WORKLIST ENTRIES (1):
TRPCHANNEL2 View alignment Transient receptor potential channel 2 signature
Type of fingerprint: COMPOUND with 7 elements
Links:
PRINTS; PR01097 TRNSRECEPTRP; PR01642 TRPCHANNEL1; PR01644 TRPCHANNEL3
PRINTS; PR01645 TRPCHANNEL4; PR01646 TRPCHANNEL5; PR01647 TRPCHANNEL6
PRINTS; PR01648 TRPCHANNEL7
Creation date 13-NOV-2001
1. ZHU, X., JIANG, M., PEYTON, M., BOULAY, G., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Trp, a novel mammalian gene family essential for agonist-activated
capacitative Ca2+ entry.
CELL 85 661-671 (1996).
2. BOULAY, G., ZHU, X., PEYTON, M., JIANG, M., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Cloning and expression of a novel mammalian homolog of Drosophila transient
receptor potential (Trp) involved in calcium entry secondary to activation
of receptors coupled by the Gq class of G protein.
J.BIOL.CHEM. 272 29672-29680 (1997).
3. CLAPHAM, D., RUNNELS, L. AND STRUBING, C.
The TRP ion channel family.
NAT.REV.NEUROSCI. 2 6387-396 (2001).
4. HARTENECK, C., PLANT, T. AND SCHULTZ, G.
From worm to man: three subfamilies of TRP channels.
TRENDS NEUROSCI. 23 159-166 (2000).
5. VANNIER, B., PEYTON, M., BOULAY, G., BROWN, D., QIN, N., JIANG, M., ZHU,
X. AND BIRNBAUMER, L.
Mouse trp2, the homologue of the human trpc2 pseudogene, encodes mTrp2, a
store depletion-activated capacitative Ca2+ entry channel.
PROC.NATL.ACAD.SCI.U.S.A. 96 2060-2064 (1999).
6.JUNGNICKEL, M., MARRERO, H., BIRNBAUMER, L., LEMOS, J. AND FLORMAN, H.
Trp2 regulates entry of Ca2+ into mouse sperm triggered by egg ZP3.
NAT.CELL BIOL. 3 499-502 (2001).
Transient receptor potential (Trp) and related proteins are thought to be
Ca2+ ion channel subunits that mediate capacitative Ca2+ entry in response
to a range of external and internal cell stimuli. Such Ca2+ entry is thought
to be an essential component of cellular responses to many hormones and
growth factors, and acts to replenish intracellular Ca2+ stores that have
been emptied through the action of inositol triphosphate (IP3) and other
agents. In non-excitable cells, i.e. those that lack voltage-gated Ca2+
channels, such as hepatocytes, this mode of Ca2+ entry is thought to be an
important step in generating the oscillations of intracellular Ca2+
concentration that characterise their response to stimulatory agents [1].
Studies on the visual transduction system in Drosophila led to the molecular
cloning of Trp and the cDNA of a related protein, Trp-like, which show
similarity to voltage gated Ca2+ channels in the regions known as S3 through
S6, including the S5-S6 linker that forms the ion-selective channel pore [2].
This provided evidence that Trp and/or related proteins might form mammalian
capacitative Ca2+ entry channels.
A number of Trp and Trp-like channel gene isoforms have now been cloned,
including several mammalian homologues. The Trp family is thought to encode
at least 20 Ca2+-permeable channel proteins. Hydropathy analysis suggests
that they share a common transmembrane (TM) topology. Each family member is
predicted to possess 6 TM domains with intracellular N- and C-termini, which
is similar to the core structure of the pore-forming subunits of the voltage
-gated Na+ and Ca2+ channels. By analogy with these proteins, which have
4 linked domains of 6 TM segments, it is likely that Trp channels are
homo- or heterotetramers of 4 single subunits [3].
