WORKLIST ENTRIES (1):
TRPCHANNEL6 View alignment Transient receptor potential channel 6 signature
Type of fingerprint: COMPOUND with 5 elements
Links:
PRINTS; PR01097 TRNSRECEPTRP; PR01642 TRPCHANNEL1; PR01643 TRPCHANNEL2
PRINTS; PR01644 TRPCHANNEL3; PR01645 TRPCHANNEL4; PR01646 TRPCHANNEL5
PRINTS; PR01648 TRPCHANNEL7
MIM; 603652
Creation date 13-NOV-2001
1. ZHU, X., JIANG, M., PEYTON, M., BOULAY, G., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Trp, a novel mammalian gene family essential for agonist-activated
capacitative Ca2+ entry.
CELL 85 661-671 (1996).
2. BOULAY, G., ZHU, X., PEYTON, M., JIANG, M., HURST, R., STEFANI, E.
AND BIRNBAUMER, L.
Cloning and expression of a novel mammalian homolog of Drosophila transient
receptor potential (Trp) involved in calcium entry secondary to activation
of receptors coupled by the Gq class of G protein.
J.BIOL.CHEM. 272 29672-29680 (1997).
3. CLAPHAM, D., RUNNELS, L. AND STRUBING, C.
The TRP ion channel family.
NAT.REV.NEUROSCI. 2 6387-396 (2001).
4. HARTENECK, C., PLANT, T. AND SCHULTZ, G.
From worm to man: three subfamilies of TRP channels.
TRENDS NEUROSCI. 23 159-166 (2000).
5. HOFMANN, T., OBUKHOV, A.G., SCHAEFER, M., HARTENECK, C., GUDERMANN, T.
AND SCHULTZ, G.
Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol.
NATURE 397 259-263 (1999).
6. INOUE, R., OKADA, T., ONOUE, H., HARA, Y., SHIMIZU, S., NAITOH, S.,
ITO, Y. AND MORI Y.
The transient receptor potential protein homologue TRP6 is the essential
component of vascular alpha(1)-adrenoceptor-activated Ca(2+)-permeable
cation channel.
CIRC.RES. 88 325-232 (2001).
Transient receptor potential (Trp) and related proteins are thought to be
Ca2+ ion channel subunits that mediate capacitative Ca2+ entry in response
to a range of external and internal cell stimuli. Such Ca2+ entry is thought
to be an essential component of cellular responses to many hormones and
growth factors, and acts to replenish intracellular Ca2+ stores that have
been emptied through the action of inositol triphosphate (IP3) and other
agents. In non-excitable cells, i.e. those that lack voltage-gated Ca2+
channels, such as hepatocytes, this mode of Ca2+ entry is thought to be an
important step in generating the oscillations of intracellular Ca2+
concentration that characterise their response to stimulatory agents [1].
Studies on the visual transduction system in Drosophila led to the molecular
cloning of Trp and the cDNA of a related protein, Trp-like, which show
similarity to voltage gated Ca2+ channels in the regions known as S3 through
S6, including the S5-S6 linker that forms the ion-selective channel pore [2].
This provided evidence that Trp and/or related proteins might form mammalian
capacitative Ca2+ entry channels.
A number of Trp and Trp-like channel gene isoforms have now been cloned,
including several mammalian homologues. The Trp family is thought to encode
at least 20 Ca2+-permeable channel proteins. Hydropathy analysis suggests
that they share a common transmembrane (TM) topology. Each family member is
predicted to possess 6 TM domains with intracellular N- and C-termini, which
is similar to the core structure of the pore-forming subunits of the voltage
-gated Na+ and Ca2+ channels. By analogy with these proteins, which have
4 linked domains of 6 TM segments, it is likely that Trp channels are
homo- or heterotetramers of 4 single subunits [3].
The Trp family can be divided on the basis of sequence similarity into 3
subfamilies: short (S), long (L) and osm-like (O) Trp channels [4]. The
STrp subfamily includes Drosophila Trp and Trpl-like, and the mammalian
homologues TrpC1-7. Channels of the STrpC subfamily are activated following
receptor-mediated stimulation of different isoforms of phospholipase C [4].
