WORKLIST ENTRIES (1):
TRPCHANNEL1 View alignment Transient receptor potential channel 1 signature
Type of fingerprint: COMPOUND with 4 elements
Links:
PRINTS; PR01097 TRNSRECEPTRP; PR01643 TRPCHANNEL2; PR01644 TRPCHANNEL3
PRINTS; PR01645 TRPCHANNEL4; PR01646 TRPCHANNEL5; PR01647 TRPCHANNEL6
PRINTS; PR01648 TRPCHANNEL7
MIM; 602343
Creation date 13-NOV-2001
1. ZHU, X., JIANG, M., PEYTON, M., BOULAY, G., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Trp, a novel mammalian gene family essential for agonist-activated
capacitative Ca2+ entry.
CELL 85 661-671 (1996).
2. BOULAY, G., ZHU, X., PEYTON, M., JIANG, M., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Cloning and expression of a novel mammalian homolog of Drosophila transient
receptor potential (Trp) involved in calcium entry secondary to activation
of receptors coupled by the Gq class of G protein.
J.BIOL.CHEM. 272 29672-29680 (1997).
3. CLAPHAM, D., RUNNELS, L. AND STRUBING, C.
The TRP ion channel family.
NAT.REV.NEUROSCI. 2 6387-396 (2001).
4. HARTENECK, C., PLANT, T. AND SCHULTZ, G.
From worm to man: three subfamilies of TRP channels.
TRENDS NEUROSCI. 23 159-166 (2000).
5. WES, P., CHEVESICH, J., JEROMIN, A., ROSENBERG, C., STETTEN, G AND
MONTELL, C.
TRPC1, a human homolog of a Drosophila store-operated channel.
PROC.NATL.ACAD.SCI.U.S.A. 92 9652-9656 (1995).
6. ZITT, C., ZOBEL, A., OBUKHOV, A., HARTENECK, C., KALKBRENNER, F.,
LUCKHOFF, A. AND SCHULTZ, G.
Cloning and functional expression of a human Ca2+-permeable cation channel
activated by calcium store depletion.
NEURON 16 1189-1196 (1996).
7. LINTSCHINGER, B., BALZER-GELDSETZER, M., BASKARAN, T., GRAIER, W.,
ROMANIN, C., ZHU, M. AND GROSCHNER, K.
Coassembly of Trp1 and Trp3 proteins generates diacylglycerol- and Ca2+
-sensitive cation channels.
J.BIOL.CHEM. 275 27799-27805 (2000).
8. STRUBING, C., KRAPIVINSKY, G., KRAPIVINSKY, L. AND CLAPHAM, D.
TRPC1 and TRPC5 form a novel cation channel in mammalian brain.
NEURON 29 645-655 (2001).
Transient receptor potential (Trp) and related proteins are thought to be
Ca2+ ion channel subunits that mediate capacitative Ca2+ entry in response
to a range of external and internal cell stimuli. Such Ca2+ entry is thought
to be an essential component of cellular responses to many hormones and
growth factors, and acts to replenish intracellular Ca2+ stores that have
been emptied through the action of inositol triphosphate (IP3) and other
agents. In non-excitable cells, i.e. those that lack voltage-gated Ca2+
channels, such as hepatocytes, this mode of Ca2+ entry is thought to be an
important step in generating the oscillations of intracellular Ca2+
concentration that characterise their response to stimulatory agents [1].
Studies on the visual transduction system in Drosophila led to the molecular
cloning of Trp and the cDNA of a related protein, Trp-like, which show
similarity to voltage gated Ca2+ channels in the regions known as S3 through
S6, including the S5-S6 linker that forms the ion-selective channel pore [2].
This provided evidence that Trp and/or related proteins might form mammalian
capacitative Ca2+ entry channels.
A number of Trp and Trp-like channel gene isoforms have now been cloned,
including several mammalian homologues. The Trp family is thought to encode
at least 20 Ca2+-permeable channel proteins. Hydropathy analysis suggests
that they share a common transmembrane (TM) topology. Each family member is
predicted to possess 6 TM domains with intracellular N- and C-termini, which
is similar to the core structure of the pore-forming subunits of the voltage
-gated Na+ and Ca2+ channels. By analogy with these proteins, which have
4 linked domains of 6 TM segments, it is likely that Trp channels are
homo- or heterotetramers of 4 single subunits [3].
