WORKLIST ENTRIES (1): MTCRUSTACEAN View alignment View Structure Crustacean metallothionein signature Type of fingerprint: COMPOUND with 3 elements Links: PRINTS; PR00859 MTPROKARYOTE; PR00860 MTVERTEBRATE; PR00872 MTDIPTERA PRINTS; PR00873 MTECHINOIDEA; PR00874 MTFUNGIIV; PR00875 MTMOLLUSC PRINTS; PR00876 MTNEMATODE; PR00877 MTPLANTPEC INTERPRO; IPR002045 PFAM; PF00131 metalthio PDB; 1DMC 3Dinfo; 1DME 3Dinfo SCOP; 1DMC; 1DME Creation date 06-MAR-1998; UPDATE 21-JUN-1999 1. KAGI, J.H.R. Overview of Metallothioneins. METHODS ENZYMOL. 205 613-626 (1991). 2. KAGI, J.H.R. AND KOJIMA, Y. Chemistry and biochemistry of metallothionein. EXPERIENTIA SUPPL. 52 25-61 (1987). 3. KAGI, J.H.R. AND SCHAFFER, A. Biochemistry of metallothionein. BIOCHEMISTRY 27 8509-8515 (1988). 4. FOWLER, B.A., HILDEBRAND, C.E., KOJIMA, Y. AND WEBB, M. Nomenclature of metallothionein. EXPERIENTIA SUPPL. 52 21 (1987). 5. KOJIMA, Y. Definitions and nomenclature of metallothioneins. METHODS ENZYMOL. 205 8-10 (1991). 6. BINZ, P.-A. AND KAGI, J.H.R. Molecular evolution of the metallothionein. Suggestions for a natural classification system. IN FOURTH INTERNATIONAL MEETING ON METALLOTHIONEIN, KANSAS CITY (USA), 1997. Metallothioneins (MT) are small proteins that bind heavy metals, such as zinc, copper, cadmium, nickel, etc.. They have a high content of cysteine residues that bind the metal ions through clusters of thiolate bonds [1-3] (http://www.unizh.ch/~mtpage/MT.html). An empirical classification into three classes has been proposed by Fowler et al.  and Kojima . Members of class I are defined to include polypeptides related in the positions of their cysteines to equine MT-1B, and include mammalian MTs as well as MTs from crustaceans and molluscs. Class II groups MTs from a variety of species, including sea urchins, fungi, insects and cyanobacteria. Class III MTs are atypical polypeptides composed of gamma-glutamylcysteinyl units . This original classification system has been found to be limited, in the sense that it does not allow clear differentiation of patterns of structural similarities, either between or within classes. Consequently, all class I and class II MTs (the proteinaceous sequences) have now been grouped into families of phylogenetically-related and thus alignable sequences  (http://www.unizh.ch/~mtpage/classif.html). This system subdivides the MT superfamily into families, subfamilies, subgroups, and isolated isoforms and alleles. The metallothionein superfamily comprises all polypeptides that resemble equine renal metallothionein in several respects : e.g., low molecular weight; high metal content; amino acid composition with high Cys and low aromatic residue content; unique sequence with characteristic distribution of cysteines, and spectroscopic manifestations indicative of metal thiolate clusters. A MT family subsumes MTs that share particular sequence-specific features and are thought to be evolutionarily related. The inclusion of a MT within a family presupposes that its amino acid sequence is alignable with that of all members. Fifteen MT families have been characterised, each family being identified by its number and its taxonomic range: e.g., Family 1: vertebrate MTs. Crustacean MTs belong to family 3; they are 58-60 residue proteins, with 18 totally conserved cysteines. The protein comprises two structural domains (designated alpha and beta), each of which contains 9 cysteines that bind 3 bivalent metal ions. MTCRUSTACEAN is a 3-element fingerprint that provides a signature for family 3 metallothioneins. The fingerprint was derived from an initial alignment of 6 sequences: the motifs were drawn from conserved regions spanning virtually the full alignment length - motif 1 lies in the beta domain and includes 5 metal-binding Cys residues; motif 2 spans the beta and alpha domains, and contains 7 conserved Cys residues; and motif 3, which lies in the alpha domain, encodes a structurally conserved alpha-helix and includes 3 Cys residues. Two iterations on OWL30.0 were required to reach convergence, at which point a true set comprising 8 sequences was identified. An update on SPTR37_9f identified a true set of 8 sequences, and 1 partial match. SUMMARY INFORMATION 8 codes involving 3 elements 1 codes involving 2 elements COMPOSITE FINGERPRINT INDEX 3| 8 8 8 2| 1 0 1 --+---------------- | 1 2 3 True positives.. MT1_SCYSE MT1_CALSI MT2_CALSI MT1_HOMAM MT_CARMA MT_ASTFL MT2_SCYSE MT_POTPO PROTEIN TITLES MT1_SCYSE METALLOTHIONEIN-I (MT-I) - SCYLLA SERRATA (MUD CRAB). MT1_CALSI METALLOTHIONEIN-I (MT-I) (MT-IB/MT-IA) - CALLINECTES SAPIDUS MT2_CALSI METALLOTHIONEIN-II (MT-II) (MT-IIB/MT-IIA) - CALLINECTES SAP MT1_HOMAM METALLOTHIONEIN-1 (CUMT-1) - HOMARUS AMERICANUS (AMERICAN LO MT_CARMA METALLOTHIONEIN (MT) - CARCINUS MAENAS (COMMON SHORE CRAB) ( MT_ASTFL METALLOTHIONEIN (MT) - ASTACUS FLUVIATILIS (BROAD-FINGERED C MT2_SCYSE METALLOTHIONEIN-II (MT-II) - SCYLLA SERRATA (MUD CRAB). MT_POTPO METALLOTHIONEIN (MT) - POTAMON POTAMIOS.
