WORKLIST ENTRIES (1):
VIP2RECEPTOR View alignment Vasoactive intestinal peptide receptor 2 signature
Type of fingerprint: COMPOUND with 10 elements
Links:
PRINTS; PR00237 GPCRRHODOPSN; PR00247 GPCRCAMP; PR00248 GPCRMGR
PRINTS; PR00249 GPCRSECRETIN; PR00250 GPCRSTE2; PR00899 GPCRSTE3
PRINTS; PR00251 BACTRLOPSIN
PRINTS; PR00491 VASOACTVEIPR; PR01154 VIP1RECEPTOR; PR01156 PACAPRECEPTR
INTERPRO; IPR002284
Creation date 23-MAY-1999
1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ISHIHARA T., NAKAMURA S., KAZIRO, Y., TAKAHASHI, T., TAKAHASHI, K.
AND NAGATA, S.
Molecular cloning and expression of a cDNA encoding the secretin receptor.
EMBO J. 10 1635-1641 (1991).
3. LIN, H.Y., HARRIS, T.L., FLANNERY, M.S., ARUFFO, A., KAJI, E.H.,
GORN, A., KOLAKOWSKI, L.F., LODISH, H.F. AND GOLDRING, S.R.
Expression cloning of adenylate cyclase-coupled calcitonin receptor.
SCIENCE 254 1022-1024 (1991).
4. JUEPPNER, H., ABOU-SAMRA, A.-B., FREEMAN, M., KONG, X.F.,
SCHIPANI, E., RICHARDS, J., KOLALOWSKI, L.F., HOCK, J., POTTS, J.T.,
KRONENBERG, H.M. AND SEGRE, G.E.
A G protein linked receptor for parathyroid hormone and parathyroid
hormone-related peptide.
SCIENCE 254 1024-1026 (1991).
5. ISHIHARA, T., SHIGEMOTO, R., MORI, K., TAKAHASHI, K. AND NAGATA, S.
Functional expression and tissue distribution of a novel receptor for
vasoactive intestinal polypeptide.
NEURON 8(4) 811-819 (1992).
6. WATSON, S. AND ARKINSTALL, S.
Vasoactive intestinal polypeptide family.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.278-283.
7. WEI, Y. AND MOJSOV, S.
Tissue specific expression of different human receptor types for pituitary
adenylate cyclase activating polypeptide and vasoactive intestinal
polypeptide: implications for their role in human physiology.
J.NEUROENDOCRINOL. 8 811-817 (1996).
8. DELGADO, M., MARTINEZ, C., JOHNSON, M.C., GOMARIZ, R.P. AND GANEA, D.
Differential expression of vasoactive intestinal peptide receptors 1 and 2
(VIP-R1 and VIP-R2) mRNA in murine lymphocytes.
J.NEUROIMMUNOL. 68 27-38 (1996).
G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The secretin-like GPCRs include secretin [2], calcitonin [3], parathyroid
hormone/parathyroid hormone-related peptides [4] and vasoactive intestinal
peptide [5], all of which activate adenylyl cyclase and the phosphatidyl-
inositol-calcium pathway. The amino acid sequences of the receptors contain
high proportions of hydrophobic residues grouped into 7 domains, in a manner
reminiscent of the rhodopsins and other receptors believed to interact with
G proteins. However, while a similar 3D framework has been proposed to
account for this, there is no significant sequence identity between these
families: the secretin-like receptors thus bear their own unique '7TM'
signature.
Vasoactive intestinal polypeptide (VIP) has a wide physiological profile.
In the periphery, it induces relaxation in smooth muscle; inhibits
secretion in certain tissues, but stimulates secretion in others; and
modulates activity of cells in the immune system [6]. In the CNS, it has a
range of both excitatory and inhibitory actions. VIP receptors are
distributed widely in the periphery, and occur throughout the gastro-
intestinal tract and genitourinary system, other smooth muscles and
secretory glands. In the CNS, they are found abundantly in, e.g. the cortex,
hippocampus and thalamus [6]. All VIP receptors activate adenylyl cyclase.
There are two structurally distinct receptors that recognise VIP peptides
and pituitary adenylate cyclase activating polypeptide (PACAP) with similar
affinities (PACAP/VIPR-1, PACAP/VIPR-2), as well as a specific receptor for
the PACAP peptide (PACAP-1). RNA transcripts for all three receptor types
are found in human heart, brain and adipose tissue [7]. By contrast with
VIPR-1, which is constitutively expressed, the expression of VIPR-2 is
induced only following stimulation through the TCR-associated CD3 complex [8].
VIP induces the expression of the VIPR-2 gene in the absence of additional
stimuli. Differential expression and regulation of the two VIP receptors in
T lymphocytes suggests different physiological roles in mediating the
immunomodulatory activities of VIP and related neuropeptides [8].
