WORKLIST ENTRIES (1):
VIP1RECEPTOR View alignment Vasoactive intestinal peptide receptor 1 signature
Type of fingerprint: COMPOUND with 8 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00491 VASOACTVEIPR; PR01155 VIP2RECEPTOR; PR01156 PACAPRECEPTR
INTERPRO; IPR001771
Creation date 23-MAY-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.
Molecular cloning and expression of a cDNA encoding the secretin receptor.
EMBO J. 10 1635-1641 (1991).
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H.,
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor.
SCIENCE 254 1022-1024 (1991).
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F.,
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
6. WATSON, S. AND ARKINSTALL, S.
Vasoactive intestinal polypeptide family.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.278-283.
7. WEI, Y. AND MOJSOV, S.
Tissue specific expression of different human receptor types for pituitary
adenylate cyclase activating polypeptide and vasoactive intestinal
polypeptide: implications for their role in human physiology.
J.NEUROENDOCRINOL. 8 811-817 (1996).
8. DELGADO, M., MARTINEZ, C., JOHNSON, M.C., GOMARIZ, R.P. AND GANEA, D.
Differential expression of vasoactive intestinal peptide receptors 1 and 2
(VIP-R1 and VIP-R2) mRNA in murine lymphocytes.
J.NEUROIMMUNOL. 68 27-38 (1996).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
families: the secretin-like receptors thus bear their own unique '7TM'
signature.
Vasoactive intestinal polypeptide (VIP) has a wide physiological profile.
In the periphery, it induces relaxation in smooth muscle; inhibits
secretion in certain tissues, but stimulates secretion in others; and
modulates activity of cells in the immune system [6]. In the CNS, it has a
range of both excitatory and inhibitory actions. VIP receptors are
distributed widely in the periphery, and occur throughout the gastro-
intestinal tract and genitourinary system, other smooth muscles and
secretory glands. In the CNS, they are found abundantly in, e.g. the cortex,
hippocampus and thalamus [6]. All VIP receptors activate adenylyl cyclase.
There are two structurally distinct receptors that recognise VIP peptides
and pituitary adenylate cyclase activating polypeptide (PACAP) with similar
affinities (PACAP/VIPR-1, PACAP/VIPR-2), as well as a specific receptor for
the PACAP peptide (PACAP-1). RNA transcripts for all three receptor types
are found in human heart, brain and adipose tissue [7]. VIPR-1 is
constitutively expressed, while the expression of VIPR-2 is induced only
following stimulation through the TCR-associated CD3 complex [8]. VIP
induces the expression of the VIPR-2 gene in the absence of additional
stimuli. Differential expression and regulation of the two VIP receptors
in T lymphocytes suggests different physiological roles in mediating the
immunomodulatory activities of VIP and related neuropeptides [8].
VIP1RECEPTOR is an 8-element fingerprint that provides a signature for
vasoactive intestinal peptide type 1 receptors. The fingerprint was derived
from an initial alignment of 3 sequences: the motifs were drawn from
conserved regions spanning the full alignment length, focusing on those
areas that characterise the type 1 VIP receptors but distinguish them from
the rest of the VIP receptor family - motifs 1-3 lie in the N-terminal
region immediately preceding TM domain 1; motif 4 spans the loop between
TM domains 2 and 3; motif 5 resides in the loop between TM domains 3 and 4;
motif 6 spans the loop between TM domains 6 and 7; and motifs 7 and 8 reside
at the C-terminus. A single iteration on SPTR37_9f was required to reach
convergence, no further sequences being identified beyond the starting set.
A single partial match was found, VIPR_CARAU, a goldfish type 1 VIP receptor
that matches motifs 4, 5, 7 and 8.
SUMMARY INFORMATION
3 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
8| 3 3 3 3 3 3 3 3
7| 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0
4| 0 0 0 1 1 0 1 1
3| 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0
--+-----------------------------------------
| 1 2 3 4 5 6 7 8
True positives..
VIPR_PIG VIPR_HUMAN VIPR_RAT
Subfamily: Codes involving 4 elements
Subfamily True positives..
