WORKLIST ENTRIES (1):
PHEROMONEBAR View alignment Pheromone B alpha-1 receptor signature
Type of fingerprint: COMPOUND with 12 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00251 BACTRLOPSIN
PRINTS; PR00899 GPCRSTE3; PR00900 PHEROMONEAR
INTERPRO; IPR000481
Creation date 04-APR-1998; UPDATE 22-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. NAKAYAMA, N., MIYAJIMA, A. AND ARAI, K.
Nucleotide sequences of STE2 and STE3, cell type-specific sterile genes
from Saccharomyces cerevisiae.
EMBO J. 4(10) 2643-2648 (1985).
3. BURKHOLDER, A.C. AND HARTWELL, L.H.
The yeast alpha-factor receptor - Structural properties deduced from the
sequence of the STE2 gene.
NUCLEIC ACIDS RES. 13(23) 8463-8475 (1985).
4. MARSH, L. AND HERSKOWITZ, I.
STE2 protein of Saccharomyces kluyveri is a member of the rhodopsin
beta-adrenergic receptor family and is responsible for recognition of
the peptide ligand alpha-factor.
PROC.NATL.ACAD.SCI.U.S.A. 85(11) 3855-3859 (1988).
5. WENDLAND, J. AND KOTHE, E.
Allelic divergence at B alpha 1 pheromone receptor genes of Schizophyllum
commune.
FEMS MICROBIOL.LETT. 145 451-455 (1996).
6. WENDLAND, J., VAILLANCOURT, L.J., HEGNER, J., LENGELER, K.B.,
LADDISON, K.J., SPECHT, C.A., RAPER, C.A. AND KOTHE, E.
THE mating-type locus B alpha 1 of Schizophyllum commune contains a
pheromone receptor gene and putative pheromone genes.
EMBO J. 14 5271-5278 (1995).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
In fungi, the mating of haploid cells is highly regulated. Yeast have a
genetically-determined mating type (a and alpha), mating-type specific
pheromones, pheromone receptors, and a dedicated G protein signalling
pathway that prevents the mating of identical types.
Little is known about the structure and function of the STE2 and STE3
pheromone receptors. It is believed that they are integral membrane proteins
that may be involved in the response to mating factors on the cell membrane
[2-4]. The amino acid sequences of both receptors contain high proportions
of hydrophobic residues grouped into 7 domains, in a manner reminiscent of
the rhodopsins and other receptors believed to interact with G proteins.
However, while a similar 3D framework has been proposed to account for this,
there is no significant sequence similarity either between STE2 and STE3, or
between these and the rhodopsin-type family: the receptors thus bear their
own unique '7TM' signatures.
The STE3 gene in S.cerevisiae is the cell-surface receptor that binds the
13-residue lipopeptide a-factor. Several related fungal pheromone receptor
sequences are known: these include pheromone B alpha 1 and B alpha 3, and
pheromone B beta 1 receptors from Schizophyllum commune; pheromone receptor
1 from Ustilago hordei; and pheromone receptors 1 and 2 from Ustilago maydis.
Members of the family share about 20% sequence identity.
The multiallelic mating type locus B alpha-1 of S.commune encodes
a pheromone receptor and putative pheromone genes [5]. Analysis of this
locus has provided evidence that pheromones and pheromone receptors govern
recognition of self versus non-self, and sexual development in this
homobasidiomycetous fungus [6].
PHEROMONEBAR is a 12-element fingerprint that provides a signature for the
pheromone B alpha-1 receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
spanning the full alignment length, focusing on those regions that
characterise the B alpha-1 receptors but distinguish them from the rest of
the STE3 family - motif 1 spans the N-terminal portion of putative TM domain
1; motif 2 spans the first cytoplasmic loop and the N-terminus of TM domain
2; motif 3 lies in the second cytoplasmic loop; motif 4 lies in the second
external loop; motif 5 spans TM domain 6; motifs 6 and 7 lie in the third
external loop; motif 8 spans the N-terminus of TM domain 7; and motifs 9-12
reside in the C-terminal domain. A single iteration on OWL30.1 was required
to reach convergence, no further sequences being identified beyond the
starting set. A single partial match was found, BAR2_SCHCO, a pheromone B
alpha-2 receptor fragment that matches motifs 1-3.
An update on SPTR37_9f identified a true set of 2 sequences, and 3
partial matches.