TrpC6 was originally cloned from mouse tissue [2]; human, rat and guinea pig
isoforms have also been identified. Expression studies have demonstrated
that TrpC6 displays low selectivity for Ca2+ over Na+, and, like TrpC3 and
TrpC7, is activated by diacylglycerol in a protein kinase C-independent
manner [5]. TrpC6 has recently been shown to be an essential component of
the alpha1-adrenoceptor-activated cation channel [6].
TRPCHANNEL6 is a 5-element fingerprint that provides a signature for
transient receptor potential channel 6. The fingerprint was derived from an
initial alignment of 6 sequences: the motifs were drawn from conserved
regions in the loop and C-terminal portions of the alignment, focusing on
those sections that characterise TrpC6 but distinguish it from other family
members - motifs 1 and 2 lie in the second extracellular loop; and motifs
3-5 reside within the cytoplasmic C-terminal region. A single iteration on
SPTR40_18f was required to reach convergence, no further sequences being
identified beyond the starting set. A single partial match was found,
Q9ESX8, a splice variant of the guinea pig TrpC6, which lacks a portion
of the C-terminal section.
SUMMARY INFORMATION
6 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
5| 6 6 6 6 6
4| 0 0 0 0 0
3| 0 0 0 0 0
2| 1 1 0 0 0
--+--------------------------
| 1 2 3 4 5
True positives..
Q99N76 Q99N78 Q99N77 TRP6_MOUSE
TRP6_HUMAN Q9ESX6
Subfamily: Codes involving 2 elements
Subfamily True positives..
Q9ESX8
PROTEIN TITLES
Q99N76 TRANSIENT RECEPTOR POTENTIAL CA2+ CHANNEL 6C - Rattus norveg
Q99N78 TRANSIENT RECEPTOR POTENTIAL CA2+ CHANNEL 6A - Rattus norveg
Q99N77 TRANSIENT RECEPTOR POTENTIAL CA2+ CHANNEL 6B - Rattus norveg
TRP6_MOUSE Short transient receptor potential channel 6 (TrpC6) (Calciu
TRP6_HUMAN Short transient receptor potential channel 6 (TrpC6) - Homo
Q9ESX6 PUTATIVE CAPACITATIVE CALCIUM ENTRY CHANNEL - Cavia porcellu
Q9ESX8 TRPC6 CATION CHANNEL, PUTATIVE ALTERNATIVE SPLICE VARIANT GP
SCAN HISTORY
SPTR40_18f 1 200 NSINGLE
INITIAL MOTIF SETS
TRPCHANNEL61 Length of motif = 22 Motif number = 1
Transient receptor potential channel 6 motif I - 1
PCODE ST INT
WHASKAQSIIDANDTLKDLTKV Q99N78 548 548
WHASKAQSIIDANDTLKDLTKV Q9ESX6 511 511
WHASKAQSIIDANDTLKDLTKV TRP6_MOUSE 548 548
WHASKAQSIIDANDTLKDLTKV TRP6_HUMAN 549 549
WHASKAQSIIDANDTLKDLTKV Q99N76 494 494
WHASKAQSIIDANDTLKDLTKV Q99N77 494 494
TRPCHANNEL62 Length of motif = 13 Motif number = 2
Transient receptor potential channel 6 motif II - 1
PCODE ST INT
GDNVKYYNLARIK Q99N78 572 2
GEDVKYYNLARIK Q9ESX6 535 2
GDNVKYYNLARIK TRP6_MOUSE 572 2
GDNVKYYNLARIK TRP6_HUMAN 573 2
GDNVKYYNLARIK Q99N76 518 2
GDNVKYYNLARIK Q99N77 518 2
TRPCHANNEL63 Length of motif = 30 Motif number = 3
Transient receptor potential channel 6 motif III - 1
