WORKLIST ENTRIES (1):
MTFUNGIIV View alignment Fungi-IV metallothionein signature
Type of fingerprint: COMPOUND with 3 elements
Links:
PRINTS; PR00858 MTCRUSTACEAN; PR00859 MTPROKARYOTE; PR00860 MTVERTEBRATE
PRINTS; PR00872 MTDIPTERA; PR00873 MTECHINOIDEA; PR00875 MTMOLLUSC
PRINTS; PR00876 MTNEMATODE; PR00877 MTPLANTPEC
INTERPRO; IPR000869
PROTOMAP; P41927
PFAM; PF00131 metalthio
Creation date 26-JUN-1998; UPDATE 21-JUN-1999
1. KAGI, J.H.R.
Overview of Metallothioneins.
METHODS ENZYMOL. 205 613-626 (1991).
2. KAGI, J.H.R. AND KOJIMA, Y.
Chemistry and biochemistry of metallothionein.
EXPERIENTIA SUPPL. 52 25-61 (1987).
3. KAGI, J.H.R. AND SCHAFFER, A.
Biochemistry of metallothionein.
BIOCHEMISTRY 27 8509-8515 (1988).
4. FOWLER, B.A., HILDEBRAND, C.E., KOJIMA, Y. AND WEBB, M.
Nomenclature of metallothionein.
EXPERIENTIA SUPPL. 52 21 (1987).
5. KOJIMA, Y.
Definitions and nomenclature of metallothioneins.
METHODS ENZYMOL. 205 8-10 (1991).
6. BINZ, P.-A. AND KAGI, J.H.R.
Molecular evolution of the metallothionein. Suggestions for a natural
classification system.
IN FOURTH INTERNATIONAL MEETING ON METALLOTHIONEIN, KANSAS CITY (USA), 1997.
Metallothioneins (MT) are small proteins that bind heavy metals, such as
zinc, copper, cadmium, nickel, etc.. They have a high content of cysteine
residues that bind the metal ions through clusters of thiolate bonds [1-3]
(http://www.unizh.ch/~mtpage/MT.html). An empirical classification into
three classes has been proposed by Fowler et al. [4] and Kojima [5]. Members
of class I are defined to include polypeptides related in the positions of
their cysteines to equine MT-1B, and include mammalian MTs as well as MTs
from crustaceans and molluscs. Class II groups MTs from a variety of
species, including sea urchins, fungi, insects and cyanobacteria. Class
III MTs are atypical polypeptides composed of gamma-glutamylcysteinyl
units [4].
This original classification system has been found to be limited, in the
sense that it does not allow clear differentiation of patterns of structural
similarities, either between or within classes. Consequently, all class I
and class II MTs (the proteinaceous sequences) have now been grouped into
families of phylogenetically-related and thus alignable sequences [6]
(http://www.unizh.ch/~mtpage/classif.html). This system subdivides the MT
superfamily into families, subfamilies, subgroups, and isolated isoforms
and alleles.
The metallothionein superfamily comprises all polypeptides that resemble
equine renal metallothionein in several respects [4]: e.g., low molecular
weight; high metal content; amino acid composition with high Cys and low
aromatic residue content; unique sequence with characteristic distribution
of cysteines, and spectroscopic manifestations indicative of metal thiolate
clusters. A MT family subsumes MTs that share particular sequence-specific
features and are thought to be evolutionarily related. The inclusion of a
MT within a family presupposes that its amino acid sequence is alignable
with that of all members. Fifteen MT families have been characterised, each
family being identified by its number and its taxonomic range: e.g., Family
1: vertebrate MTs.
Fungi-IV (family 11) MTs are proteins of about 55 residues, with 9 conserved
cysteines. In particular, the consensus pattern C-X-K-C-x-C-x(2)-C-K-C has
been found to be diagnostic of family 11 MTs. The protein binds to copper
ions.
MTFUNGIIV is a 3-element fingerprint that provides a signature for fungi-IV
(family 11) metallothioneins. The fingerprint was derived from an initial
alignment of 2 sequences: motif 2 contains 3 metal-binding Cys residues;
and motif 3 includes 6 Cys residues, and spans the characteristic consensus
pattern. A single iteration on OWL30.1 was required to reach convergence,
no further sequences being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 2 sequences.
SUMMARY INFORMATION
2 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
3| 2 2 2
2| 0 0 0
--+----------------
| 1 2 3
True positives..
MT1_YARLI MT2_YARLI
PROTEIN TITLES
MT1_YARLI METALLOTHIONEIN-I - YARROWIA LIPOLYTICA (CANDIDA LIPOLYTICA)
MT2_YARLI METALLOTHIONEIN-II - YARROWIA LIPOLYTICA (CANDIDA LIPOLYTICA
SCAN HISTORY
OWL30_1 1 300 NSINGLE
SPTR37_9f 1 300 NSINGLE
INITIAL MOTIF SETS
MTFUNGIIV1 Length of motif = 20 Motif number = 1
Fungi IV metallothionein motif I - 1
PCODE ST INT
MEFTTAMFGTSLIFTTSTQS MT1_YARLI 1 1
MEFTSALFGASLVQSKHKTT MT2_YARLI 1 1
MTFUNGIIV2 Length of motif = 18 Motif number = 2
Fungi IV metallothionein motif II - 1
PCODE ST INT
KHNLVNNCCCSSSTSESS MT1_YARLI 21 0
KHNLVDSCCCSKPTEKPT MT2_YARLI 22 1
MTFUNGIIV3 Length of motif = 15 Motif number = 3
Fungi IV metallothionein motif III - 1
PCODE ST INT
ASCACTKCGCKTCKC MT1_YARLI 41 2
NSCTCSKCACDSCKC MT2_YARLI 40 0
FINAL MOTIF SETS
MTFUNGIIV1 Length of motif = 20 Motif number = 1
Fungi IV metallothionein motif I - 1
PCODE ST INT
MEFTTAMFGTSLIFTTSTQS MT1_YARLI 1 1
MEFTSALFGASLVQSKHKTT MT2_YARLI 1 1
MTFUNGIIV2 Length of motif = 18 Motif number = 2
Fungi IV metallothionein motif II - 1
PCODE ST INT
KHNLVNNCCCSSSTSESS MT1_YARLI 21 0
KHNLVDSCCCSKPTEKPT MT2_YARLI 22 1
MTFUNGIIV3 Length of motif = 15 Motif number = 3
Fungi IV metallothionein motif III - 1
PCODE ST INT
ASCACTKCGCKTCKC MT1_YARLI 41 2
NSCTCSKCACDSCKC MT2_YARLI 40 0
User query: Display/Full Code "MTFUNGIIV"