The Trp family can be divided on the basis of sequence similarity into 3
subfamilies: short (S), long (L) and osm-like (O) Trp channels [4]. The
STrp subfamily includes Drosophila Trp and Trpl-like, and the mammalian
homologues TrpC1-7. Channels of the STrpC subfamily are activated following
receptor-mediated stimulation of different isoforms of phospholipase C [4].
TrpC1 was the first mammalian STrpC subfamily member to be cloned [5]. It
has been shown to function as a store-operated Ca2+-permeable cation channel
in a number of expression systems [1,6]. In mammalian brain, TrpC1 has been
reported to form heteromers with TrpC3 and TrpC5 [7,8]. The TrpC1/TrpC5
heteromers appear to be activated by Gq-linked receptors, but, by contrast
with expression study findings, not by store depletion. This has led to the
proposal that different TrpC heteromers may form diverse receptor-regulated
channels in the mammalian brain [8].
TRPCHANNEL1 is a 4-element fingerprint that provides a signature for
transient receptor potential channel 1. The fingerprint was derived from an
initial alignment of 6 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise TrpC1 but distinguish it from other family
members - motif 1 lies in the intracellular N-terminal region; motifs 2 and
3 reside in the third extracellular loop; and motif 4 lies in the C-terminal
cytoplasmic region. A single iteration on SPTR40_18f was required to reach
convergence, no further sequences being identified beyond the starting set.
SUMMARY INFORMATION
6 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
4| 6 6 6 6
3| 0 0 0 0
2| 0 0 0 0
--+---------------------
| 1 2 3 4
True positives..
TRP1_HUMAN TRP1_RAT TRP1_MOUSE TRP1_RABIT
TRP1_BOVIN Q9PRI8
PROTEIN TITLES
TRP1_HUMAN Short transient receptor potential channel 1 (TrpC1) (TRP-1
TRP1_RAT Short transient receptor potential channel 1 (TrpC1) (TRP-1
TRP1_MOUSE Short transient receptor potential channel 1 (TrpC1) (Transi
TRP1_RABIT Short transient receptor potential channel 1 (TrpC1) (Calciu
TRP1_BOVIN Short transient receptor potential channel 1 (TrpC1) (TRP-1
Q9PRI8 TRANSIENT RECEPTOR POTENTIAL PROTEIN - Xenopus laevis (Afric
SCAN HISTORY
SPTR40_18f 1 300 NSINGLE
INITIAL MOTIF SETS
TRPCHANNEL11 Length of motif = 17 Motif number = 1
Transient receptor potential channel 1 motif I - 1
PCODE ST INT
MALKDVREVKEENTLNE TRP1_RABIT 32 32
MALKDVREVKEENTLNE TRP1_BOVIN 32 32
MALKDVREVKEENTLNE TRP1_RAT 32 32
LALKDVREVKEETTLDE Q9PRI8 17 17
MALKDVREVKEENTLNE TRP1_HUMAN 32 32
MALKDVREVKEENTLNE TRP1_MOUSE 48 48
TRPCHANNEL12 Length of motif = 22 Motif number = 2
