WORKLIST ENTRIES (1):
ECACCHANNEL View alignment Epithelial calcium channel (ECAC) family signature
Type of fingerprint: COMPOUND with 7 elements
Links:
PRINTS; PR00167 CACHANNEL; PR01097 TRNSRECEPTRP; PR01766 ECACCHANNEL1
PRINTS; PR01767 ECACCHANNEL2; PR01768 TRPVRECEPTOR; PR01769 VRL2RECEPTOR
Creation date 26-SEP-2002
1. ZHU, X., JIANG, M., PEYTON, M., BOULAY, G., HURST, R., STEFANI, E. AND
BIRNBAUMER, L.
Trp, a novel mammalian gene family essential for agonist-activated
capacitative Ca2+ entry.
CELL 85 661-671 (1996).
2. BOULAY, G., ZHU, X., PEYTON, M., JIANG, M., HURST, R., STEFANI, E. AND
BIRNBAUMER, L.
Cloning and expression of a novel mammalian homolog of Drosophila transient
receptor potential (Trp) involved in calcium entry secondary to activation
of receptors coupled by the Gq class of G protein.
J.BIOL.CHEM. 272 29672-29680 (1997).
3. GUNTHORPE, M.J., BENHAM, C.D., RANDALL, A. AND DAVIS, J.B.
The diversity in the vanilloid (TRPV) receptor family of ion channels.
TRENDS PHARMACOL.SCI. 23(4) 183-191 (2002).
4. HOENDEROP, J.G.J., VAN DER KEMP, A.W.C.M., HARTOG, A., VAN OS, C.H.,
WILLEMS, P.H.G.M. AND BINDELS R.J.M.
The epithelial calcium channel, ECaC, is activated by hyperpolarisation and
regulated by cytosolic calcium.
BIOCHEM.BIOPHYS.RES.COMMUM. 261 488-492 (1999).
5. BARLEY, N.F., HOWARD, A., O'CALLAGHAN, D., LEGON, S. AND WALTERS, J.R.F.
Epithelial calcium transporter expression in human duodenum.
AM.J.PHYSIOL.GASTROINTEST.LIVER PHYSIOL. 280(2) G285-290 (2001).
Transient receptor potential (Trp) and related proteins are thought to be
Ca2+ ion channel subunits that mediate capacitative Ca2+ entry in response
to a range of external and internal cell stimuli. Such Ca2+ entry is thought
to be an essential component of cellular responses to many hormones and
growth factors, and acts to replenish intracellular Ca2+ stores that have
been emptied through the action of inositol triphosphate (IP3) and other
agents. In non-excitable cells (i.e., those that lack voltage-gated Ca2+
channels, such as hepatocytes), this mode of Ca2+ entry is thought to be an
important step in generating the oscillations of intracellular Ca2+
concentration that characterise their response to stimulatory agents [1].
Studies on the visual transduction system in Drosophila led to the molecular
cloning of Trp and of a related protein, Trp-like, which show similarity to
voltage-gated Ca2+ channels in the regions known as S3-S6, including the S5-
S6 linker, which forms the ion-selective channel pore [2]. This provided
evidence that Trp and/or related proteins might form mammalian capacitative
Ca2+ entry channels. The sequences of these proteins have varying lengths
(usually 800-1000 amino acid residues), and hydropathy plots suggest they
have 6 or more transmembrane (TM) domains, flanked by cytosolic N- and C-
termini. In addition, most contain N-terminal ankyrin repeats [2,3].
Following the cloning of the vanilloid receptor (VR1), at least 4 other
related proteins have been identified. Together, these form a distinct
subgroup of the Trp family. Members of the vanilloid receptor family (TRPV)
are activated by a diverse range of stimuli, including heat, protons,
lipids, phorbols, phosphorylation, changes in extracellular osmolarity
and/or pressure, and depletion of intracellular calcium stores [3]. To date,
2 vanilloid receptor-like proteins (VRL-1 and VRL-2) and at least 2
epithelial calcium channels (ECAC) have been reported.
