WORKLIST ENTRIES (1):
MTPLANTPEC View alignment Plant PEC family metallothionein signature
Type of fingerprint: COMPOUND with 5 elements
Links:
PRINTS; PR00858 MTCRUSTACEAN; PR00859 MTPROKARYOTE; PR00860 MTVERTEBRATE
PRINTS; PR00872 MTDIPTERA; PR00873 MTECHINOIDEA; PR00874 MTFUNGIIV
PRINTS; PR00875 MTMOLLUSC; PR00876 MTNEMATODE
INTERPRO; IPR000316
PROTOMAP; P30570
PFAM; PF00131 metalthio
Creation date 29-JUN-1998; UPDATE 01-JUL-1999
1. KAGI, J.H.R.
Overview of Metallothioneins.
METHODS ENZYMOL. 205 613-626 (1991).
2. KAGI, J.H.R. AND KOJIMA, Y.
Chemistry and biochemistry of metallothionein.
EXPERIENTIA SUPPL. 52 25-61 (1987).
3. KAGI, J.H.R. AND SCHAFFER, A.
Biochemistry of metallothionein.
BIOCHEMISTRY 27 8509-8515 (1988).
4. FOWLER, B.A., HILDEBRAND, C.E., KOJIMA, Y. AND WEBB, M.
Nomenclature of metallothionein.
EXPERIENTIA SUPPL. 52 21 (1987).
5. KOJIMA, Y.
Definitions and nomenclature of metallothioneins.
METHODS ENZYMOL. 205 8-10 (1991).
6. BINZ, P.-A. AND KAGI, J.H.R.
Molecular evolution of the metallothionein. Suggestions for a natural
classification system.
IN FOURTH INTERNATIONAL MEETING ON METALLOTHIONEIN, KANSAS CITY (USA), 1997.
Metallothioneins (MT) are small proteins that bind heavy metals, such as
zinc, copper, cadmium, nickel, etc.. They have a high content of cysteine
residues that bind the metal ions through clusters of thiolate bonds [1-3]
(http://www.unizh.ch/~mtpage/MT.html). An empirical classification into
three classes has been proposed by Fowler et al. [4] and Kojima [5]. Members
of class I are defined to include polypeptides related in the positions of
their cysteines to equine MT-1B, and include mammalian MTs as well as MTs
from crustaceans and molluscs. Class II groups MTs from a variety of
species, including sea urchins, fungi, insects and cyanobacteria. Class
III MTs are atypical polypeptides composed of gamma-glutamylcysteinyl
units [4].
This original classification system has been found to be limited, in the
sense that it does not allow clear differentiation of patterns of structural
similarities, either between or within classes. Consequently, all class I
and class II MTs (the proteinaceous sequences) have now been grouped into
families of phylogenetically-related and thus alignable sequences [6]
(http://www.unizh.ch/~mtpage/classif.html). This system subdivides the MT
superfamily into families, subfamilies, subgroups, and isolated isoforms
and alleles.
The metallothionein superfamily comprises all polypeptides that resemble
equine renal metallothionein in several respects [4]: e.g., low molecular
weight; high metal content; amino acid composition with high Cys and low
aromatic residue content; unique sequence with characteristic distribution
of cysteines, and spectroscopic manifestations indicative of metal thiolate
clusters. A MT family subsumes MTs that share particular sequence-specific
features and are thought to be evolutionarily related. The inclusion of a
MT within a family presupposes that its amino acid sequence is alignable
with that of all members. Fifteen MT families have been characterised, each
family being identified by its number and its taxonomic range: e.g., Family
1: vertebrate MTs.
Plant pec MTs are 76-84 residue proteins, containing 17 conserved cysteines
that bind 5 zinc ions. It is believed that the proteins may have a role in
Zn(2+) homeostasis during embryogenesis.
MTPLANTPEC is a 5-element fingerprint that provides a signature for the
pec family of plant (family 15) metallothioneins. The fingerprint was
derived from an initial alignment of 6 sequences: the motifs were drawn
from conserved sections spanning virtually the full alignment length -
motif 1 contains 3 metal-binding Cys residues; motifs 2-5 include 1, 2, 4
and 3 Cys residues respectively. Two iterations on OWL30.2 were required
to reach convergence, at which point a true set comprising 7 sequences
was identified. Three partial matches were also found: AF004808 and AF010186
are soybean metallothioneins that match motifs 1,3,4 and 1,4 respectively;
and ATTS2731 is a metallothionein fragment from Arabidopsis that matches
motifs 4 and 5.
An update on SPTR37_9f identified a true set of 4 sequences, and 2
partial matches.
SUMMARY INFORMATION
4 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
1 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
5| 4 4 4 4 4
4| 0 0 0 0 0
3| 1 0 1 1 0
2| 1 0 0 1 0
--+--------------------------
| 1 2 3 4 5
True positives..
EC1_WHEAT EC3_WHEAT EC_MAIZE EC1_ARATH
Subfamily: Codes involving 3 elements
Subfamily True positives..
O23958
Subfamily: Codes involving 2 elements
Subfamily True positives..
