WORKLIST ENTRIES (1):
HYALURONDASE View alignment View Structure Bee venom hyaluronidase signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00131 GLHYDRLASE1; PR00132 GLHYDRLASE2; PR00133 GLHYDRLASE3
PRINTS; PR00732 GLHYDRLASE4; PR00733 GLHYDRLASE6; PR00734 GLHYDRLASE7
PRINTS; PR00735 GLHYDRLASE8; PR00134 GLHYDRLASE10; PR00911 GLHYDRLASE11
PRINTS; PR00736 GLHYDRLASE15; PR00737 GLHYDRLASE16; PR00738 GLHYDRLASE20
PRINTS; PR00739 GLHYDRLASE26; PR00740 GLHYDRLASE27; PR00741 GLHYDRLASE29
PRINTS; PR00843 GLHYDRLASE30; PR00742 GLHYDRLASE35; PR00743 GLHYDRLASE36
PRINTS; PR00744 GLHYDRLASE37; PR00745 GLHYDRLASE39; PR00746 GLHYDRLASE41
PRINTS; PR00747 GLHYDRLASE47; PR00844 GLHYDRLASE48; PR00845 GLHYDRLASE52
PRINTS; PR00846 GLHYDRLASE56; PR00849 GLHYDRLASE58; PR00850 GLHYDRLASE59
PRINTS; PR00748 MELIBIASE; PR00137 LYSOZYME; PR00684 T4LYSOZYME
PRINTS; PR00749 LYSOZYMEG; PR00110 ALPHAAMYLASE; PR00750 BETAAMYLASE
PRINTS; PR00841 GLHYDLASE14A; PR00842 GLHYDLASE14B; PR00848 SPERMPH20
INTERPRO; IPR001329
Creation date 14-FEB-1998; UPDATE 07-JUN-1999
1. HENRISSAT, B.
A classification of glycosyl hydrolases based on amino acid sequence
similarities.
BIOCHEM.J. 280 309-316 (1991).
2. HENRISSAT, B. AND BAIROCH, A.
New families in the classification of glycosyl hydrolases based on amino
acid sequence similarities.
BIOCHEM.J. 293 781-788 (1993).
3. HENRISSAT, B. AND BAIROCH, A.
Updating the sequence-based classification of glycosyl hydrolases.
BIOCHEM.J. 316 695-696 (1996).
4. EL HASSOUNI, M., HENRISSAT, B., CHIPPAUX, M. AND BARRAS, F.
Nucleotide sequences of the Arb genes, which control beta-glucosidase
utilisation in Erwinia chrysanthemi - Comparison with the Escherichia
coli Bgl operon and evidence for a new beta-glycohydrolase family
including enzymes from eubacteria, archaebacteria and humans.
J.BACTERIOL. 174 765-777 (1992).
5. GMACHL, M. AND KREIL, G.
Bee venom hyaluronidase is homologous to a membrane protein of mammalian
sperm.
PROC.NATL.ACAD.SCI.U.S.A. 90 3569-3573 (1993).
O-Glycosyl hydrolases (EC 3.2.1.-) are a widespread group of enzymes that
hydrolyse the glycosidic bond between two or more carbohydrates, or between
a carbohydrate and a non-carbohydrate moiety. A classification system for
glycosyl hydrolases, based on sequence similarity, has led to the definition
of up to 60 different families [1-4] (http://expasy.hcuge.ch/cgi-bin/lists?
glycosid.txt). Family 56 encompasses a group of hyaluronidases (EC 3.2.1.35)
that includes venom hyaluronidases [5] and mammalian sperm surface proteins
(PH-20).
The venom of honeybees contains several biologically-active peptides and
two enzymes, one of which is a hyaluronidase [5]. The amino acid sequence
of bee venom hyaluronidase contains 349 amino acids, and includes four
cysteines and a number of potential glycosylation sites [5]. The sequence
shows a high degree of similarity to PH-20, a membrane protein of mammalian
sperm involved in sperm-egg adhesion, supporting the view that hyaluronidases
play a role in fertilisation [5].
HYALURONDASE is an 8-element fingerprint that provides a signature for bee
venom hyaluronidases. The fingerprint was derived from an initial alignment
of 3 sequences: the motifs were drawn from conserved sections spanning
virtually the full alignment length, focusing on those regions that
characterise the venom hyaluronidases but distinguish them from other family
56 glycosyl hydrolases. A single iteration on OWL30.0 was required to reach
convergence, no further sequences being identified beyond the starting set.
A single partial match was also found, a closely related C.elegans sequence
that matches motifs 3 and 4.
An update on SPTR37_9f identified a true set of 3 sequences.