Transient receptor potential channel 1 motif II - 1
PCODE ST INT
TPKEQKDCVGIFCEQQSNDTFH TRP1_RABIT 530 481
TPKEQKDCVGIFCEQQSNDTFH TRP1_BOVIN 564 515
TSKEQKDCVGIFCEQQSNDTFH TRP1_RAT 530 481
TTNEQKDCVGIFCEQQSNNTFH Q9PRI8 549 515
TSKEQKDCVGIFCEQQSNDTFH TRP1_HUMAN 564 515
TSKEQKDCVGIFCEQQSNDTFH TRP1_MOUSE 580 515
TRPCHANNEL13 Length of motif = 24 Motif number = 3
Transient receptor potential channel 1 motif III - 1
PCODE ST INT
LFWYIFSLAHVAIFVTRFSYGEEL TRP1_RABIT 560 8
LFWYIFSLAHVAILCTRFSYGEEL TRP1_BOVIN 594 8
LFWYIFSLAHVAIFVTRFSYGEEL TRP1_RAT 560 8
LFWYIFSLAHVTLFVTRFNFSEEL Q9PRI8 579 8
LFWYIFSLAHVAIFVTRFSYGEEL TRP1_HUMAN 594 8
LFWYIFSLAHVAIFVTRFSYGEEL TRP1_MOUSE 610 8
TRPCHANNEL14 Length of motif = 30 Motif number = 4
Transient receptor potential channel 1 motif IV - 1
PCODE ST INT
QNSLKEWRNLKQKRDENYQKVMCCLVHRYL TRP1_RABIT 680 96
QNSLKEWRHLNEKRDENYQKVMCCLVRRYL TRP1_BOVIN 714 96
QNSLKEWRNLKQKRDENYQKVMCCLVHRYL TRP1_RAT 680 96
QNSFKEWKSLRQKRDENYQKVMCCLVHRYL Q9PRI8 699 96
QNSLKEWRNLKQKRDENYQKVMCCLVHRYL TRP1_HUMAN 714 96
QNSLKEWRNLKQKRDENYQKVMCCLVHRYL TRP1_MOUSE 730 96
FINAL MOTIF SETS
TRPCHANNEL11 Length of motif = 17 Motif number = 1
Transient receptor potential channel 1 motif I - 1
PCODE ST INT
MALKDVREVKEENTLNE TRP1_HUMAN 32 32
MALKDVREVKEENTLNE TRP1_RAT 32 32
MALKDVREVKEENTLNE TRP1_MOUSE 48 48
MALKDVREVKEENTLNE TRP1_RABIT 32 32
MALKDVREVKEENTLNE TRP1_BOVIN 32 32
LALKDVREVKEETTLDE Q9PRI8 17 17
TRPCHANNEL12 Length of motif = 22 Motif number = 2
Transient receptor potential channel 1 motif II - 1
PCODE ST INT
TSKEQKDCVGIFCEQQSNDTFH TRP1_HUMAN 564 515
TSKEQKDCVGIFCEQQSNDTFH TRP1_RAT 530 481
TSKEQKDCVGIFCEQQSNDTFH TRP1_MOUSE 580 515
TPKEQKDCVGIFCEQQSNDTFH TRP1_RABIT 530 481
TPKEQKDCVGIFCEQQSNDTFH TRP1_BOVIN 564 515
TTNEQKDCVGIFCEQQSNNTFH Q9PRI8 549 515
TRPCHANNEL13 Length of motif = 24 Motif number = 3
Transient receptor potential channel 1 motif III - 1
PCODE ST INT
LFWYIFSLAHVAIFVTRFSYGEEL TRP1_HUMAN 594 8
LFWYIFSLAHVAIFVTRFSYGEEL TRP1_RAT 560 8
LFWYIFSLAHVAIFVTRFSYGEEL TRP1_MOUSE 610 8
LFWYIFSLAHVAIFVTRFSYGEEL TRP1_RABIT 560 8
LFWYIFSLAHVAILCTRFSYGEEL TRP1_BOVIN 594 8
LFWYIFSLAHVTLFVTRFNFSEEL Q9PRI8 579 8
TRPCHANNEL14 Length of motif = 30 Motif number = 4
Transient receptor potential channel 1 motif IV - 1
PCODE ST INT
QNSLKEWRNLKQKRDENYQKVMCCLVHRYL TRP1_HUMAN 714 96
QNSLKEWRNLKQKRDENYQKVMCCLVHRYL TRP1_RAT 680 96
QNSLKEWRNLKQKRDENYQKVMCCLVHRYL TRP1_MOUSE 730 96
QNSLKEWRNLKQKRDENYQKVMCCLVHRYL TRP1_RABIT 680 96
QNSLKEWRHLNEKRDENYQKVMCCLVRRYL TRP1_BOVIN 714 96
QNSFKEWKSLRQKRDENYQKVMCCLVHRYL Q9PRI8 699 96
User query: Display/Full Code "TRPCHANNEL1"