WORKLIST ENTRIES (1):
TRPCHANNEL4 View alignment Transient receptor potential channel 4 signature
Type of fingerprint: COMPOUND with 6 elements
Links:
PRINTS; PR01097 TRNSRECEPTRP; PR01642 TRPCHANNEL1; PR01643 TRPCHANNEL2
PRINTS; PR01644 TRPCHANNEL3; PR01646 TRPCHANNEL5; PR01647 TRPCHANNEL6
PRINTS; PR01648 TRPCHANNEL7
MIM; 603651
Creation date 13-NOV-2001
1. ZHU, X., JIANG, M., PEYTON, M., BOULAY, G., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Trp, a novel mammalian gene family essential for agonist-activated
capacitative Ca2+ entry.
CELL 85 661-671 (1996).
2. BOULAY, G., ZHU, X., PEYTON, M., JIANG, M., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Cloning and expression of a novel mammalian homolog of Drosophila transient
receptor potential (Trp) involved in calcium entry secondary to activation
of receptors coupled by the Gq class of G protein.
J.BIOL.CHEM. 272 29672-29680 (1997).
3. CLAPHAM, D., RUNNELS, L. AND STRUBING, C.
The TRP ion channel family.
NAT.REV.NEUROSCI. 2 6387-396 (2001).
4. HARTENECK, C., PLANT, T. AND SCHULTZ, G.
From worm to man: three subfamilies of TRP channels.
TRENDS NEUROSCI. 23 159-166 (2000).
5. PHILIPP, S., CAVALIE, A., FREICHEL, M., WISSENBACH, U., ZIMMER, S.,
TROST, C., MARQUART, A., MURAKAMI, M. AND FLOCKERZI, V.
A mammalian capacitative calcium entry channel homologous to Drosophila TRP
and TRPL.
EMBO J. 15 6166-6171 (1996).
6. SCHAEFER, M., PLANT, T., OBUKHOV, A., HOFMANN, T., GUDERMANN, T. AND
SCHULTZ, G.
Receptor-mediated regulation of the nonselective cation channels TRPC4 and
TRPC5.
J.BIOL.CHEM. 275 17517-17526 (2000).
Transient receptor potential (Trp) and related proteins are thought to be
Ca2+ ion channel subunits that mediate capacitative Ca2+ entry in response
to a range of external and internal cell stimuli. Such Ca2+ entry is thought
to be an essential component of cellular responses to many hormones and
growth factors, and acts to replenish intracellular Ca2+ stores that have
been emptied through the action of inositol triphosphate (IP3) and other
agents. In non-excitable cells, i.e. those that lack voltage-gated Ca2+
channels, such as hepatocytes, this mode of Ca2+ entry is thought to be an
important step in generating the oscillations of intracellular Ca2+
concentration that characterise their response to stimulatory agents [1].
Studies on the visual transduction system in Drosophila led to the molecular
cloning of Trp and the cDNA of a related protein, Trp-like, which show
similarity to voltage gated Ca2+ channels in the regions known as S3 through
S6, including the S5-S6 linker that forms the ion-selective channel pore [2].
This provided evidence that Trp and/or related proteins might form mammalian
capacitative Ca2+ entry channels.
A number of Trp and Trp-like channel gene isoforms have now been cloned,
including several mammalian homologues. The Trp family is thought to encode
at least 20 Ca2+-permeable channel proteins. Hydropathy analysis suggests
that they share a common transmembrane (TM) topology. Each family member is
predicted to possess 6 TM domains with intracellular N- and C-termini, which
is similar to the core structure of the pore-forming subunits of the voltage
-gated Na+ and Ca2+ channels. By analogy with these proteins, which have
4 linked domains of 6 TM segments, it is likely that Trp channels are
homo- or heterotetramers of 4 single subunits [3].
