WORKLIST ENTRIES (1):
TRPCHANNEL3 View alignment Transient receptor potential channel 3 signature
Type of fingerprint: COMPOUND with 5 elements
Links:
PRINTS; PR01097 TRNSRECEPTRP; PR01642 TRPCHANNEL1; PR01643 TRPCHANNEL2
PRINTS; PR01645 TRPCHANNEL4; PR01646 TRPCHANNEL5; PR01647 TRPCHANNEL6
PRINTS; PR01648 TRPCHANNEL7
MIM; 602345
Creation date 13-NOV-2001
1. ZHU, X., JIANG, M., PEYTON, M., BOULAY, G., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Trp, a novel mammalian gene family essential for agonist-activated
capacitative Ca2+ entry.
CELL 85 661-671 (1996).
2. BOULAY, G., ZHU, X., PEYTON, M., JIANG, M., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Cloning and expression of a novel mammalian homolog of Drosophila transient
receptor potential (Trp) involved in calcium entry secondary to activation
of receptors coupled by the Gq class of G protein.
J.BIOL.CHEM. 272 29672-29680 (1997).
3. CLAPHAM, D., RUNNELS, L. AND STRUBING, C.
The TRP ion channel family.
NAT.REV.NEUROSCI. 2 6387-396 (2001).
4. HARTENECK, C., PLANT, T. AND SCHULTZ, G.
From worm to man: three subfamilies of TRP channels.
TRENDS NEUROSCI. 23 159-166 (2000).
5. HOFMANN, T., OBUKHOV, A.G., SCHAEFER, M., HARTENECK, C., GUDERMANN, T.
AND SCHULTZ, G.
Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol.
NATURE 397 259-263 (1999).
Transient receptor potential (Trp) and related proteins are thought to be
Ca2+ ion channel subunits that mediate capacitative Ca2+ entry in response
to a range of external and internal cell stimuli. Such Ca2+ entry is thought
to be an essential component of cellular responses to many hormones and
growth factors, and acts to replenish intracellular Ca2+ stores that have
been emptied through the action of inositol triphosphate (IP3) and other
agents. In non-excitable cells, i.e. those that lack voltage-gated Ca2+
channels, such as hepatocytes, this mode of Ca2+ entry is thought to be an
important step in generating the oscillations of intracellular Ca2+
concentration that characterise their response to stimulatory agents [1].
Studies on the visual transduction system in Drosophila led to the molecular
cloning of Trp and the cDNA of a related protein, Trp-like, which show
similarity to voltage gated Ca2+ channels in the regions known as S3 through
S6, including the S5-S6 linker that forms the ion-selective channel pore [2].
This provided evidence that Trp and/or related proteins might form mammalian
capacitative Ca2+ entry channels.
A number of Trp and Trp-like channel gene isoforms have now been cloned,
including several mammalian homologues. The Trp family is thought to encode
at least 20 Ca2+-permeable channel proteins. Hydropathy analysis suggests
that they share a common transmembrane (TM) topology. Each family member is
predicted to possess 6 TM domains with intracellular N- and C-termini, which
is similar to the core structure of the pore-forming subunits of the voltage
-gated Na+ and Ca2+ channels. By analogy with these proteins, which have
4 linked domains of 6 TM segments, it is likely that Trp channels are
homo- or heterotetramers of 4 single subunits [3].
The Trp family can be divided on the basis of sequence similarity into 3
subfamilies: short (S), long (L) and osm-like (O) Trp channels [4]. The
STrp subfamily includes Drosophila Trp and Trpl-like, and the mammalian
homologues TrpC1-7. Channels of the STrpC subfamily are activated following
receptor-mediated stimulation of different isoforms of phospholipase C [4].
TrpC3 was originally cloned from human tissue [1]; mouse and rat isoforms
have also been identified. Expression studies have shown that TrpC3 displays
low selectivity for Ca2+ over Na+, and, like TrpC6 and TrpC7, is activated
by diacylglycerol in a protein kinase C-independent manner [5].
TRPCHANNEL3 is a 5-element fingerprint that provides a signature for
transient receptor potential channel 3. The fingerprint was derived from an
initial alignment of 3 sequences: the motifs were drawn from conserved
regions in the cytosolic termini, focusing on those sections that
characterise TrpC3 but distinguish it from other family members - motif 1
lies in the intracellular N-terminal region; and motifs 2 to 5 reside within
the C-terminal cytoplasmic region. A single iteration on SPTR40_18f was
required to reach convergence, no further sequences being identified beyond
the starting set.