PCODE ST INT
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N78 803 218
RNEEKKFGILGSHEDLSKLSVDKKQLGQNK Q9ESX6 766 218
RNEEKKFGISGSHEDLSKFSLDKNQLAHNK TRP6_MOUSE 803 218
INEEKKLGILGSHEDLSKLSLDKKQVGHNK TRP6_HUMAN 804 218
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N76 681 150
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N77 749 218
TRPCHANNEL64 Length of motif = 20 Motif number = 4
Transient receptor potential channel 6 motif IV - 1
PCODE ST INT
STRSSEDFHLNSFSNPPRQY Q99N78 835 2
SIRSSEDFHLNSFNNPPRQY Q9ESX6 798 2
STRSSEDYHLNSFSNPPRQY TRP6_MOUSE 835 2
SIRSSEDFHLNSFNNPPRQY TRP6_HUMAN 836 2
STRSSEDFHLNSFSNPPRQY Q99N76 713 2
STRSSEDFHLNSFSNPPRQY Q99N77 781 2
TRPCHANNEL65 Length of motif = 15 Motif number = 5
Transient receptor potential channel 6 motif V - 1
PCODE ST INT
GERLSLESKQEESRR Q99N78 916 61
GEKLSSEPKQEEINR Q9ESX6 879 61
GERLSLEPKLEESRR TRP6_MOUSE 916 61
GEKLSMEPNQEETNR TRP6_HUMAN 917 61
GERLSLESKQEESRR Q99N76 794 61
GERLSLESKQEESRR Q99N77 862 61
FINAL MOTIF SETS
TRPCHANNEL61 Length of motif = 22 Motif number = 1
Transient receptor potential channel 6 motif I - 1
PCODE ST INT
WHASKAQSIIDANDTLKDLTKV Q99N76 494 494
WHASKAQSIIDANDTLKDLTKV Q99N78 548 548
WHASKAQSIIDANDTLKDLTKV Q99N77 494 494
WHASKAQSIIDANDTLKDLTKV TRP6_MOUSE 548 548
WHASKAQSIIDANDTLKDLTKV TRP6_HUMAN 549 549
WHASKAQSIIDANDTLKDLTKV Q9ESX6 511 511
TRPCHANNEL62 Length of motif = 13 Motif number = 2
Transient receptor potential channel 6 motif II - 1
PCODE ST INT
GDNVKYYNLARIK Q99N76 518 2
GDNVKYYNLARIK Q99N78 572 2
GDNVKYYNLARIK Q99N77 518 2
GDNVKYYNLARIK TRP6_MOUSE 572 2
GDNVKYYNLARIK TRP6_HUMAN 573 2
GEDVKYYNLARIK Q9ESX6 535 2
TRPCHANNEL63 Length of motif = 30 Motif number = 3
Transient receptor potential channel 6 motif III - 1
PCODE ST INT
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N76 681 150
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N78 803 218
RNEEKKFGILGSHEDLSKFSLDRNQLAHNK Q99N77 749 218
RNEEKKFGISGSHEDLSKFSLDKNQLAHNK TRP6_MOUSE 803 218
INEEKKLGILGSHEDLSKLSLDKKQVGHNK TRP6_HUMAN 804 218
RNEEKKFGILGSHEDLSKLSVDKKQLGQNK Q9ESX6 766 218
TRPCHANNEL64 Length of motif = 20 Motif number = 4
Transient receptor potential channel 6 motif IV - 1
PCODE ST INT
STRSSEDFHLNSFSNPPRQY Q99N76 713 2
STRSSEDFHLNSFSNPPRQY Q99N78 835 2
STRSSEDFHLNSFSNPPRQY Q99N77 781 2
STRSSEDYHLNSFSNPPRQY TRP6_MOUSE 835 2
SIRSSEDFHLNSFNNPPRQY TRP6_HUMAN 836 2
SIRSSEDFHLNSFNNPPRQY Q9ESX6 798 2
TRPCHANNEL65 Length of motif = 15 Motif number = 5
Transient receptor potential channel 6 motif V - 1
PCODE ST INT
GERLSLESKQEESRR Q99N76 794 61
GERLSLESKQEESRR Q99N78 916 61
GERLSLESKQEESRR Q99N77 862 61
GERLSLEPKLEESRR TRP6_MOUSE 916 61
GEKLSMEPNQEETNR TRP6_HUMAN 917 61
GEKLSSEPKQEEINR Q9ESX6 879 61
User query: Display/Full Code "TRPCHANNEL6"