WORKLIST ENTRIES (1):
PRSTNOIDEP2R View alignment View Structure Prostanoid EP2 receptor signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00428 PROSTAGLNDNR; PR00580 PRSTNOIDEP1R; PR00582 PRSTNOIDEP3R
PRINTS; PR00583 PRSTNOIDE31R; PR00584 PRSTNOIDE32R; PR00585 PRSTNOIDE33R
PRINTS; PR00586 PRSTNOIDEP4R; PR00854 PRSTNOIDDPR; PR00855 PRSTNOIDFPR
PRINTS; PR00856 PRSTNOIDIPR
INTERPRO; IPR001923
Creation date 25-SEP-1996; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Prostanoids.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.239-251.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1,2]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
Prostanoids (prostaglandins (PG) and thromboxanes (TX)) mediate a wide
variety of actions and play important physiological roles in the cardio-
vascular and immune systems, and in pain sensation in peripheral systems
[6]. PGI2 and TXA2 have opposing actions, involving regulation of the
interaction of platelets with the vascular endothelium, while PGE2, PGI2
and PGD2 are powerful vasodilators and potentiate the action of various
autocoids to induce plasma extravasation and pain sensation. To date,
evidence for at least 5 classes of prostanoid receptor has been obtained.
However, identification of subtypes and their distribution is hampered by
expression of more than one receptor within a tissue, coupled with poor
selectivity of available agonists and antagonists.
EP2 receptors mediate vasodilation, bronchodilation and relaxation of
intestinal smooth muscle, and stimulate fluid secretion in the intestine
[6]. The receptors activate adenylate cyclase through Gs [6].
PRSTNOIDEP2R is an 8-element fingerprint that provides a signature for the
prostanoid EP2 receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the prostanoid EP2 receptors but
distinguish them from the rest of the rhodopsin-like superfamily - motifs
1 and 2 span the N-terminus; motif 3 spans the first cytoplasmic loop; motif
4 lies in the first external loop; motifs 5 and 6 lie in the third
cytoplasmic loop; motif 7 spans the third external loop; and motif 8 lies
at the C-terminus. A single iteration on OWL28.0 was required to reach
convergence, no further sequences being identified beyond the starting set.
An update on SPTR37_9f identified a true set of 3 sequences.
SUMMARY INFORMATION
3 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 3 3 3 3 3 3 3 3
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
PE22_MOUSE PE22_RAT PE22_HUMAN
PROTEIN TITLES
PE22_MOUSE PROSTAGLANDIN E2 RECEPTOR, EP2 SUBTYPE (PROSTANOID EP2 RECEP
PE22_RAT PROSTAGLANDIN E2 RECEPTOR, EP2 SUBTYPE (PROSTANOID EP2 RECEP
PE22_HUMAN PROSTAGLANDIN E2 RECEPTOR, EP2 SUBTYPE (PROSTANOID EP2 RECEP
SCAN HISTORY
OWL28_2 1 50 NSINGLE
SPTR37_9f 2 4 NSINGLE
INITIAL MOTIF SETS
PRSTNOIDEP2R1 Length of motif = 13 Motif number = 1
Prostanoid EP2 receptor motif I - 1
PCODE ST INT
EDCKSRQWLLSGE MUSPEREP2S 12 12
EDCETRQWLPPGE PER2_HUMAN 11 11
ENCESRQYLLSDE RNU48858 12 12
PRSTNOIDEP2R2 Length of motif = 13 Motif number = 2
Prostanoid EP2 receptor motif II - 1