SUMMARY INFORMATION
3 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
5| 3 3 3 3 3
4| 0 0 0 0 0
3| 0 0 0 0 0
2| 0 0 0 0 0
--+--------------------------
| 1 2 3 4 5
True positives..
TRP3_MOUSE TRP3_RAT TRP3_HUMAN
PROTEIN TITLES
TRP3_MOUSE Short transient receptor potential channel 3 (TrpC3) (Transi
TRP3_RAT Short transient receptor potential channel 3 (TrpC3) - Rattu
TRP3_HUMAN Short transient receptor potential channel 3 (TrpC3) (Htrp-3
SCAN HISTORY
SPTR40_18f 1 150 NSINGLE
INITIAL MOTIF SETS
TRPCHANNEL31 Length of motif = 13 Motif number = 1
Transient receptor potential channel 3 motif I - 1
PCODE ST INT
SAEPLERHGHKAS TRP3_MOUSE 281 281
SAEPLEVHRHKAS TRP3_HUMAN 293 293
SVEPLERHGHKAS TRP3_RAT 181 181
TRPCHANNEL32 Length of motif = 15 Motif number = 2
Transient receptor potential channel 3 motif II - 1
PCODE ST INT
FVYFIMRITNFSKCR TRP3_MOUSE 704 410
FVYFIMRIVNFPKCR TRP3_HUMAN 716 410
FVYFIMRITNFSKCR TRP3_RAT 604 410
TRPCHANNEL33 Length of motif = 12 Motif number = 3
Transient receptor potential channel 3 motif III - 1
PCODE ST INT
MGNSKSRLNLFT TRP3_MOUSE 730 11
MGNSKSRLNLFT TRP3_HUMAN 742 11
MGNSKSRLNLFT TRP3_RAT 630 11
TRPCHANNEL34 Length of motif = 17 Motif number = 4
Transient receptor potential channel 3 motif IV - 1
PCODE ST INT
NSRVFESHSFNSILNQP TRP3_MOUSE 744 2
NSRVFESHSFNSILNQP TRP3_HUMAN 756 2
NSRVFESHSFNSILNQP TRP3_RAT 644 2
TRPCHANNEL35 Length of motif = 22 Motif number = 5
Transient receptor potential channel 3 motif V - 1
PCODE ST INT
EELAILIHKLSEKLNPSVLRCE TRP3_MOUSE 815 54
EELAILIHKLSEKLNPSMLRCE TRP3_HUMAN 827 54
EELAILIHKLSEKLNPSALRCE TRP3_RAT 715 54
FINAL MOTIF SETS
TRPCHANNEL31 Length of motif = 13 Motif number = 1
Transient receptor potential channel 3 motif I - 1
PCODE ST INT
SAEPLERHGHKAS TRP3_MOUSE 281 281
SVEPLERHGHKAS TRP3_RAT 181 181
SAEPLEVHRHKAS TRP3_HUMAN 293 293
TRPCHANNEL32 Length of motif = 15 Motif number = 2
Transient receptor potential channel 3 motif II - 1
PCODE ST INT
FVYFIMRITNFSKCR TRP3_MOUSE 704 410
FVYFIMRITNFSKCR TRP3_RAT 604 410
FVYFIMRIVNFPKCR TRP3_HUMAN 716 410
TRPCHANNEL33 Length of motif = 12 Motif number = 3
Transient receptor potential channel 3 motif III - 1
PCODE ST INT
MGNSKSRLNLFT TRP3_MOUSE 730 11
MGNSKSRLNLFT TRP3_RAT 630 11
MGNSKSRLNLFT TRP3_HUMAN 742 11
TRPCHANNEL34 Length of motif = 17 Motif number = 4
Transient receptor potential channel 3 motif IV - 1
PCODE ST INT
NSRVFESHSFNSILNQP TRP3_MOUSE 744 2
NSRVFESHSFNSILNQP TRP3_RAT 644 2
NSRVFESHSFNSILNQP TRP3_HUMAN 756 2
TRPCHANNEL35 Length of motif = 22 Motif number = 5
Transient receptor potential channel 3 motif V - 1
PCODE ST INT
EELAILIHKLSEKLNPSVLRCE TRP3_MOUSE 815 54
EELAILIHKLSEKLNPSALRCE TRP3_RAT 715 54
EELAILIHKLSEKLNPSMLRCE TRP3_HUMAN 827 54
User query: Display/Full Code "TRPCHANNEL3"