The cloning of ECAC1 (also known as ECAC or CaT2) [4] and ECAC2 (CaT1) [5]
revealed proteins distantly related to VRL. They are highly selective for
calcium and are constitutively active, which gives them an appearance of
calcium-transporter proteins under experimental conditions. They are
important in cellular calcium homeostasis and are tightly regulated by
intracellular and extracellular calcium, which cause channel inactivation
and block respectively [3].
ECACCHANNEL is a 7-element fingerprint that provides a signature for the
epithelial calcium channel (ECAC) family. The fingerprint was derived from
an initial alignment of 10 sequences: the motifs were drawn from virtually
the full alignment length - motifs 1 and 2 encode the N-terminal ankyrin
domain; motifs 3 and 4 also lie in the N-terminal region; motif 5 is found
at the C-terminus of the first TM domain; motif 6 encodes TM domain 3; and
and motif 7 lies at the C-terminus. Two iterations on SPTR40_20f were
required to reach convergence, at which point a true set comprising 11
sequences was identified.
SUMMARY INFORMATION
11 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
7| 11 11 11 11 11 11 11
6| 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0
--+------------------------------------
| 1 2 3 4 5 6 7
True positives..
Q91WD2 Q9H296 Q9H1D1 Q9H1D0
Q8WXR8 Q9JJJ0 Q9R186 Q9NQA5
Q9XSM3 Q9JIP0 Q9JJL2
PROTEIN TITLES
Q91WD2 SIMILAR TO EPITHELIAL APICAL MEMBRANE CALCIUM TRANSPORTER/CH
Q9H296 CALCIUM TRANSPORT PROTEIN CAT1 - Homo sapiens (Human).
Q9H1D1 CAT-LIKE A PROTEIN - Homo sapiens (Human).
Q9H1D0 CAT-LIKE B PROTEIN - Homo sapiens (Human).
Q8WXR8 CALCIUM TRANSPORT PROTEIN CAT1 - Homo sapiens (Human).
Q9JJJ0 CALCIUM TRANSPORTING PROTEIN HOMOLOG - Mus musculus (Mouse).
Q9R186 CALCIUM TRANSPORTER CAT1 - Rattus norvegicus (Rat).
Q9NQA5 EPITHELIAL CALCIUM CHANNEL (CALCIUM TRANSPORT PROTEIN CAT2)
Q9XSM3 EPITHELIAL CALCIUM CHANNEL - Oryctolagus cuniculus (Rabbit).
Q9JIP0 CALCIUM TRANSPORTER CAT2 - Rattus norvegicus (Rat).
Q9JJL2 EPITHELIAL CALCIUM CHANNEL - Rattus norvegicus (Rat).
SCAN HISTORY
SPTR40_20f 2 100 NSINGLE
INITIAL MOTIF SETS
ECACCHANNEL1 Length of motif = 22 Motif number = 1
Epithelial calcium channel (ECAC) family motif I - 1
PCODE ST INT
RGAMGETALHIAALYDNLEAAM Q9H1D0 75 75
RGAMGETALHIAALYDNLEAAM Q9H296 75 75
RGAMGETALHIAALYDNLEAAM Q9H1D1 75 75
RGAMGETALHIAALYDNLEAAM Q91WD2 75 75