The Trp family can be divided on the basis of sequence similarity into 3
subfamilies: short (S), long (L) and osm-like (O) Trp channels [4]. The
STrp subfamily includes Drosophila Trp and Trpl-like, and the mammalian
homologues TrpC1-7. Channels of the STrpC subfamily are activated following
receptor-mediated stimulation of different isoforms of phospholipase C [4].
TrpC4 was originally cloned from bovine tissue [5]; rat, mouse and human
isoforms have also been identified. Expression studies have demonstrated
that TrpC4 is able to form homomeric cation channels that are activated
following stimulation of Gq-coupled receptors and by receptor tyrosine
kinases [6].
TRPCHANNEL4 is a 6-element fingerprint that provides a signature for
transient receptor potential channel 4. The fingerprint was derived from an
initial alignment of 4 sequences: the motifs were drawn from conserved
regions in the C-terminal portion of the alignment, focusing on those
sections that characterise TrpC4 but distinguish it from other family
members - all motifs reside within the the cytoplasmic C-terminus. A single
iteration on SPTR40_18f was required to reach convergence, no further
sequences being identified beyond the starting set.
SUMMARY INFORMATION
4 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
6| 4 4 4 4 4 4
5| 0 0 0 0 0 0
4| 0 0 0 0 0 0
3| 0 0 0 0 0 0
2| 0 0 0 0 0 0
--+-------------------------------
| 1 2 3 4 5 6
True positives..
TRP4_HUMAN TRP4_MOUSE TRP4_RAT TRP4_BOVIN
PROTEIN TITLES
TRP4_HUMAN Short transient receptor potential channel 4 (TrpC4) (trp-re
TRP4_MOUSE Short transient receptor potential channel 4 (TrpC4) (Recept
TRP4_RAT Short transient receptor potential channel 4 (TrpC4) (Trp4)
TRP4_BOVIN Short transient receptor potential channel 4 (TrpC4) (Capaci
SCAN HISTORY
SPTR40_18f 1 150 NSINGLE
INITIAL MOTIF SETS
TRPCHANNEL41 Length of motif = 12 Motif number = 1
Transient receptor potential channel 4 motif I - 1
PCODE ST INT
RGSKLSTIQSAN TRP4_RAT 755 755
RGSKLSTIQSAN TRP4_HUMAN 755 755
RGSKLSTIQSAN TRP4_MOUSE 755 755
RGSKLSTVQSAQ TRP4_BOVIN 757 757
TRPCHANNEL42 Length of motif = 17 Motif number = 2
Transient receptor potential channel 4 motif II - 1
PCODE ST INT
SADSDEKSHSEGNGKDK TRP4_RAT 773 6
SADSDEKSDSEGNSKDK TRP4_HUMAN 774 7
SADSDEKSQSEGNGKDK TRP4_MOUSE 770 3
SADSDEKSDNEGSSKDK TRP4_BOVIN 776 7
TRPCHANNEL43 Length of motif = 23 Motif number = 3
Transient receptor potential channel 4 motif III - 1
PCODE ST INT
KNLSLFDLTTLIHPRSAVIASER TRP4_RAT 791 1
KNFSLFDLTTLIHPRSAAIASER TRP4_HUMAN 792 1