The Trp family can be divided on the basis of sequence similarity into 3
subfamilies: short (S), long (L) and osm-like (O) Trp channels [4]. The
STrp subfamily includes Drosophila Trp and Trpl-like, and the mammalian
homologues TrpC1-7. Channels of the STrpC subfamily are activated following
receptor-mediated stimulation of different isoforms of phospholipase C (PLC)
[4].
TrpC2 was originally cloned from mouse tissue [5]. Rat and bovine isoforms
have also been identified, but the human form appears to be a pseudogene.
Expression studies have demonstrated that TrpC2 may be activated by receptor-
mediated stimulation of PLC, and also by intracellular Ca2+ store depletion
[5]. TrpC2 has been implicated in sperm-egg interactions, having recently
been shown to be essential for the activation of sustained Ca2+ influx into
sperm during fertilisation [6].
TRPCHANNEL2 is a 7-element fingerprint that provides a signature for
transient receptor potential channel 2. The fingerprint was derived from an
initial alignment of 3 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, focusing on those
sections that characterise TrpC2 but distinguish it from other family
members - motif 1 encodes the second extracellular loop and part of TM
domain 4; and motifs 2-5 lie in the the C-terminal cytoplasmic region. A
single iteration on SPTR40_18f was required to reach convergence, no further
sequences being identified beyond the starting set.
SUMMARY INFORMATION
3 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
7| 3 3 3 3 3 3 3
6| 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0
--+------------------------------------
| 1 2 3 4 5 6 7
True positives..
TRP2_RAT TRP2_MOUSE TRP2_BOVIN
PROTEIN TITLES
TRP2_RAT Short transient receptor potential channel 2 (TrpC2) (rTRP2)
TRP2_MOUSE Short transient receptor potential channel 2 (TrpC2) (mTrp2)
TRP2_BOVIN Short transient receptor potential channel 2 homolog (TrpC2)
SCAN HISTORY
SPTR40_18f 1 150 NSINGLE
INITIAL MOTIF SETS
TRPCHANNEL21 Length of motif = 28 Motif number = 1
Transient receptor potential channel 2 motif I - 1
PCODE ST INT
SDSTTCRCFTTAERSEWRTEDPQFLAEV TRP2_MOUSE 767 767
PDGAACHYFTSAERSEWRTEDPQFLAEV TRP2_BOVIN 29 29
SDSSTCRYFTTAERSEWRTEDPQFLAEV TRP2_RAT 483 483
TRPCHANNEL22 Length of motif = 19 Motif number = 2
Transient receptor potential channel 2 motif II - 1
PCODE ST INT
PKAAFYLVRRIFRFLCCGS TRP2_MOUSE 963 168
PKAIFYLLRRVFRFICCCH TRP2_BOVIN 225 168
PKAAFYLLRRIFRFICCGS TRP2_RAT 679 168
TRPCHANNEL23 Length of motif = 23 Motif number = 3
Transient receptor potential channel 2 motif III - 1
PCODE ST INT
CCKAKKSDYPPIGTFTNPGARAG TRP2_MOUSE 983 1
CCKTKKPDYPPIPTFANPGAGAG TRP2_BOVIN 245 1
CCKAKKSDYPPIPTFTNPGARAG TRP2_RAT 699 1