KGAMGETALHIAALYDNLEAAM Q9R186 75 75
RGAMGETALHIAALYDNLEAAM Q9JJJ0 75 75
RGALGETALHIAALYDNLEAAL Q9NQA5 75 75
RGAVGETALHVAALYDNLEAAT Q9XSM3 75 75
RGALGETALHVAALYDNLDAAI Q9JIP0 69 69
RGALGETALHVAALYDNLDAAI Q9JJL2 69 69
ECACCHANNEL2 Length of motif = 16 Motif number = 2
Epithelial calcium channel (ECAC) family motif II - 1
PCODE ST INT
VLMEAAPELVFEPMTS Q9H1D0 97 0
VLMEAAPELVFEPMTS Q9H296 97 0
VLMEAAPELVFEPMTS Q9H1D1 97 0
VLMEAAPELVFEPMTS Q91WD2 97 0
VLMEAAPELVFEPMTS Q9R186 97 0
VLMEAAPELVFEPMTS Q9JJJ0 97 0
VLMEAAPELVFEPTTC Q9NQA5 97 0
LLMEAAPELAKEPALC Q9XSM3 97 0
MLMETAPYLVTESTLC Q9JIP0 91 0
MLMETAPYLVTESTLC Q9JJL2 91 0
ECACCHANNEL3 Length of motif = 24 Motif number = 3
Epithelial calcium channel (ECAC) family motif III - 1
PCODE ST INT
HILILQPNKTFACQMYNLLLSYDR Q9H1D0 201 88
HILILQPNKTFACQMYNLLLSYDR Q9H296 201 88
HILILQPNKTFACQMYNLLLSYDR Q9H1D1 201 88
HILILQPNKTFACQMYNLLLSYDG Q91WD2 201 88
HILILQPNKTFACQMYNLLLSYDG Q9R186 201 88
NILILQPNKTFACQMYNLLLSYDG Q9JJJ0 202 89
HILILQPNKTFACQMYNLLLSYDG Q9NQA5 201 88
HILILQPNKTFACQMYNLLLSYDE Q9XSM3 201 88
HILVLQPNKTFACQMYNLLLSHDG Q9JIP0 195 88
HILVLQPNKTFACQMYNLLLSHDG Q9JJL2 195 88
ECACCHANNEL4 Length of motif = 21 Motif number = 4
Epithelial calcium channel (ECAC) family motif IV - 1
PCODE ST INT
GDEQSLLELIITTKKREARQI Q9H1D0 287 62
GDEQSLLELIITTKKREARQI Q9H296 287 62
GDEQSLLELIITTKKREARQI Q9H1D1 287 62
GDDQSLLELIVTTKKREARQI Q91WD2 286 61
GDDQSLLELIVTTKKREARQI Q9R186 286 61
GDDQSLLELIVTTKKREARQI Q9JJJ0 287 61
GEELSFLELVVSSDKREARQI Q9NQA5 287 62
GEELSFLELVVSSKKREARQI Q9XSM3 287 62
GEDLSFLELVVSSKKKEARQI Q9JIP0 280 61
GEDLSFLELVLSSKKIEARQI Q9JJL2 280 61
ECACCHANNEL5 Length of motif = 22 Motif number = 5
Epithelial calcium channel (ECAC) family motif V - 1
PCODE ST INT
IICFTMCCIYRPLKPRTNNRTS Q9H1D0 340 32
IICFTMCCIYRPLKPRTNNRTS Q9H296 340 32
IICFTMCCIYRPLKPRTNNRTS Q9H1D1 340 32
IICFTMCCVYRPLKPRITNRTN Q91WD2 339 32
IICFTMCCVYRPLKPRITNRTN Q9R186 339 32
IICFTMCCVYRPLKPRITNRTN Q9JJJ0 340 32
MICFTTCCVYRPLKFRGGNRTH Q9NQA5 340 32
MICFTTCCIYRPLKLRDDNRTD Q9XSM3 340 32
MICFTTCCVYRPLKFRDANRTH Q9JIP0 333 32
MICFTTCCVYRPLKFRDANRTH Q9JJL2 333 32
ECACCHANNEL6 Length of motif = 27 Motif number = 6
Epithelial calcium channel (ECAC) family motif VI - 1
PCODE ST INT
TILGGPFHVLIITYAFMVLVTMVMRLI Q9H1D0 419 57
TILGGPFHVLIITYAFMVLVTMVMRLI Q9H296 419 57
TILGGPFHVLIITYAFMVLVTMVMRLI Q9H1D1 419 57
TILGGPFHVIIITYAFMVLVTMVMRLT Q91WD2 418 57
