WORKLIST ENTRIES (1):
VASOACTVEIPR View alignment Vasoactive intestinal peptide receptor signature
Type of fingerprint: COMPOUND with 4 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR01154 VIP1RECEPTOR; PR01155 VIP2RECEPTOR; PR01156 PACAPRECEPTR
INTERPRO; IPR001571
Creation date 03-MAR-1996; UPDATE 07-JUN-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.
Molecular cloning and expression of a cDNA encoding the secretin receptor.
EMBO J. 10 1635-1641 (1991).
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H.,
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor.
SCIENCE 254 1022-1024 (1991).
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F.,
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
6. WATSON, S. AND ARKINSTALL, S.
Vasoactive intestinal polypeptide family.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.278-283.
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
families: the secretin-like receptors thus bear their own unique '7TM'
signature.
Vasoactive intestinal polypeptide (VIP) has a wide physiological profile.
In the periphery, it induces relaxation in smooth muscle; inhibits
secretion in certain tissues, but stimulates secretion in others; and
modulates activity of cells in the immune system [6]. In the CNS, it has a
range of both excitatory and inhibitory actions. VIP receptors are
distributed widely in the periphery, and occur throughout the gastro-
intestinal tract and genitourinary system, other smooth muscles and
secretory glands. In the CNS, they are found abundantly in, e.g. the cortex,
hippocampus and thalamus [6]. All VIP receptors activate adenylyl cyclase.
VASOACTVEIPR is a 4-element fingerprint that provides a signature for the
vasoactive intestinal peptide receptors. The fingerprint was derived from
an initial alignment of 5 sequences: the motifs were drawn from conserved
sections within the N- and C-termini, focusing on those areas of the
alignment that characterise the VIP receptors but distinguish them from the
rest of the secretin-like family - motifs 1-3 lie in the N-terminal region
preceding the first TM domain, and motif 4 lies at the C-terminus. Two
iterations on OWL27.0 were required to reach convergence, at which point a
true set comprising 6 sequences was identified. Seven partial matches were
also found, all of which are members of the secretin-like family.
An update on SPTR37_9f identified a true set of 6 sequences.
SUMMARY INFORMATION
6 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
4| 6 6 6 6
3| 0 0 0 0
2| 0 0 0 0
--+---------------------
| 1 2 3 4
True positives..
VIPS_MOUSE VIPS_RAT VIPS_HUMAN VIPR_HUMAN
VIPR_PIG VIPR_RAT
PROTEIN TITLES
VIPS_MOUSE VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-
VIPS_RAT VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-
VIPS_HUMAN VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-
VIPR_HUMAN VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-
VIPR_PIG VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-
VIPR_RAT VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-
SCAN HISTORY
OWL27_0 2 100 NSINGLE
SPTR37_9f 2 7 NSINGLE
INITIAL MOTIF SETS
VASOACTVEIPR1 Length of motif = 12 Motif number = 1
VIP receptor motif I - 1
PCODE ST INT
PKVFSNFYSRPG VIPS_MOUSE 75 75
PKVFSNFYSRPG VIPS_RAT 75 75
PLIFKLFSSIQG VIPR_HUMAN 87 87
PKVFSNFYSKAG VIPS_HUMAN 76 76
PLIFQLFAPIHG VIPR_RAT 87 87
VASOACTVEIPR2 Length of motif = 16 Motif number = 2
VIP receptor motif II - 1
PCODE ST INT
NISKNCTSDGWSETFP VIPS_MOUSE 87 0
NISKNCTSDGWSETFP VIPS_RAT 87 0
NVSRSCTDEGWTHLEP VIPR_HUMAN 100 1
NISKNCTSDGWSETFP VIPS_HUMAN 88 0
NISRSCTEEGWSQLEP VIPR_RAT 100 1
VASOACTVEIPR3 Length of motif = 11 Motif number = 3
VIP receptor motif III - 1
PCODE ST INT
DFIDACGYNDP VIPS_MOUSE 103 0
DFIDACGYNDP VIPS_RAT 103 0
PYPIACGLDDK VIPR_HUMAN 117 1
DFVDACGYSDP VIPS_HUMAN 104 0
PYHIACGLNDR VIPR_RAT 117 1
VASOACTVEIPR4 Length of motif = 12 Motif number = 4
VIP receptor motif IV - 1
PCODE ST INT
GSRTQSFLQSET VIPS_MOUSE 423 309
GSRTQSFLQSET VIPS_RAT 423 309
ARRSSSFQAEVS VIPR_HUMAN 444 316
ASRAQSFLQTET VIPS_HUMAN 424 309
ARRSSSFQAEVS VIPR_RAT 446 318
FINAL MOTIF SETS
VASOACTVEIPR1 Length of motif = 12 Motif number = 1
VIP receptor motif I - 2
PCODE ST INT
PKVFSNFYSRPG VIPS_MOUSE 75 75
PKVFSNFYSRPG VIPS_RAT 75 75
PKVFSNFYSKAG VIPS_HUMAN 76 76
PLIFKLFSSIQG VIPR_HUMAN 87 87
PLIFKLFSPTQG VIPR_PIG 88 88
PLIFQLFAPIHG VIPR_RAT 87 87
VASOACTVEIPR2 Length of motif = 16 Motif number = 2
VIP receptor motif II - 2
PCODE ST INT
NISKNCTSDGWSETFP VIPS_MOUSE 87 0
NISKNCTSDGWSETFP VIPS_RAT 87 0
NISKNCTSDGWSETFP VIPS_HUMAN 88 0
NVSRSCTDEGWTHLEP VIPR_HUMAN 100 1
NVSRNCTDEGWTPLEP VIPR_PIG 101 1
NISRSCTEEGWSQLEP VIPR_RAT 100 1
VASOACTVEIPR3 Length of motif = 11 Motif number = 3
VIP receptor motif III - 2
PCODE ST INT
DFIDACGYNDP VIPS_MOUSE 103 0
DFIDACGYNDP VIPS_RAT 103 0
DFVDACGYSDP VIPS_HUMAN 104 0
PYPIACGLDDK VIPR_HUMAN 117 1
PYPIACGMDDK VIPR_PIG 118 1
PYHIACGLNDR VIPR_RAT 117 1
VASOACTVEIPR4 Length of motif = 12 Motif number = 4
VIP receptor motif IV - 2
PCODE ST INT
GSRTQSFLQSET VIPS_MOUSE 423 309
GSRTQSFLQSET VIPS_RAT 423 309
GSRAQSFLQTET VIPS_HUMAN 424 309
ARRSSSFQAEVS VIPR_HUMAN 444 316
ARRSSSFQAEVS VIPR_PIG 445 316
ARRSSSFQAEVS VIPR_RAT 446 318
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