WORKLIST ENTRIES (1):
GLHYDRLASE58 View alignment Glycosyl hydrolase family 58 signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
PRINTS; PR00843 GLHYDRLASE30; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36
PRINTS; PR00744 GLHYDRLASE37; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41
PRINTS; PR00747 GLHYDRLASE47; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52
PRINTS; PR00846 GLHYDRLASE56; PR00850 GLHYDRLASE59; PR00748 MELIBIASE
PRINTS; PR00137 LYSOZYME; PR00684 T4LYSOZYME; PR00749 LYSOZYMEG
PRINTS; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
INTERPRO; IPR001724
Creation date 05-MAR-1998; UPDATE 21-JUN-1999
1. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
2. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
3. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
Nucleotide sequences of the Arb genes, which control beta-glucosidase
utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
coli Bgl operon and evidence for a new beta-glycohydrolase family
including enzymes from eubacteria, archaebacteria and humans.
J.BACTERIOL. 174 765-777 (1992).
5. LONG, G.S., BRYANT, J.M., TAYLOR, P.W., LUZIO, J.P.
Complete nucleotide sequence of the gene encoding bacteriophage E
endosialidase: implications for K1E endosialidase structure and function.
BIOCHEM.J. 309 543-550 (1995).
6. PETTER, J.G. AND VIMR, E.R.
Complete nucleotide sequence of the bacteriophage K1F tail gene encoding
endo-n-acylneuraminidase (endo-N) and comparison to an endo-N homolog in
bacteriophage PK1E.
J.BACTERIOL. 175 4354-4363 (1993).
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 58 includes the endo-N-acetyl-neuraminidases (EC
3.2.1.129).
Bacteriophage E specifically recognises and infects strains of E.coli
that display the alpha-2,8-linked polysialic acid K1 capsule [5,6].
Bacteriophage E endosialidase is thought to be responsible for initial
absorption of the phage to the host bacterium [5]. The native enzyme is
probably a trimer of identical 74kDa subunits. Within the K1E endosialidase
sequence, a central region of 500 amino acids shows 84% identity to K1F
endosialidase [5]. Both enzymes contain two copies of a sialidase sequence
motif common to many bacterial and viral sialidases. These motifs flank
the region of greatest identity between the two endosialidases, suggesting
that this domain is involved in binding and hydrolysis of the polysialic
acid substrate [5].
GLHYDRLASE58 is an 8-element fingerprint that provides a signature for
family 58 glycosyl hydrolases. The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from conserved regions
spanning the C-terminal half of the alignment. Two iterations on OWL30.0
were required to reach convergence, at which point a true set comprising
3 sequences was identified.
An update on SPTR37_9f identified a true set of 3 sequences.
SUMMARY INFORMATION
3 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 3 3 3 3 3 3 3 3
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
ENAN_BPK1E ENAN_BPK1F O80102
PROTEIN TITLES
ENAN_BPK1E ENDO-N-ACETYLNEURAMINIDASE (EC 3.2.1.129) (ENDO-N) (ENDOSIAL
ENAN_BPK1F ENDO-N-ACETYLNEURAMINIDASE (EC 3.2.1.129) (ENDO-N) (ENDOSIAL
O80102 ENDO-N-ACETYLNEURAMINIDASE - BACTERIOPHAGE #D.