TILGGPFHVIIVTYAFMVLVTMVMRLT Q9R186 418 57
TILGGPFHVIIITYAFMVLVTMVMRLT Q9JJJ0 419 57
TILGGPFHVIIITYASLVLVTMVMRLT Q9NQA5 419 57
TILGGPFHVIIITYASLVLLTMVMRLT Q9XSM3 419 57
TVLGGPFHVIIITYASLVLLIMVMRLT Q9JIP0 412 57
TVLGGPFHVIIITYASLVLLIMVMRLT Q9JJL2 412 57
ECACCHANNEL7 Length of motif = 27 Motif number = 7
Epithelial calcium channel (ECAC) family motif VII - 1
PCODE ST INT
WPRSGICGREYGLGDRWFLRVEDRQDL Q9H1D0 613 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q9H296 613 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q9H1D1 613 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q91WD2 612 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q9R186 612 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q9JJJ0 614 168
WPRSGICGCEFGLGDRWFLRVENHNDQ Q9NQA5 613 167
WPRSGICGYEYGLGDRWFLRVENHHDQ Q9XSM3 613 167
WPRSGICGCEYGLGDRWFLRVEHHQEQ Q9JIP0 606 167
WPRSGICGCEYGLGDRWFLRVEHHQEQ Q9JJL2 606 167
FINAL MOTIF SETS
ECACCHANNEL1 Length of motif = 22 Motif number = 1
Epithelial calcium channel (ECAC) family motif I - 2
PCODE ST INT
RGAMGETALHIAALYDNLEAAM Q91WD2 75 75
RGAMGETALHIAALYDNLEAAM Q8WXR8 75 75
RGAMGETALHIAALYDNLEAAM Q9H1D0 75 75
RGAMGETALHIAALYDNLEAAM Q9H1D1 75 75
RGAMGETALHIAALYDNLEAAM Q9H296 75 75
RGAMGETALHIAALYDNLEAAM Q9JJJ0 75 75
KGAMGETALHIAALYDNLEAAM Q9R186 75 75
RGALGETALHIAALYDNLEAAL Q9NQA5 75 75
RGAVGETALHVAALYDNLEAAT Q9XSM3 75 75
RGALGETALHVAALYDNLDAAI Q9JIP0 69 69
RGALGETALHVAALYDNLDAAI Q9JJL2 69 69
ECACCHANNEL2 Length of motif = 16 Motif number = 2
Epithelial calcium channel (ECAC) family motif II - 2
PCODE ST INT
VLMEAAPELVFEPMTS Q91WD2 97 0
VLMEAAPELVFEPMTS Q8WXR8 97 0
VLMEAAPELVFEPMTS Q9H1D0 97 0
VLMEAAPELVFEPMTS Q9H1D1 97 0
VLMEAAPELVFEPMTS Q9H296 97 0
VLMEAAPELVFEPMTS Q9JJJ0 97 0
VLMEAAPELVFEPMTS Q9R186 97 0
VLMEAAPELVFEPTTC Q9NQA5 97 0
LLMEAAPELAKEPALC Q9XSM3 97 0
MLMETAPYLVTESTLC Q9JIP0 91 0
MLMETAPYLVTESTLC Q9JJL2 91 0
ECACCHANNEL3 Length of motif = 24 Motif number = 3
Epithelial calcium channel (ECAC) family motif III - 2
PCODE ST INT
HILILQPNKTFACQMYNLLLSYDG Q91WD2 201 88
HILILQPNKTFACQMYNLLLSYDR Q8WXR8 201 88
HILILQPNKTFACQMYNLLLSYDR Q9H1D0 201 88
HILILQPNKTFACQMYNLLLSYDR Q9H1D1 201 88
HILILQPNKTFACQMYNLLLSYDR Q9H296 201 88
NILILQPNKTFACQMYNLLLSYDG Q9JJJ0 202 89
HILILQPNKTFACQMYNLLLSYDG Q9R186 201 88
HILILQPNKTFACQMYNLLLSYDG Q9NQA5 201 88
HILILQPNKTFACQMYNLLLSYDE Q9XSM3 201 88
HILVLQPNKTFACQMYNLLLSHDG Q9JIP0 195 88