O22378
PROTEIN TITLES
EC1_WHEAT EC PROTEIN I/II (ZINC-METALLOTHIONEIN CLASS II) - TRITICUM A
EC3_WHEAT EC PROTEIN III (ZINC-METALLOTHIONEIN CLASS II) - TRITICUM AE
EC_MAIZE EC PROTEIN HOMOLOG (ZINC-METALLOTHIONEIN CLASS II) - ZEA MAY
EC1_ARATH EC PROTEIN HOMOLOG - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O23958 METALLOTHIONEIN-II PROTEIN - GLYCINE MAX (SOYBEAN).
O22378 METALLOTHIONEIN-II PROTEIN - GLYCINE MAX (SOYBEAN).
SCAN HISTORY
OWL30_2 2 110 NSINGLE
SPTR37_9f 2 150 NSINGLE
INITIAL MOTIF SETS
MTPLANTPEC1 Length of motif = 9 Motif number = 1
Plant (pec) metallothionein motif I - 1
PCODE ST INT
GCNDSCGCP EC1_ARATH 12 12
SCNDRCGCP EC2_ARATH 11 11
GCNDSCGCP EC3_ARATH 10 10
GCDDKCGCA EC_MAIZE 1 1
GCDDKCGCA EC1_WHEAT 1 1
GCNDKCGCA EC3_WHEAT 1 1
MTPLANTPEC2 Length of motif = 7 Motif number = 2
Plant (pec) metallothionein motif II - 1
PCODE ST INT
PSPCPGG EC1_ARATH 20 -1
PSPCPGG EC2_ARATH 19 -1
PSPCPGG EC3_ARATH 18 -1
AVPCPGG EC_MAIZE 9 -1
AVPCPGG EC1_WHEAT 9 -1
AVPCPGG EC3_WHEAT 9 -1
MTPLANTPEC3 Length of motif = 8 Motif number = 3
Plant (pec) metallothionein motif III - 1
PCODE ST INT
NSCRCRMR EC1_ARATH 27 0
ESCRCKMM EC2_ARATH 26 0
NSCRCRMR EC3_ARATH 25 0
KDCRCTSG EC_MAIZE 16 0
TGCRCTSA EC1_WHEAT 16 0
TGCRCTSA EC3_WHEAT 16 0
MTPLANTPEC4 Length of motif = 8 Motif number = 4
Plant (pec) metallothionein motif IV - 1
PCODE ST INT
CNPCNCPK EC1_ARATH 54 19
CNPCNCPK EC2_ARATH 54 20
CNPCNCPK EC3_ARATH 52 19
CSPCTCGR EC_MAIZE 41 17
CNPCACGR EC1_WHEAT 43 19
CNPCACGR EC3_WHEAT 43 19
MTPLANTPEC5 Length of motif = 10 Motif number = 5
Plant (pec) metallothionein motif V - 1
PCODE ST INT
KTQTQTSAKG EC1_ARATH 61 -1
KTQTQTSAKG EC2_ARATH 61 -1
KTQTQTSDKG EC3_ARATH 59 -1
RATMPSGREN EC_MAIZE 48 -1
REGTPSGRAN EC1_WHEAT 50 -1
REGTPSGRAN EC3_WHEAT 50 -1
FINAL MOTIF SETS
MTPLANTPEC1 Length of motif = 9 Motif number = 1
Plant (pec) metallothionein motif I - 2
PCODE ST INT
GCDDKCGCA EC1_WHEAT 1 1
GCNDKCGCA EC3_WHEAT 1 1
GCDDKCGCA EC_MAIZE 1 1
GCNDSCGCP EC1_ARATH 12 12
MTPLANTPEC2 Length of motif = 7 Motif number = 2
Plant (pec) metallothionein motif II - 2
PCODE ST INT
AVPCPGG EC1_WHEAT 9 -1
AVPCPGG EC3_WHEAT 9 -1
AVPCPGG EC_MAIZE 9 -1
PSPCPGG EC1_ARATH 20 -1
MTPLANTPEC3 Length of motif = 8 Motif number = 3
Plant (pec) metallothionein motif III - 2
PCODE ST INT
TGCRCTSA EC1_WHEAT 16 0
TGCRCTSA EC3_WHEAT 16 0
KDCRCTSG EC_MAIZE 16 0
NSCRCRMR EC1_ARATH 27 0
MTPLANTPEC4 Length of motif = 8 Motif number = 4
Plant (pec) metallothionein motif IV - 2
PCODE ST INT
CNPCACGR EC1_WHEAT 43 19
CNPCACGR EC3_WHEAT 43 19
CSPCTCGR EC_MAIZE 41 17
CNPCNCPK EC1_ARATH 54 19
MTPLANTPEC5 Length of motif = 10 Motif number = 5
Plant (pec) metallothionein motif V - 2
PCODE ST INT
REGTPSGRAN EC1_WHEAT 50 -1
REGTPSGRAN EC3_WHEAT 50 -1
RATMPSGREN EC_MAIZE 48 -1
KTQTQTSAKG EC1_ARATH 61 -1
User query: Display/Full Code "MTPLANTPEC"