SUMMARY INFORMATION
3 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 3 3 3 3 3 3 3 3
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
HUGA_DOLMA HUGA_VESVU HUGA_APIME
PROTEIN TITLES
HUGA_DOLMA HYALURONOGLUCOSAMINIDASE (EC 3.2.1.35) (HYALURONIDASE) (ALLE
HUGA_VESVU HYALURONOGLUCOSAMINIDASE (EC 3.2.1.35) (HYALURONIDASE) (ALLE
HUGA_APIME HYALURONOGLUCOSAMINIDASE PRECURSOR (EC 3.2.1.35) (HYALURONID
SCAN HISTORY
OWL30_0 1 50 NSINGLE
SPTR37_9f 2 4 NSINGLE
INITIAL MOTIF SETS
HYALURONDASE1 Length of motif = 14 Motif number = 1
Hyaluronoglucosaminidase motif I - 1
PCODE ST INT
FMCHQYGLYFDEVT HUGA_DOLMA 17 17
FMCHQYDLYFDEVT HUGA_VESVU 17 17
FMCHKYGLRFEEVS HUGA_APIME 52 52
HYALURONDASE2 Length of motif = 14 Motif number = 2
Hyaluronoglucosaminidase motif II - 1
PCODE ST INT
ISIFYDPGEFPALL HUGA_DOLMA 47 16
IAIFYDPGEFPALL HUGA_VESVU 47 16
IAILYDPGMFPALL HUGA_APIME 83 17
HYALURONDASE3 Length of motif = 14 Motif number = 3
Hyaluronoglucosaminidase motif III - 1
PCODE ST INT
LDKTYPNRNFNGIG HUGA_DOLMA 91 30
LDKIYPNRNFSGIG HUGA_VESVU 91 30
LINQIPDKSFPGVG HUGA_APIME 127 30
HYALURONDASE4 Length of motif = 16 Motif number = 4
Hyaluronoglucosaminidase motif IV - 1
PCODE ST INT
RNEHPFWDKKMIELEA HUGA_DOLMA 134 29
RNEHPTWNKKMIELEA HUGA_VESVU 134 29
RREHPFWDDQRVEQEA HUGA_APIME 170 29
HYALURONDASE5 Length of motif = 16 Motif number = 5
Hyaluronoglucosaminidase motif V - 1
PCODE ST INT
NMSPNNLVPDCDATAM HUGA_DOLMA 187 37
NMSPNNLVPECDVTAM HUGA_VESVU 187 37
NLTPNQPSAQCEATTM HUGA_APIME 223 37
HYALURONDASE6 Length of motif = 17 Motif number = 6
Hyaluronoglucosaminidase motif VI - 1
PCODE ST INT
RHELTPDQRVGLVQGRV HUGA_DOLMA 225 22
RQELTPDQRIGLVQGRV HUGA_VESVU 225 22
RWNLTSGERVGLVGGRV HUGA_APIME 261 22
HYALURONDASE7 Length of motif = 17 Motif number = 7
Hyaluronoglucosaminidase motif VII - 1
PCODE ST INT
YQDDTNTFLTETDVKKT HUGA_DOLMA 265 23
YQDETNTFLTETDVKKT HUGA_VESVU 265 23
YQDRRDTDLSRADLEAT HUGA_APIME 302 24
HYALURONDASE8 Length of motif = 11 Motif number = 8
Hyaluronoglucosaminidase motif VIII - 1
PCODE ST INT
SSSDVNSLSKC HUGA_DOLMA 298 16
SSSDVNSLSKC HUGA_VESVU 298 16
SSDDINTKAKC HUGA_APIME 335 16
FINAL MOTIF SETS
HYALURONDASE1 Length of motif = 14 Motif number = 1
Hyaluronoglucosaminidase motif I - 2
PCODE ST INT
FMCHQYGLYFDEVT HUGA_DOLMA 17 17
FMCHQYDLYFDEVT HUGA_VESVU 17 17
FMCHKYGLRFEEVS HUGA_APIME 52 52
HYALURONDASE2 Length of motif = 14 Motif number = 2
Hyaluronoglucosaminidase motif II - 2
PCODE ST INT
ISIFYDPGEFPALL HUGA_DOLMA 47 16
IAIFYDPGEFPALL HUGA_VESVU 47 16
IAILYDPGMFPALL HUGA_APIME 83 17
HYALURONDASE3 Length of motif = 14 Motif number = 3
Hyaluronoglucosaminidase motif III - 2
PCODE ST INT
LDKTYPNRNFNGIG HUGA_DOLMA 91 30
LDKIYPNRNFSGIG HUGA_VESVU 91 30
LINQIPDKSFPGVG HUGA_APIME 127 30
HYALURONDASE4 Length of motif = 16 Motif number = 4
Hyaluronoglucosaminidase motif IV - 2
PCODE ST INT
RNEHPFWDKKMIELEA HUGA_DOLMA 134 29
RNEHPTWNKKMIELEA HUGA_VESVU 134 29
RREHPFWDDQRVEQEA HUGA_APIME 170 29
HYALURONDASE5 Length of motif = 16 Motif number = 5
Hyaluronoglucosaminidase motif V - 2
PCODE ST INT
NMSPNNLVPDCDATAM HUGA_DOLMA 187 37
NMSPNNLVPECDVTAM HUGA_VESVU 187 37
NLTPNQPSAQCEATTM HUGA_APIME 223 37
HYALURONDASE6 Length of motif = 17 Motif number = 6
Hyaluronoglucosaminidase motif VI - 2
PCODE ST INT
RHELTPDQRVGLVQGRV HUGA_DOLMA 225 22
RQELTPDQRIGLVQGRV HUGA_VESVU 225 22
RWNLTSGERVGLVGGRV HUGA_APIME 261 22
HYALURONDASE7 Length of motif = 17 Motif number = 7
Hyaluronoglucosaminidase motif VII - 2
PCODE ST INT
YQDDTNTFLTETDVKKT HUGA_DOLMA 265 23
YQDETNTFLTETDVKKT HUGA_VESVU 265 23
YQDRRDTDLSRADLEAT HUGA_APIME 302 24
HYALURONDASE8 Length of motif = 11 Motif number = 8
Hyaluronoglucosaminidase motif VIII - 2
PCODE ST INT
SSSDVNSLSKC HUGA_DOLMA 298 16
SSSDVNSLSKC HUGA_VESVU 298 16
SSDDINTKAKC HUGA_APIME 335 16
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