VIP2RECEPTOR is a 10-element fingerprint that provides a signature for
vasoactive intestinal peptide type 2 receptors. The fingerprint was derived
from an initial alignment of 3 sequences: the motifs were drawn from
conserved regions spanning the full alignment length, focusing on those
areas that characterise the type 2 VIP receptors but distinguish them from
the rest of the VIP receptor family - motifs 1-5 lie in the N-terminal
region immediately preceding TM domain 1; motif 6 spans the loop between
TM domains 2 and 3; motif 7 lies in the loop between TM domains 3 and 4;
motif 8 spans the loop between TM domains 6 and 7; and motifs 9 and 10
reside at the C-terminus. A single iteration on SPTR37_9f was required to
reach convergence, no further sequences being identified beyond the
starting set.
SUMMARY INFORMATION
3 codes involving 10 elements
0 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
10| 3 3 3 3 3 3 3 3 3 3
9| 0 0 0 0 0 0 0 0 0 0
8| 0 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0 0 0
--+---------------------------------------------------
| 1 2 3 4 5 6 7 8 9 10
True positives..
VIPS_RAT VIPS_MOUSE VIPS_HUMAN
PROTEIN TITLES
VIPS_RAT VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-
VIPS_MOUSE VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-
VIPS_HUMAN VASOACTIVE INTESTINAL POLYPEPTIDE RECEPTOR 2 PRECURSOR (VIP-
SCAN HISTORY
SPTR37_9f 1 50 NSINGLE
INITIAL MOTIF SETS
VIP2RECEPTOR1 Length of motif = 19 Motif number = 1
Vasoactive intestinal polypeptide receptor 2 motif I - 1
PCODE ST INT
CWLLVRVSSIHPECRFHLE VIPS_MOUSE 11 11
CWLLAPVNSIHPECRFHLE VIPS_HUMAN 12 12
CWLLVRVSSIHPECRFHLE VIPS_RAT 11 11
VIP2RECEPTOR2 Length of motif = 20 Motif number = 2
Vasoactive intestinal polypeptide receptor 2 motif II - 1
PCODE ST INT
QEEETKCAELLSSQTENQRA VIPS_MOUSE 31 1
QEEETKCAELLRSQTEKHKA VIPS_HUMAN 32 1
QEEETKCAELLSSQMENHRA VIPS_RAT 31 1
VIP2RECEPTOR3 Length of motif = 13 Motif number = 3
Vasoactive intestinal polypeptide receptor 2 motif III - 1
PCODE ST INT
WRPADVGETVTVP VIPS_MOUSE 61 10
WRPANVGETVTVP VIPS_HUMAN 62 10
WRPADIGETVTVP VIPS_RAT 61 10
VIP2RECEPTOR4 Length of motif = 14 Motif number = 4
Vasoactive intestinal polypeptide receptor 2 motif IV - 1
PCODE ST INT
KVFSNFYSRPGNIS VIPS_MOUSE 76 2
KVFSNFYSKAGNIS VIPS_HUMAN 77 2
KVFSNFYSRPGNIS VIPS_RAT 76 2
VIP2RECEPTOR5 Length of motif = 15 Motif number = 5
Vasoactive intestinal polypeptide receptor 2 motif V - 1
PCODE ST INT
EDESKISFYILVKAI VIPS_MOUSE 114 24
EDESKITFYILVKAI VIPS_HUMAN 115 24
EDESKITFYILVKAI VIPS_RAT 114 24
VIP2RECEPTOR6 Length of motif = 18 Motif number = 6
Vasoactive intestinal polypeptide receptor 2 motif VI - 1
PCODE ST INT
YSSSGLLRCHDQPASWVG VIPS_MOUSE 183 54
YSSSGTLHCPDQPSSWVG VIPS_HUMAN 184 54
YSSSGTLRCHDQPGSWVG VIPS_RAT 183 54
VIP2RECEPTOR7 Length of motif = 16 Motif number = 7
Vasoactive intestinal polypeptide receptor 2 motif VII - 1
PCODE ST INT
VAILPPSRCFLAYLLI VIPS_MOUSE 231 30
VAMLPPRRCFLAYLLI VIPS_HUMAN 232 30
VAILPPSRCFLAYLLI VIPS_RAT 231 30
