7
Dopamine D3 receptor motif VII - 2
PCODE ST INT
HCQTCHVSPELYSA D3DR_HUMAN 354 32
HCQTCHVSPELYSA Q13167 353 32
HCQTCHVSPELYSA D3DR_CERAE 354 32
HCQACHVSPELYRA D3DR_MOUSE 400 32
HCQACHVSPELYRA D3DR_RAT 400 32
User query: Display/Full Code "DOPAMINED3R"