KNLSLFDLTTLIHPRSAAIASER TRP4_MOUSE 788 1
KNFSLFDLTTLIHPRSAAIAAER TRP4_BOVIN 794 1
TRPCHANNEL44 Length of motif = 23 Motif number = 4
Transient receptor potential channel 4 motif IV - 1
PCODE ST INT
LSNGSALVVQEPPREKQRKVNFV TRP4_RAT 816 2
ISNGSALVVQEPPREKQRKVNFV TRP4_HUMAN 817 2
LSNGSALVVQEPPREKQRKVNFV TRP4_MOUSE 813 2
ISNGSALVVQEPPREKQRKVNFV TRP4_BOVIN 819 2
TRPCHANNEL45 Length of motif = 14 Motif number = 5
Transient receptor potential channel 4 motif V - 1
PCODE ST INT
DIKNFGLFHRRSKQ TRP4_RAT 840 1
DIKNFGLFHRRSKQ TRP4_HUMAN 841 1
DIKNFGLFHRRSKQ TRP4_MOUSE 837 1
DIRHFGLFHRRSKQ TRP4_BOVIN 843 1
TRPCHANNEL46 Length of motif = 30 Motif number = 6
Transient receptor potential channel 4 motif VI - 1
PCODE ST INT
SIPGLNEQCVLVDHRERNTDTLGLQVGKRV TRP4_RAT 897 43
SIPGLSEQCVLVDHRERNTDTLGLQVGKRV TRP4_HUMAN 898 43
SIPGLNEQCVLVDHRERNTDTLGLQVGKRV TRP4_MOUSE 894 43
NIPGLSEQCILVDHRERNTDSLGVQVSKRV TRP4_BOVIN 901 44
FINAL MOTIF SETS
TRPCHANNEL41 Length of motif = 12 Motif number = 1
Transient receptor potential channel 4 motif I - 1
PCODE ST INT
RGSKLSTIQSAN TRP4_HUMAN 755 755
RGSKLSTIQSAN TRP4_MOUSE 755 755
RGSKLSTIQSAN TRP4_RAT 755 755
RGSKLSTVQSAQ TRP4_BOVIN 757 757
TRPCHANNEL42 Length of motif = 17 Motif number = 2
Transient receptor potential channel 4 motif II - 1
PCODE ST INT
SADSDEKSDSEGNSKDK TRP4_HUMAN 774 7
SADSDEKSQSEGNGKDK TRP4_MOUSE 770 3
SADSDEKSHSEGNGKDK TRP4_RAT 773 6
SADSDEKSDNEGSSKDK TRP4_BOVIN 776 7
TRPCHANNEL43 Length of motif = 23 Motif number = 3
Transient receptor potential channel 4 motif III - 1
PCODE ST INT
KNFSLFDLTTLIHPRSAAIASER TRP4_HUMAN 792 1
KNLSLFDLTTLIHPRSAAIASER TRP4_MOUSE 788 1
KNLSLFDLTTLIHPRSAVIASER TRP4_RAT 791 1
KNFSLFDLTTLIHPRSAAIAAER TRP4_BOVIN 794 1
TRPCHANNEL44 Length of motif = 23 Motif number = 4
Transient receptor potential channel 4 motif IV - 1
PCODE ST INT
ISNGSALVVQEPPREKQRKVNFV TRP4_HUMAN 817 2
LSNGSALVVQEPPREKQRKVNFV TRP4_MOUSE 813 2
LSNGSALVVQEPPREKQRKVNFV TRP4_RAT 816 2
ISNGSALVVQEPPREKQRKVNFV TRP4_BOVIN 819 2
TRPCHANNEL45 Length of motif = 14 Motif number = 5
Transient receptor potential channel 4 motif V - 1
PCODE ST INT
DIKNFGLFHRRSKQ TRP4_HUMAN 841 1
DIKNFGLFHRRSKQ TRP4_MOUSE 837 1
DIKNFGLFHRRSKQ TRP4_RAT 840 1
DIRHFGLFHRRSKQ TRP4_BOVIN 843 1
TRPCHANNEL46 Length of motif = 30 Motif number = 6
Transient receptor potential channel 4 motif VI - 1
PCODE ST INT
SIPGLSEQCVLVDHRERNTDTLGLQVGKRV TRP4_HUMAN 898 43
SIPGLNEQCVLVDHRERNTDTLGLQVGKRV TRP4_MOUSE 894 43
SIPGLNEQCVLVDHRERNTDTLGLQVGKRV TRP4_RAT 897 43
NIPGLSEQCILVDHRERNTDSLGVQVSKRV TRP4_BOVIN 901 44
User query: Display/Full Code "TRPCHANNEL4"