WORKLIST ENTRIES (1):
PACAPRECEPTR View alignment Pituitary adenylate cyclase activating polypeptide type 1 receptor signature
Type of fingerprint: COMPOUND with 10 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00491 VASOACTVEIPR; PR01154 VIP1RECEPTOR; PR01155 VIP2RECEPTOR
INTERPRO; IPR002285
Creation date 23-MAY-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.
Molecular cloning and expression of a cDNA encoding the secretin receptor.
EMBO J. 10 1635-1641 (1991).
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H.,
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor.
SCIENCE 254 1022-1024 (1991).
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F.,
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
6. WATSON, S. AND ARKINSTALL, S.
Vasoactive intestinal polypeptide family.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.278-283.
7. WEI, Y. AND MOJSOV, S.
Tissue specific expression of different human receptor types for pituitary
adenylate cyclase activating polypeptide and vasoactive intestinal
polypeptide: implications for their role in human physiology.
J.NEUROENDOCRINOL. 8 811-817 (1996).
8. PISEGNA, J.R. AND WANK, S.A.
Molecular cloning and functional expression of the pituitary adenylate
cyclase-activating polypeptide type I receptor.
PROC.NATL.ACAD.SCI.U.S.A. 90 6345-6349 (1993).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
families: the secretin-like receptors thus bear their own unique '7TM'
signature.
Vasoactive intestinal polypeptide (VIP) has a wide physiological profile.
In the periphery, it induces relaxation in smooth muscle; inhibits
secretion in certain tissues, but stimulates secretion in others; and
modulates activity of cells in the immune system [6]. In the CNS, it has a
range of both excitatory and inhibitory actions. VIP receptors are
distributed widely in the periphery, and occur throughout the gastro-
intestinal tract and genitourinary system, other smooth muscles and
secretory glands. In the CNS, they are found abundantly in, e.g. the cortex,
hippocampus and thalamus [6]. All VIP receptors activate adenylyl cyclase.
There are two structurally distinct receptors that recognise pituitary
adenylate cyclase activating polypeptide (PACAP) and VIP peptides with
similar affinities (PACAP/VIPR-1, PACAP/VIPR-2), as well as a specific
receptor for the PACAP peptide (PACAP-1) [7]. PACAP type I receptors are
present in the hypothalamus and anterior pituitary, where they regulate the
release of adrenocorticotropin, luteinising hormone, growth hormone and
prolactin, and in the adrenal medulla, where they regulate the release of
epinephrine [8]. The receptors are also found in high concentrations in
testicular germ cells, where they may regulate spermatogenesis, and in some
transformed cell lines, such as the rat pancreatic acinar carcinoma cell
AR4-2J [8].
PACAPRECEPTR is a 10-element fingerprint that provides a signature for
pituitary adenylate cyclase activating polypeptide receptors. The
fingerprint was derived from an initial alignment of 4 sequences: the motifs
were drawn from conserved regions spanning the full alignment length,
focusing on those areas that characterise the type 1 PACAP receptors but
distinguish them from the rest of the VIP receptor family - motifs 1-5 lie
in the N-terminal region immediately preceding TM domain 1; motif 6 lies in
the loop between TM domains 2 and 3; motif 7 spans the loop between TM
domains 6 and 7; and motifs 8-10 reside at the C-terminus. A single
iteration on SPTR37_9f was required to reach convergence, no further
sequences being identified beyond the starting set. Two partial matches
were found: O73769 is a goldfish PACAP type 1 receptor that matches motifs
6-10; and VIPR_CARAU is a goldfish type 1 VIP receptor that matches motifs
7 and 9.
SUMMARY INFORMATION
4 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
10| 4 4 4 4 4 4 4 4 4 4
9| 0 0 0 0 0 0 0 0 0 0
8| 0 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 1 1 1 1 1
4| 0 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 1 0 1 0
--+---------------------------------------------------
| 1 2 3 4 5 6 7 8 9 10
True positives..
PACR_MOUSE PACR_RAT PACR_BOVIN PACR_HUMAN
Subfamily: Codes involving 5 elements
Subfamily True positives..
O73769
Subfamily: Codes involving 2 elements
Subfamily True positives..