VIP2RECEPTOR8 Length of motif = 12 Motif number = 8
Vasoactive intestinal polypeptide receptor 2 motif VIII - 1
PCODE ST INT
PIGISSTYQILF VIPS_MOUSE 347 100
PISISSKYQILF VIPS_HUMAN 348 100
PIGISSTYQILF VIPS_RAT 347 100
VIP2RECEPTOR9 Length of motif = 16 Motif number = 9
Vasoactive intestinal polypeptide receptor 2 motif IX - 1
PCODE ST INT
WRGLCLTQAGSRDYRL VIPS_MOUSE 389 30
WRSRCPTPSASRDYRV VIPS_HUMAN 390 30
WRGLCLTQPGSRDYRL VIPS_RAT 389 30
VIP2RECEPTOR10 Length of motif = 24 Motif number = 10
Vasoactive intestinal polypeptide receptor 2 motif X - 1
PCODE ST INT
SRNGSESALQIHRGSRTQSFLQSE VIPS_MOUSE 410 5
SRNGSEGALQFHRGSRAQSFLQTE VIPS_HUMAN 411 5
SRNGSESALQIHRGSRTQSFLQSE VIPS_RAT 410 5
FINAL MOTIF SETS
VIP2RECEPTOR1 Length of motif = 19 Motif number = 1
Vasoactive intestinal polypeptide receptor 2 motif I - 1
PCODE ST INT
CWLLVRVSSIHPECRFHLE VIPS_RAT 11 11
CWLLVRVSSIHPECRFHLE VIPS_MOUSE 11 11
CWLLAPVNSIHPECRFHLE VIPS_HUMAN 12 12
VIP2RECEPTOR2 Length of motif = 20 Motif number = 2
Vasoactive intestinal polypeptide receptor 2 motif II - 1
PCODE ST INT
QEEETKCAELLSSQMENHRA VIPS_RAT 31 1
QEEETKCAELLSSQTENQRA VIPS_MOUSE 31 1
QEEETKCAELLRSQTEKHKA VIPS_HUMAN 32 1
VIP2RECEPTOR3 Length of motif = 13 Motif number = 3
Vasoactive intestinal polypeptide receptor 2 motif III - 1
PCODE ST INT
WRPADIGETVTVP VIPS_RAT 61 10
WRPADVGETVTVP VIPS_MOUSE 61 10
WRPANVGETVTVP VIPS_HUMAN 62 10
VIP2RECEPTOR4 Length of motif = 14 Motif number = 4
Vasoactive intestinal polypeptide receptor 2 motif IV - 1
PCODE ST INT
KVFSNFYSRPGNIS VIPS_RAT 76 2
KVFSNFYSRPGNIS VIPS_MOUSE 76 2
KVFSNFYSKAGNIS VIPS_HUMAN 77 2
VIP2RECEPTOR5 Length of motif = 15 Motif number = 5
Vasoactive intestinal polypeptide receptor 2 motif V - 1
PCODE ST INT
EDESKITFYILVKAI VIPS_RAT 114 24
EDESKISFYILVKAI VIPS_MOUSE 114 24
EDESKITFYILVKAI VIPS_HUMAN 115 24
VIP2RECEPTOR6 Length of motif = 18 Motif number = 6
Vasoactive intestinal polypeptide receptor 2 motif VI - 1
PCODE ST INT
YSSSGTLRCHDQPGSWVG VIPS_RAT 183 54
YSSSGLLRCHDQPASWVG VIPS_MOUSE 183 54
YSSSGTLHCPDQPSSWVG VIPS_HUMAN 184 54
VIP2RECEPTOR7 Length of motif = 16 Motif number = 7
Vasoactive intestinal polypeptide receptor 2 motif VII - 1
PCODE ST INT
VAILPPSRCFLAYLLI VIPS_RAT 231 30
VAILPPSRCFLAYLLI VIPS_MOUSE 231 30
VAMLPPRRCFLAYLLI VIPS_HUMAN 232 30
VIP2RECEPTOR8 Length of motif = 12 Motif number = 8
Vasoactive intestinal polypeptide receptor 2 motif VIII - 1
PCODE ST INT
PIGISSTYQILF VIPS_RAT 347 100
PIGISSTYQILF VIPS_MOUSE 347 100
PISISSKYQILF VIPS_HUMAN 348 100
VIP2RECEPTOR9 Length of motif = 16 Motif number = 9
Vasoactive intestinal polypeptide receptor 2 motif IX - 1
PCODE ST INT
WRGLCLTQPGSRDYRL VIPS_RAT 389 30
WRGLCLTQAGSRDYRL VIPS_MOUSE 389 30
WRSRCPTPSASRDYRV VIPS_HUMAN 390 30
VIP2RECEPTOR10 Length of motif = 24 Motif number = 10
Vasoactive intestinal polypeptide receptor 2 motif X - 1
PCODE ST INT
SRNGSESALQIHRGSRTQSFLQSE VIPS_RAT 410 5
SRNGSESALQIHRGSRTQSFLQSE VIPS_MOUSE 410 5
SRNGSEGALQFHRGSRAQSFLQTE VIPS_HUMAN 411 5
User query: Display/Full Code "VIP2RECEPTOR"