SCAN HISTORY OWL30_0 2 30 NSINGLE SPTR37_9f 2 100 NSINGLE INITIAL MOTIF SETS MTCRUSTACEAN1 Length of motif = 17 Motif number = 1 Crustacean metallothionein motif I - 1 PCODE ST INT PGPCCNDKCVCKEGGCK MT1_SCYSE 1 1 PDPCCNDKCECKEGECK MT2_CALSI 2 2 PDPCCIDKCECKEGGCK MT_CARMA 2 2 PGPCCNDKCVCQEGGCK MT1_CALSI 2 2 PGPCCKDKCECAEGGCK MT1_HOMAM 1 1 PDPCCNDKCDCKEGECK MT2_SCYSE 1 1 MTCRUSTACEAN2 Length of motif = 19 Motif number = 2 Crustacean metallothionein motif I - 1 PCODE ST INT GCQCTSCRCSPCEKCSSGC MT1_SCYSE 19 1 GCKCKSCRCPPCDKCSSEC MT2_CALSI 20 1 GCKCTSCRCTPCEKCSSGC MT_CARMA 20 1 GCQCTSCRCSPCQKCTSGC MT1_CALSI 20 1 GCKCTSCRCAPCEKCTSGC MT1_HOMAM 19 1 GCKCTSCRCPPCEQCSSGC MT2_SCYSE 19 1 MTCRUSTACEAN3 Length of motif = 12 Motif number = 3 Crustacean metallothionein motif III - 1 PCODE ST INT KEECSKTCSKAC MT1_SCYSE 42 4 KEECSKTCSKPC MT2_CALSI 43 4 KEDCCKTCTKPC MT_CARMA 43 4 KEECSKTCTKPC MT1_CALSI 43 4 KDECAKTCSKPC MT1_HOMAM 42 4 KEDCRKTCSKPC MT2_SCYSE 42 4 FINAL MOTIF SETS MTCRUSTACEAN1 Length of motif = 17 Motif number = 1 Crustacean metallothionein motif I - 2 PCODE ST INT PGPCCNDKCVCKEGGCK MT1_SCYSE 1 1 PGPCCNDKCVCQEGGCK MT1_CALSI 2 2 PDPCCNDKCECKEGECK MT2_CALSI 2 2 PGPCCKDKCECAEGGCK MT1_HOMAM 1 1 PDPCCIDKCECKEGGCK MT_CARMA 2 2 PGPCCNDVCECAAGGCK MT_ASTFL 2 2 PDPCCNDKCDCKEGECK MT2_SCYSE 1 1 DPCCAEGTCECEEGKCK MT_POTPO 2 2 MTCRUSTACEAN2 Length of motif = 19 Motif number = 2 Crustacean metallothionein motif II - 2 PCODE ST INT GCQCTSCRCSPCEKCSSGC MT1_SCYSE 19 1 GCQCTSCRCSPCQKCTSGC MT1_CALSI 20 1 GCKCKSCRCPPCDKCSSEC MT2_CALSI 20 1 GCKCTSCRCAPCEKCTSGC MT1_HOMAM 19 1 GCKCTSCRCTPCEKCSSGC MT_CARMA 20 1 GCVCTSCRCSPCDKCTSGC MT_ASTFL 20 1 GCKCTSCRCPPCEQCSSGC MT2_SCYSE 19 1 GCKCTSCRCSPCEKCTSEC MT_POTPO 20 1 MTCRUSTACEAN3 Length of motif = 12 Motif number = 3 Crustacean metallothionein motif III - 2 PCODE ST INT KEECSKTCSKAC MT1_SCYSE 42 4 KEECSKTCTKPC MT1_CALSI 43 4 KEECSKTCSKPC MT2_CALSI 43 4 KDECAKTCSKPC MT1_HOMAM 42 4 KEDCCKTCTKPC MT_CARMA 43 4 KEECAKTCSKPC MT_ASTFL 43 4 KEDCRKTCSKPC MT2_SCYSE 42 4 KEECAKNCTKPC MT_POTPO 43 4
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