VIPR_CARAU
PROTEIN TITLES
PACR_MOUSE PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE I RE
PACR_RAT PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE IA R
PACR_BOVIN PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE I RE
PACR_HUMAN PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE I RE
O73769 PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE TYPE 1 RE
VIPR_CARAU VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR (VIP-R) (VIP RECE
SCAN HISTORY
SPTR37_9f 1 50 NSINGLE
INITIAL MOTIF SETS
PACAPRECEPTR1 Length of motif = 22 Motif number = 1
PACAP type 1 receptor motif I - 1
PCODE ST INT
PMAPAMHSDCIFKKEQAMCLEK PACR_HUMAN 16 16
PVAIAMHSDCIFKKEQAMCLER PACR_RAT 15 15
PMAIAMHSDCIFKKEQAMCLER PACR_MOUSE 16 16
PMATAMHSDCIFKKEQAMCLEK PACR_BOVIN 33 33
PACAPRECEPTR2 Length of motif = 18 Motif number = 2
PACAP type 1 receptor motif II - 1
PCODE ST INT
QRANELMGFNDSSPGCPG PACR_HUMAN 39 1
QRANDLMGLNESSPGCPG PACR_RAT 38 1
QRANDLMGLNESSPGCPG PACR_MOUSE 39 1
QRVNDLMGLNDSSPGCPG PACR_BOVIN 56 1
PACAPRECEPTR3 Length of motif = 19 Motif number = 3
PACAP type 1 receptor motif III - 1
PCODE ST INT
ELFRIFNPDQVWETETIGE PACR_HUMAN 79 22
EVFRIFNPDQVWMTETIGD PACR_RAT 78 22
EVFRIFNPDQVWMTETIGD PACR_MOUSE 79 22
ELFRIFNPDQVWETETIGE PACR_BOVIN 96 22
PACAPRECEPTR4 Length of motif = 18 Motif number = 4
PACAP type 1 receptor motif IV - 1
PCODE ST INT
FGDSNSLDLSDMGVVSRN PACR_HUMAN 100 2
FADSNSLEITDMGVVGRN PACR_RAT 99 2
FADSNSLEITDMGVVGRN PACR_MOUSE 100 2
FADSKSLDLSDMRVVSRN PACR_BOVIN 117 2
PACAPRECEPTR5 Length of motif = 20 Motif number = 5
PACAP type 1 receptor motif V - 1
PCODE ST INT
GFDEYESETGDQDYYYLSVK PACR_HUMAN 135 17
GFDDYEPESGDQDYYYLSVK PACR_RAT 134 17
GFDDYEPESGDQDYYYLSVK PACR_MOUSE 135 17
GFEEYESETGDQDYYYLSVK PACR_BOVIN 152 17
PACAPRECEPTR6 Length of motif = 17 Motif number = 6
PACAP type 1 receptor motif VI - 1
PCODE ST INT
YAEQDSNHCFISTVECK PACR_HUMAN 211 56
YAEQDSSHCFVSTVECK PACR_RAT 210 56
YAEQDSSHCFVSTVECK PACR_MOUSE 211 56
YAEQDSNHCFVSTVECK PACR_BOVIN 228 56
PACAPRECEPTR7 Length of motif = 15 Motif number = 7
PACAP type 1 receptor motif VII - 1
PCODE ST INT
FSPENVSKRERLVFE PACR_HUMAN 371 143
FSPENVSKRERLVFE PACR_RAT 426 199
FSPENVSKRERLVFE PACR_MOUSE 399 171
FSPENVSKRERLVFE PACR_BOVIN 416 171
PACAPRECEPTR8 Length of motif = 13 Motif number = 8
PACAP type 1 receptor motif VIII - 1
PCODE ST INT
WRSWKVNRYFAVD PACR_HUMAN 415 29
WRSWKVNRYFTMD PACR_RAT 470 29
WRSWKVNRYFTMD PACR_MOUSE 443 29
WRSWKVNRYFTMD PACR_BOVIN 460 29
PACAPRECEPTR9 Length of motif = 17 Motif number = 9
PACAP type 1 receptor motif IX - 1
PCODE ST INT
HRHPSLASSGVNGGTQL PACR_HUMAN 430 2
HRHPSLASSGVNGGTQL PACR_RAT 485 2
HRHPSLASSGVNGGTQL PACR_MOUSE 458 2
HRHPSLASSGVNGGTQL PACR_BOVIN 475 2
PACAPRECEPTR10 Length of motif = 22 Motif number = 10