SUMMARY INFORMATION
2 codes involving 12 elements
0 codes involving 11 elements
0 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
2 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
12| 2 2 2 2 2 2 2 2 2 2 2 2
11| 0 0 0 0 0 0 0 0 0 0 0 0
10| 0 0 0 0 0 0 0 0 0 0 0 0
9| 0 0 0 0 0 0 0 0 0 0 0 0
8| 0 0 0 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0 0 0 0
5| 1 1 0 0 1 1 1 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0 0 0 0
2| 1 1 1 0 0 0 1 0 0 0 0 0
--+-------------------------------------------------------------
| 1 2 3 4 5 6 7 8 9 10 11 12
True positives..
BAR1_SCHCO BAR3_SCHCO
Subfamily: Codes involving 5 elements
Subfamily True positives..
O74283
Subfamily: Codes involving 2 elements
Subfamily True positives..
O74282
PROTEIN TITLES
BAR1_SCHCO PHEROMONE B ALPHA 1 RECEPTOR - SCHIZOPHYLLUM COMMUNE (BRACKE
BAR3_SCHCO PHEROMONE B ALPHA 3 RECEPTOR - SCHIZOPHYLLUM COMMUNE (BRACKE
O74283 PHEROMONE RECEPTOR - COPRINUS CINEREUS (INKY CAP FUNGUS).
O74282 PHEROMONE RECEPTOR - COPRINUS CINEREUS (INKY CAP FUNGUS).
SCAN HISTORY
OWL30_1 1 100 NSINGLE
SPTR37_9f 1 100 NSINGLE
INITIAL MOTIF SETS
PHEROMONEBAR1 Length of motif = 14 Motif number = 1
Pheromone B alpha receptor motif I - 1
PCODE ST INT
DPLYPLFPIFAFLG BAR1_SCHCO 3 3
DPLYPLFPIFAFLG SVBAR1 3 3
DPTYPLFPTFAFLG SCU76688 3 3
PHEROMONEBAR2 Length of motif = 12 Motif number = 2
Pheromone B alpha receptor motif II - 1
PCODE ST INT
QAWNSGTCFFMM BAR1_SCHCO 29 12
QAWNSGTCFFMM SVBAR1 29 12
QAWNSGTCFFMV SCU76688 29 12
PHEROMONEBAR3 Length of motif = 13 Motif number = 3
Pheromone B alpha receptor motif III - 1
PCODE ST INT
YYIAKVRAVSKTR BAR1_SCHCO 94 53
YYIAKVRAVSKTR SVBAR1 94 53
YHIAKVRAVSHTR SCU76688 94 53
PHEROMONEBAR4 Length of motif = 15 Motif number = 4
Pheromone B alpha receptor motif IV - 1
PCODE ST INT
AVTNTAVNYVVSYVW BAR1_SCHCO 150 43
AVTNTPVTYVVSYVW SVBAR1 150 43
AVFNTPVTYVVSYIW SCU76688 150 43
PHEROMONEBAR5 Length of motif = 17 Motif number = 5
Pheromone B alpha receptor motif V - 1
PCODE ST INT
MALALTEMMCTMPLGIF BAR1_SCHCO 209 44
MALALTEMMCTMPLGIF SVBAR1 209 44
MALAMTEMCCTVPLGIF SCU76688 209 44
PHEROMONEBAR6 Length of motif = 12 Motif number = 6
Pheromone B alpha receptor motif VI - 1
PCODE ST INT
QPWVSLAVTHYG BAR1_SCHCO 237 11
QPWVSLAVTHYG SVBAR1 237 11
EPWVSLKATHYW SCU76688 237 11
PHEROMONEBAR7 Length of motif = 17 Motif number = 7
Pheromone B alpha receptor motif VII - 1
PCODE ST INT
DQVPAIVWRSQHLIVVC BAR1_SCHCO 253 4
DQVPAIVWRSQHLIVVC SVBAR1 253 4
DQYPAVVWRSSHLVVVC SCU76688 253 4
PHEROMONEBAR8 Length of motif = 11 Motif number = 8
Pheromone B alpha receptor motif VIII - 1
PCODE ST INT
CNELTRWCAPV BAR1_SCHCO 269 -1
CNELTRWCAPV SVBAR1 269 -1
CNELTRWLAPV SCU76688 269 -1
PHEROMONEBAR9 Length of motif = 13 Motif number = 9
Pheromone B alpha receptor motif IX - 1
PCODE ST INT