VIPR_CARAU
PROTEIN TITLES
VIPR_PIG VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-
VIPR_HUMAN VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-
VIPR_RAT VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 1 PRECURSOR (VIP-
VIPR_CARAU VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR (VIP-R) (VIP RECE
SCAN HISTORY
SPTR37_9f 1 50 NSINGLE
INITIAL MOTIF SETS
VIP1RECEPTOR1 Length of motif = 22 Motif number = 1
Vasoactive intestinal polypeptide receptor 1 motif I - 1
PCODE ST INT
ECEYLQLIEIQRQQCLEEAQLE VIPR_RAT 36 36
ECDYVQMIEVQHKQCLEEAQLE VIPR_HUMAN 36 36
ECDYLQMIKVQHKQCLEEAQLE VIPR_PIG 37 37
VIP1RECEPTOR2 Length of motif = 17 Motif number = 2
Vasoactive intestinal polypeptide receptor 1 motif II - 1
PCODE ST INT
WPTTPRGQAVVLDCPLI VIPR_RAT 73 15
WPATPRGQVVVLACPLI VIPR_HUMAN 73 15
WPATPRGQVVVLACPLI VIPR_PIG 74 15
VIP1RECEPTOR3 Length of motif = 21 Motif number = 3
Vasoactive intestinal polypeptide receptor 1 motif III - 1
PCODE ST INT
LEPGPYHIACGLNDRASSLDE VIPR_RAT 113 23
LEPGPYPIACGLDDKAASLDE VIPR_HUMAN 113 23
LEPGPYPIACGMDDKASGLDE VIPR_PIG 114 23
VIP1RECEPTOR4 Length of motif = 15 Motif number = 4
Vasoactive intestinal polypeptide receptor 1 motif IV - 1
PCODE ST INT
FNSGEIDHCSEASVG VIPR_RAT 201 67
FDSGESDQCSEGSVG VIPR_HUMAN 200 66
FDSEESDHCSKGSVG VIPR_PIG 201 66
VIP1RECEPTOR5 Length of motif = 14 Motif number = 5
Vasoactive intestinal polypeptide receptor 1 motif V - 1
PCODE ST INT
VSFFSERKYFWGYI VIPR_RAT 247 31
VSFFSERKYFWGYI VIPR_HUMAN 246 31
VSFFSERKYFWGYI VIPR_PIG 247 31
VIP1RECEPTOR6 Length of motif = 13 Motif number = 6
Vasoactive intestinal polypeptide receptor 1 motif VI - 1
PCODE ST INT
FFPDNFKAQVKMV VIPR_RAT 361 100
FFPDNFKPEVKMV VIPR_HUMAN 359 99
FFPDNFKAEVKMV VIPR_PIG 360 99
VIP1RECEPTOR7 Length of motif = 23 Motif number = 7
Vasoactive intestinal polypeptide receptor 1 motif VII - 1
PCODE ST INT
RWHLQGVLGWSSKSQHPWGGSNG VIPR_RAT 407 33
RWHLQGVLGWNPKYRHPSGGSNG VIPR_HUMAN 405 33
RWHQQGVLGWDSKYQHPSGGSNG VIPR_PIG 406 33
VIP1RECEPTOR8 Length of motif = 24 Motif number = 8
Vasoactive intestinal polypeptide receptor 1 motif VIII - 1
PCODE ST INT
CSTQVSMLTRVSPSARRSSSFQAE VIPR_RAT 432 2
CSTQVSMLTRVSPGARRSSSFQAE VIPR_HUMAN 430 2
CSTQVSMLTRVSPSARRSSSFQAE VIPR_PIG 431 2
FINAL MOTIF SETS
VIP1RECEPTOR1 Length of motif = 22 Motif number = 1
Vasoactive intestinal polypeptide receptor 1 motif I - 1
PCODE ST INT
ECDYLQMIKVQHKQCLEEAQLE VIPR_PIG 37 37
ECDYVQMIEVQHKQCLEEAQLE VIPR_HUMAN 36 36
ECEYLQLIEIQRQQCLEEAQLE VIPR_RAT 36 36
VIP1RECEPTOR2 Length of motif = 17 Motif number = 2
Vasoactive intestinal polypeptide receptor 1 motif II - 1
PCODE ST INT
WPATPRGQVVVLACPLI VIPR_PIG 74 15
WPATPRGQVVVLACPLI VIPR_HUMAN 73 15
WPTTPRGQAVVLDCPLI VIPR_RAT 73 15
VIP1RECEPTOR3 Length of motif = 21 Motif number = 3
Vasoactive intestinal polypeptide receptor 1 motif III - 1
PCODE ST INT
LEPGPYPIACGMDDKASGLDE VIPR_PIG 114 23
LEPGPYPIACGLDDKAASLDE VIPR_HUMAN 113 23
LEPGPYHIACGLNDRASSLDE VIPR_RAT 113 23
VIP1RECEPTOR4 Length of motif = 15 Motif number = 4
Vasoactive intestinal polypeptide receptor 1 motif IV - 1
PCODE ST INT
FDSEESDHCSKGSVG VIPR_PIG 201 66
FDSGESDQCSEGSVG VIPR_HUMAN 200 66
FNSGEIDHCSEASVG VIPR_RAT 201 67
VIP1RECEPTOR5 Length of motif = 14 Motif number = 5
Vasoactive intestinal polypeptide receptor 1 motif V - 1
PCODE ST INT
VSFFSERKYFWGYI VIPR_PIG 247 31
VSFFSERKYFWGYI VIPR_HUMAN 246 31
VSFFSERKYFWGYI VIPR_RAT 247 31
VIP1RECEPTOR6 Length of motif = 13 Motif number = 6
Vasoactive intestinal polypeptide receptor 1 motif VI - 1
PCODE ST INT
FFPDNFKAEVKMV VIPR_PIG 360 99
FFPDNFKPEVKMV VIPR_HUMAN 359 99
FFPDNFKAQVKMV VIPR_RAT 361 100
VIP1RECEPTOR7 Length of motif = 23 Motif number = 7
Vasoactive intestinal polypeptide receptor 1 motif VII - 1
PCODE ST INT
RWHQQGVLGWDSKYQHPSGGSNG VIPR_PIG 406 33
RWHLQGVLGWNPKYRHPSGGSNG VIPR_HUMAN 405 33
RWHLQGVLGWSSKSQHPWGGSNG VIPR_RAT 407 33
VIP1RECEPTOR8 Length of motif = 24 Motif number = 8
Vasoactive intestinal polypeptide receptor 1 motif VIII - 1
PCODE ST INT
CSTQVSMLTRVSPSARRSSSFQAE VIPR_PIG 431 2
CSTQVSMLTRVSPGARRSSSFQAE VIPR_HUMAN 430 2
CSTQVSMLTRVSPSARRSSSFQAE VIPR_RAT 432 2
User query: Display/Full Code "VIP1RECEPTOR"