SCAN HISTORY
OWL30_0 2 75 NSINGLE
SPTR37_9f 2 20 NSINGLE
INITIAL MOTIF SETS
GLHYDRLASE581 Length of motif = 25 Motif number = 1
Glycosyl hydrolase family 58 motif I - 1
PCODE ST INT
GELYKITDNPYYNAWPQDKAFVYEN ENAN_BPK1E 104 104
GELFKITDTPYYNAWPQDKAFVYEN ENAN_BPK1F 313 313
GLHYDRLASE582 Length of motif = 29 Motif number = 2
Glycosyl hydrolase family 58 motif II - 1
PCODE ST INT
HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1E 169 40
HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1F 378 40
GLHYDRLASE583 Length of motif = 24 Motif number = 3
Glycosyl hydrolase family 58 motif III - 1
PCODE ST INT
GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1E 288 90
GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1F 497 90
GLHYDRLASE584 Length of motif = 27 Motif number = 4
Glycosyl hydrolase family 58 motif IV - 1
PCODE ST INT
RRQIPSEYEPDAAEPCIKYYDGVLYLI ENAN_BPK1E 358 46
RRQIPSEYEPDASEPCIKYYDGVLYLI ENAN_BPK1F 567 46
GLHYDRLASE585 Length of motif = 27 Motif number = 5
Glycosyl hydrolase family 58 motif V - 1
PCODE ST INT
ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1E 436 51
ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1F 645 51
GLHYDRLASE586 Length of motif = 25 Motif number = 6
Glycosyl hydrolase family 58 motif VI - 1
PCODE ST INT
VGVGSVVVKDSFIYYIFGGENHFNP ENAN_BPK1E 492 29
VGVGSVVVKDNYIYYMFGGEDHFNP ENAN_BPK1F 701 29
GLHYDRLASE587 Length of motif = 27 Motif number = 7
Glycosyl hydrolase family 58 motif VII - 1
PCODE ST INT
LKASTSANIRSEMQMEGEYGFIGKSVP ENAN_BPK1E 598 81
IRASTSSNIRSEVLMEGEYGFIGKSIP ENAN_BPK1F 802 76
GLHYDRLASE588 Length of motif = 28 Motif number = 8
Glycosyl hydrolase family 58 motif VIII - 1
PCODE ST INT
GQRLIICGGEGTSSSSGAQITLHGSNSS ENAN_BPK1E 630 5
GQRIIFCGGEGTSSTTGAQITLYGANNT ENAN_BPK1F 834 5
FINAL MOTIF SETS
GLHYDRLASE581 Length of motif = 25 Motif number = 1
Glycosyl hydrolase family 58 motif I - 2
PCODE ST INT
GELYKITDNPYYNAWPQDKAFVYEN ENAN_BPK1E 104 104
GELFKITDTPYYNAWPQDKAFVYEN ENAN_BPK1F 313 313
GSLTKVTDTPFYNAWTQDKTFVYDH O80102 235 235
GLHYDRLASE582 Length of motif = 29 Motif number = 2
Glycosyl hydrolase family 58 motif II - 2
PCODE ST INT
HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1E 169 40
HPDYPTVNYHCMSMGVCRNRLFAMIETRT ENAN_BPK1F 378 40
SVSADRVNYHCMSMGTCGNRLYAVIETRY O80102 306 46
GLHYDRLASE583 Length of motif = 24 Motif number = 3
Glycosyl hydrolase family 58 motif III - 2
PCODE ST INT
GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1E 288 90
GKNWHMGTSFHKSPWRKTDLGLIP ENAN_BPK1F 497 90
GVTWNFATRFWDSPWEITELPEVA O80102 425 90
GLHYDRLASE584 Length of motif = 27 Motif number = 4
Glycosyl hydrolase family 58 motif IV - 2
PCODE ST INT
RRQIPSEYEPDAAEPCIKYYDGVLYLI ENAN_BPK1E 358 46
RRQIPSEYEPDASEPCIKYYDGVLYLI ENAN_BPK1F 567 46
RRTVAQAYADNASEPCVKSYGGVLYLT O80102 504 55
GLHYDRLASE585 Length of motif = 27 Motif number = 5
Glycosyl hydrolase family 58 motif V - 2
PCODE ST INT
ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1E 436 51
ERAENEWEAGAPDDRYKASYPRTFYAR ENAN_BPK1F 645 51
ERAFGEWEGGAPDSRYEGSYPRTFMCK O80102 582 51
GLHYDRLASE586 Length of motif = 25 Motif number = 6
Glycosyl hydrolase family 58 motif VI - 2
PCODE ST INT
VGVGSVVVKDSFIYYIFGGENHFNP ENAN_BPK1E 492 29
VGVGSVVVKDNYIYYMFGGEDHFNP ENAN_BPK1F 701 29
VGVGSVCVKDGWLYYIFGGEDFFSP O80102 640 31
GLHYDRLASE587 Length of motif = 27 Motif number = 7
Glycosyl hydrolase family 58 motif VII - 2
PCODE ST INT
LKASTSANIRSEMQMEGEYGFIGKSVP ENAN_BPK1E 598 81
IRASTSSNIRSEVLMEGEYGFIGKSIP ENAN_BPK1F 802 76
LEYDGTQQSAVRARFDGDYGLISKTVP O80102 744 79
GLHYDRLASE588 Length of motif = 28 Motif number = 8
Glycosyl hydrolase family 58 motif VIII - 2
PCODE ST INT
GQRLIICGGEGTSSSSGAQITLHGSNSS ENAN_BPK1E 630 5
GQRIIFCGGEGTSSTTGAQITLYGANNT ENAN_BPK1F 834 5
RQRLIVSGGDGASSSDGALLQLYGSNHA O80102 776 5
User query: Display/Full Code "GLHYDRLASE58"