TRPCHANNEL24 Length of motif = 27 Motif number = 4
Transient receptor potential channel 2 motif IV - 1
PCODE ST INT
SYRLRVIKALVQRYIETARREFEETRR TRP2_MOUSE 1014 8
SYRLRVIKALVQRYIETAQREFEETRR TRP2_BOVIN 275 7
SYRLRVIKALVQRYIETARREFEETRR TRP2_RAT 729 7
TRPCHANNEL25 Length of motif = 26 Motif number = 5
Transient receptor potential channel 2 motif V - 1
PCODE ST INT
KDLGNRLTELTKTVSRLQSEVASVQK TRP2_MOUSE 1041 0
KDLGNRLTELTKTVSRLQSEVAGVQR TRP2_BOVIN 302 0
KDLGNRLTELTKTVSRLQSEVASVQK TRP2_RAT 756 0
TRPCHANNEL26 Length of motif = 26 Motif number = 6
Transient receptor potential channel 2 motif VI - 1
PCODE ST INT
RPPDGASILSRYITRVRNSFQNLGPP TRP2_MOUSE 1075 8
RPPGGASVLSRYITRVRNSFQNLGPP TRP2_BOVIN 336 8
RPPDGASILSRYITRVRNSFQNLGPP TRP2_RAT 790 8
TRPCHANNEL27 Length of motif = 23 Motif number = 7
Transient receptor potential channel 2 motif VII - 1
PCODE ST INT
LVHREQEAEGSGDLLLEGDLETK TRP2_MOUSE 1147 46
LVHREQESEGAGDLPQEADLGAK TRP2_BOVIN 407 45
LVHREQEAEGAGDLPLGEDLETK TRP2_RAT 862 46
FINAL MOTIF SETS
TRPCHANNEL21 Length of motif = 28 Motif number = 1
Transient receptor potential channel 2 motif I - 1
PCODE ST INT
SDSSTCRYFTTAERSEWRTEDPQFLAEV TRP2_RAT 483 483
SDSTTCRCFTTAERSEWRTEDPQFLAEV TRP2_MOUSE 767 767
PDGAACHYFTSAERSEWRTEDPQFLAEV TRP2_BOVIN 29 29
TRPCHANNEL22 Length of motif = 19 Motif number = 2
Transient receptor potential channel 2 motif II - 1
PCODE ST INT
PKAAFYLLRRIFRFICCGS TRP2_RAT 679 168
PKAAFYLVRRIFRFLCCGS TRP2_MOUSE 963 168
PKAIFYLLRRVFRFICCCH TRP2_BOVIN 225 168
TRPCHANNEL23 Length of motif = 23 Motif number = 3
Transient receptor potential channel 2 motif III - 1
PCODE ST INT
CCKAKKSDYPPIPTFTNPGARAG TRP2_RAT 699 1
CCKAKKSDYPPIGTFTNPGARAG TRP2_MOUSE 983 1
CCKTKKPDYPPIPTFANPGAGAG TRP2_BOVIN 245 1
TRPCHANNEL24 Length of motif = 27 Motif number = 4
Transient receptor potential channel 2 motif IV - 1
PCODE ST INT
SYRLRVIKALVQRYIETARREFEETRR TRP2_RAT 729 7
SYRLRVIKALVQRYIETARREFEETRR TRP2_MOUSE 1014 8
SYRLRVIKALVQRYIETAQREFEETRR TRP2_BOVIN 275 7
TRPCHANNEL25 Length of motif = 26 Motif number = 5
Transient receptor potential channel 2 motif V - 1
PCODE ST INT
KDLGNRLTELTKTVSRLQSEVASVQK TRP2_RAT 756 0
KDLGNRLTELTKTVSRLQSEVASVQK TRP2_MOUSE 1041 0
KDLGNRLTELTKTVSRLQSEVAGVQR TRP2_BOVIN 302 0
TRPCHANNEL26 Length of motif = 26 Motif number = 6
Transient receptor potential channel 2 motif VI - 1
PCODE ST INT
RPPDGASILSRYITRVRNSFQNLGPP TRP2_RAT 790 8
RPPDGASILSRYITRVRNSFQNLGPP TRP2_MOUSE 1075 8
RPPGGASVLSRYITRVRNSFQNLGPP TRP2_BOVIN 336 8
TRPCHANNEL27 Length of motif = 23 Motif number = 7
Transient receptor potential channel 2 motif VII - 1
PCODE ST INT
LVHREQEAEGAGDLPLGEDLETK TRP2_RAT 862 46
LVHREQEAEGSGDLLLEGDLETK TRP2_MOUSE 1147 46
LVHREQESEGAGDLPQEADLGAK TRP2_BOVIN 407 45
User query: Display/Full Code "TRPCHANNEL2"