PCODE ST INT
ESPAISSVMFSAG MUSPEREP2S 24 -1
ESPAISSVMFSAG PER2_HUMAN 23 -1
ESPAISSVMFTAG RNU48858 24 -1
PRSTNOIDEP2R3 Length of motif = 21 Motif number = 3
Prostanoid EP2 receptor motif III - 1
PCODE ST INT
RRWRGDTGCSAGSRTSISLFH MUSPEREP2S 49 12
RRWRGDVGCSAGRRSSLSLFH PER2_HUMAN 48 12
RRWRGDTGCSAGSRTSISLFH RNU48858 49 12
PRSTNOIDEP2R4 Length of motif = 15 Motif number = 4
Prostanoid EP2 receptor motif IV - 1
PCODE ST INT
RNQTLVALAPESHAC MUSPEREP2S 96 26
RNQTLVALAPESRAC PER2_HUMAN 95 26
RNQTLVALAPESRAC RNU48858 96 26
PRSTNOIDEP2R5 Length of motif = 16 Motif number = 5
Prostanoid EP2 receptor motif V - 1
PCODE ST INT
RSRRSRCGLSGSSLRG MUSPEREP2S 229 118
RSRRSRCGPSLGSGRG PER2_HUMAN 228 118
RSKRSRCGLSGSSLRG RNU48858 229 118
PRSTNOIDEP2R6 Length of motif = 18 Motif number = 6
Prostanoid EP2 receptor motif VI - 1
PCODE ST INT
PGSRRRGERTSMAEETDH MUSPEREP2S 245 0
PGARRRGERVSMAEETDH PER2_HUMAN 245 1
PGSRRRGERTSMAEETDH RNU48858 245 0
PRSTNOIDEP2R7 Length of motif = 19 Motif number = 7
Prostanoid EP2 receptor motif VII - 1
PCODE ST INT
FTIFAYMDETSSLKEKWDL MUSPEREP2S 280 17
FTIFAYMNETSSRKEKWDL PER2_HUMAN 280 17
FTIFAYMDETSSRKEKWDL RNU48858 280 17
PRSTNOIDEP2R8 Length of motif = 18 Motif number = 8
Prostanoid EP2 receptor motif VIII - 1
PCODE ST INT
RLMRSVLCCRTSLRTQEA MUSPEREP2S 324 25
RLMRSVLCCRISLRTQDA PER2_HUMAN 324 25
RLMRSVLCCRTSLRAPEA RNU48858 324 25
FINAL MOTIF SETS
PRSTNOIDEP2R1 Length of motif = 13 Motif number = 1
Prostanoid EP2 receptor motif I - 2
PCODE ST INT
EDCKSRQWLLSGE PE22_MOUSE 12 12
ENCESRQYLLSDE PE22_RAT 12 12
EDCETRQWLPPGE PE22_HUMAN 11 11
PRSTNOIDEP2R2 Length of motif = 13 Motif number = 2
Prostanoid EP2 receptor motif II - 2
PCODE ST INT
ESPAISSVMFSAG PE22_MOUSE 24 -1
ESPAISSVMFTAG PE22_RAT 24 -1
ESPAISSVMFSAG PE22_HUMAN 23 -1
PRSTNOIDEP2R3 Length of motif = 21 Motif number = 3
Prostanoid EP2 receptor motif III - 2
PCODE ST INT
RRWRGDTGCSAGSRTSISLFH PE22_MOUSE 49 12
RRWRGDTGCSAGSRTSISLFH PE22_RAT 49 12
RRWRGDVGCSAGRRSSLSLFH PE22_HUMAN 48 12
PRSTNOIDEP2R4 Length of motif = 15 Motif number = 4
Prostanoid EP2 receptor motif IV - 2
PCODE ST INT
RNQTLVALAPESHAC PE22_MOUSE 96 26
RNQTLVALAPESRAC PE22_RAT 96 26
RNQTLVALAPESRAC PE22_HUMAN 95 26
PRSTNOIDEP2R5 Length of motif = 16 Motif number = 5
Prostanoid EP2 receptor motif V - 2
PCODE ST INT
RSRRSRCGLSGSSLRG PE22_MOUSE 229 118
RSKRSRCGLSGSSLRG PE22_RAT 229 118
RSRRSRCGPSLGSGRG PE22_HUMAN 228 118
PRSTNOIDEP2R6 Length of motif = 18 Motif number = 6
Prostanoid EP2 receptor motif VI - 2
PCODE ST INT
PGSRRRGERTSMAEETDH PE22_MOUSE 245 0
PGSRRRGERTSMAEETDH PE22_RAT 245 0
PGARRRGERVSMAEETDH PE22_HUMAN 245 1
PRSTNOIDEP2R7 Length of motif = 19 Motif number = 7
Prostanoid EP2 receptor motif VII - 2
PCODE ST INT
FTIFAYMDETSSLKEKWDL PE22_MOUSE 280 17
FTIFAYMDETSSRKEKWDL PE22_RAT 280 17
FTIFAYMNETSSRKEKWDL PE22_HUMAN 280 17
PRSTNOIDEP2R8 Length of motif = 18 Motif number = 8
Prostanoid EP2 receptor motif VIII - 2
PCODE ST INT
RLMRSVLCCRTSLRTQEA PE22_MOUSE 324 25
RLMRSVLCCRTSLRAPEA PE22_RAT 324 25
RLMRSVLCCRISLRTQDA PE22_HUMAN 324 25
User query: Display/Full Code "PRSTNOIDEP2R"