PACAP type 1 receptor motif X - 1
PCODE ST INT
LSILSKSSSQIRMSGLPADNLA PACR_HUMAN 446 -1
LSILSKSSSQLRMSSLPADNLA PACR_RAT 501 -1
LSILSKSSSQLRMSSLPADNLA PACR_MOUSE 474 -1
LSILSKSSSQIRMSGLPADNLA PACR_BOVIN 491 -1
FINAL MOTIF SETS
PACAPRECEPTR1 Length of motif = 22 Motif number = 1
PACAP type 1 receptor motif I - 1
PCODE ST INT
PMAIAMHSDCIFKKEQAMCLER PACR_MOUSE 16 16
PVAIAMHSDCIFKKEQAMCLER PACR_RAT 15 15
PMATAMHSDCIFKKEQAMCLEK PACR_BOVIN 33 33
PMAPAMHSDCIFKKEQAMCLEK PACR_HUMAN 16 16
PACAPRECEPTR2 Length of motif = 18 Motif number = 2
PACAP type 1 receptor motif II - 1
PCODE ST INT
QRANDLMGLNESSPGCPG PACR_MOUSE 39 1
QRANDLMGLNESSPGCPG PACR_RAT 38 1
QRVNDLMGLNDSSPGCPG PACR_BOVIN 56 1
QRANELMGFNDSSPGCPG PACR_HUMAN 39 1
PACAPRECEPTR3 Length of motif = 19 Motif number = 3
PACAP type 1 receptor motif III - 1
PCODE ST INT
EVFRIFNPDQVWMTETIGD PACR_MOUSE 79 22
EVFRIFNPDQVWMTETIGD PACR_RAT 78 22
ELFRIFNPDQVWETETIGE PACR_BOVIN 96 22
ELFRIFNPDQVWETETIGE PACR_HUMAN 79 22
PACAPRECEPTR4 Length of motif = 18 Motif number = 4
PACAP type 1 receptor motif IV - 1
PCODE ST INT
FADSNSLEITDMGVVGRN PACR_MOUSE 100 2
FADSNSLEITDMGVVGRN PACR_RAT 99 2
FADSKSLDLSDMRVVSRN PACR_BOVIN 117 2
FGDSNSLDLSDMGVVSRN PACR_HUMAN 100 2
PACAPRECEPTR5 Length of motif = 20 Motif number = 5
PACAP type 1 receptor motif V - 1
PCODE ST INT
GFDDYEPESGDQDYYYLSVK PACR_MOUSE 135 17
GFDDYEPESGDQDYYYLSVK PACR_RAT 134 17
GFEEYESETGDQDYYYLSVK PACR_BOVIN 152 17
GFDEYESETGDQDYYYLSVK PACR_HUMAN 135 17
PACAPRECEPTR6 Length of motif = 17 Motif number = 6
PACAP type 1 receptor motif VI - 1
PCODE ST INT
YAEQDSSHCFVSTVECK PACR_MOUSE 211 56
YAEQDSSHCFVSTVECK PACR_RAT 210 56
YAEQDSNHCFVSTVECK PACR_BOVIN 228 56
YAEQDSNHCFISTVECK PACR_HUMAN 211 56
PACAPRECEPTR7 Length of motif = 15 Motif number = 7
PACAP type 1 receptor motif VII - 1
PCODE ST INT
FSPENVSKRERLVFE PACR_MOUSE 399 171
FSPENVSKRERLVFE PACR_RAT 426 199
FSPENVSKRERLVFE PACR_BOVIN 416 171
FSPENVSKRERLVFE PACR_HUMAN 371 143
PACAPRECEPTR8 Length of motif = 13 Motif number = 8
PACAP type 1 receptor motif VIII - 1
PCODE ST INT
WRSWKVNRYFTMD PACR_MOUSE 443 29
WRSWKVNRYFTMD PACR_RAT 470 29
WRSWKVNRYFTMD PACR_BOVIN 460 29
WRSWKVNRYFAVD PACR_HUMAN 415 29
PACAPRECEPTR9 Length of motif = 17 Motif number = 9
PACAP type 1 receptor motif IX - 1
PCODE ST INT
HRHPSLASSGVNGGTQL PACR_MOUSE 458 2
HRHPSLASSGVNGGTQL PACR_RAT 485 2
HRHPSLASSGVNGGTQL PACR_BOVIN 475 2
HRHPSLASSGVNGGTQL PACR_HUMAN 430 2
PACAPRECEPTR10 Length of motif = 22 Motif number = 10
PACAP type 1 receptor motif X - 1
PCODE ST INT
LSILSKSSSQLRMSSLPADNLA PACR_MOUSE 474 -1
LSILSKSSSQLRMSSLPADNLA PACR_RAT 501 -1
LSILSKSSSQIRMSGLPADNLA PACR_BOVIN 491 -1
LSILSKSSSQIRMSGLPADNLA PACR_HUMAN 446 -1
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