ARRNYAAAWRKVC BAR1_SCHCO 294 14
ARRNYAAAWRRVC SVBAR1 294 14
ARRNYAAAWAWAC SCU76688 294 14
PHEROMONEBAR10 Length of motif = 17 Motif number = 10
Pheromone B alpha receptor motif X - 1
PCODE ST INT
CRALGLPERVPSLPTTK BAR1_SCHCO 306 -1
CRALGLPERVPSLPTTK SVBAR1 306 -1
CRALGLPERIATLPTTK SCU76688 306 -1
PHEROMONEBAR11 Length of motif = 16 Motif number = 11
Pheromone B alpha receptor motif XI - 1
PCODE ST INT
NKGSGFAEKFAAKAKG BAR1_SCHCO 330 7
NKGSGFAEKFAAKAKG SVBAR1 330 7
SKGPGFAEKFAAKAKG SCU76688 323 0
PHEROMONEBAR12 Length of motif = 14 Motif number = 12
Pheromone B alpha receptor motif XII - 1
PCODE ST INT
GFSSFNVKDFTSEL BAR1_SCHCO 345 -1
GFSNFNVKDFTSEF SVBAR1 345 -1
GLSSFNVKDFTSEF SCU76688 338 -1
FINAL MOTIF SETS
PHEROMONEBAR1 Length of motif = 14 Motif number = 1
Pheromone B alpha receptor motif I - 1
PCODE ST INT
DPLYPLFPIFAFLG BAR1_SCHCO 3 3
DPTYPLFPTFAFLG BAR3_SCHCO 3 3
PHEROMONEBAR2 Length of motif = 12 Motif number = 2
Pheromone B alpha receptor motif II - 1
PCODE ST INT
QAWNSGTCFFMM BAR1_SCHCO 29 12
QAWNSGTCFFMV BAR3_SCHCO 29 12
PHEROMONEBAR3 Length of motif = 13 Motif number = 3
Pheromone B alpha receptor motif III - 1
PCODE ST INT
YYIAKVRAVSKTR BAR1_SCHCO 94 53
YHIAKVRAVSHTR BAR3_SCHCO 94 53
PHEROMONEBAR4 Length of motif = 15 Motif number = 4
Pheromone B alpha receptor motif IV - 1
PCODE ST INT
AVTNTPVTYVVSYVW BAR1_SCHCO 150 43
AVFNTPVTYVVSYIW BAR3_SCHCO 150 43
PHEROMONEBAR5 Length of motif = 17 Motif number = 5
Pheromone B alpha receptor motif V - 1
PCODE ST INT
MALALTEMMCTMPLGIF BAR1_SCHCO 209 44
MALAMTEMCCTVPLGIF BAR3_SCHCO 209 44
PHEROMONEBAR6 Length of motif = 12 Motif number = 6
Pheromone B alpha receptor motif VI - 1
PCODE ST INT
QPWVSLAVTHYG BAR1_SCHCO 237 11
EPWVSLKATHYW BAR3_SCHCO 237 11
PHEROMONEBAR7 Length of motif = 17 Motif number = 7
Pheromone B alpha receptor motif VII - 1
PCODE ST INT
DQVPAIVWRSQHLIVVC BAR1_SCHCO 253 4
DQYPAVVWRSSHLVVVC BAR3_SCHCO 253 4
PHEROMONEBAR8 Length of motif = 11 Motif number = 8
Pheromone B alpha receptor motif VIII - 1
PCODE ST INT
CNELTRWCAPV BAR1_SCHCO 269 -1
CNELTRWLAPV BAR3_SCHCO 269 -1
PHEROMONEBAR9 Length of motif = 13 Motif number = 9
Pheromone B alpha receptor motif IX - 1
PCODE ST INT
ARRNYAAAWRRVC BAR1_SCHCO 294 14
ARRNYAAAWAWAC BAR3_SCHCO 294 14
PHEROMONEBAR10 Length of motif = 17 Motif number = 10
Pheromone B alpha receptor motif X - 1
PCODE ST INT
CRALGLPERVPSLPTTK BAR1_SCHCO 306 -1
CRALGLPERIATLPTTK BAR3_SCHCO 306 -1
PHEROMONEBAR11 Length of motif = 16 Motif number = 11
Pheromone B alpha receptor motif XI - 1
PCODE ST INT
NKGSGFAEKFAAKAKG BAR1_SCHCO 330 7
SKGPGFAEKFAAKAKG BAR3_SCHCO 323 0
PHEROMONEBAR12 Length of motif = 14 Motif number = 12
Pheromone B alpha receptor motif XII - 1
PCODE ST INT
GFSNFNVKDFTSEF BAR1_SCHCO 345 -1
GLSSFNVKDFTSEF BAR3_SCHCO 338 -1
User query: Display/Full Code "PHEROMONEBAR"