HILVLQPNKTFACQMYNLLLSHDG Q9JJL2 195 88
ECACCHANNEL4 Length of motif = 21 Motif number = 4
Epithelial calcium channel (ECAC) family motif IV - 2
PCODE ST INT
GDDQSLLELIVTTKKREARQI Q91WD2 286 61
GDEQSLLELIITTKKREARQI Q8WXR8 287 62
GDEQSLLELIITTKKREARQI Q9H1D0 287 62
GDEQSLLELIITTKKREARQI Q9H1D1 287 62
GDEQSLLELIITTKKREARQI Q9H296 287 62
GDDQSLLELIVTTKKREARQI Q9JJJ0 287 61
GDDQSLLELIVTTKKREARQI Q9R186 286 61
GEELSFLELVVSSDKREARQI Q9NQA5 287 62
GEELSFLELVVSSKKREARQI Q9XSM3 287 62
GEDLSFLELVVSSKKKEARQI Q9JIP0 280 61
GEDLSFLELVLSSKKIEARQI Q9JJL2 280 61
ECACCHANNEL5 Length of motif = 22 Motif number = 5
Epithelial calcium channel (ECAC) family motif V - 2
PCODE ST INT
IICFTMCCVYRPLKPRITNRTN Q91WD2 339 32
IICFTMCCIYRPLKPRTNNRTS Q8WXR8 340 32
IICFTMCCIYRPLKPRTNNRTS Q9H1D0 340 32
IICFTMCCIYRPLKPRTNNRTS Q9H1D1 340 32
IICFTMCCIYRPLKPRTNNRTS Q9H296 340 32
IICFTMCCVYRPLKPRITNRTN Q9JJJ0 340 32
IICFTMCCVYRPLKPRITNRTN Q9R186 339 32
MICFTTCCVYRPLKFRGGNRTH Q9NQA5 340 32
MICFTTCCIYRPLKLRDDNRTD Q9XSM3 340 32
MICFTTCCVYRPLKFRDANRTH Q9JIP0 333 32
MICFTTCCVYRPLKFRDANRTH Q9JJL2 333 32
ECACCHANNEL6 Length of motif = 27 Motif number = 6
Epithelial calcium channel (ECAC) family motif VI - 2
PCODE ST INT
TILGGPFHVIIITYAFMVLVTMVMRLT Q91WD2 418 57
TILGGPFHVLIITYAFMVLVTMVMRLI Q8WXR8 419 57
TILGGPFHVLIITYAFMVLVTMVMRLI Q9H1D0 419 57
TILGGPFHVLIITYAFMVLVTMVMRLI Q9H1D1 419 57
TILGGPFHVLIITYAFMVLVTMVMRLI Q9H296 419 57
TILGGPFHVIIITYAFMVLVTMVMRLT Q9JJJ0 419 57
TILGGPFHVIIVTYAFMVLVTMVMRLT Q9R186 418 57
TILGGPFHVIIITYASLVLVTMVMRLT Q9NQA5 419 57
TILGGPFHVIIITYASLVLLTMVMRLT Q9XSM3 419 57
TVLGGPFHVIIITYASLVLLIMVMRLT Q9JIP0 412 57
TVLGGPFHVIIITYASLVLLIMVMRLT Q9JJL2 412 57
ECACCHANNEL7 Length of motif = 27 Motif number = 7
Epithelial calcium channel (ECAC) family motif VII - 2
PCODE ST INT
WPRSGICGREYGLGDRWFLRVEDRQDL Q91WD2 612 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q8WXR8 613 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q9H1D0 613 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q9H1D1 613 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q9H296 613 167
WPRSGICGREYGLGDRWFLRVEDRQDL Q9JJJ0 614 168
WPRSGICGREYGLGDRWFLRVEDRQDL Q9R186 612 167
WPRSGICGCEFGLGDRWFLRVENHNDQ Q9NQA5 613 167
WPRSGICGYEYGLGDRWFLRVENHHDQ Q9XSM3 613 167
WPRSGICGCEYGLGDRWFLRVEHHQEQ Q9JIP0 606 167
WPRSGICGCEYGLGDRWFLRVEHHQEQ Q9JJL2 606 167
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