WORKLIST ENTRIES (1):
XHISDIPTASE View alignment X-His dipeptidase (M25) signature
Type of fingerprint: COMPOUND with 9 elements
Links:
PRINTS; PR00756 ALADIPTASE; PR00791 PEPDIPTASEA; PR00730 THERMOLYSIN
PRINTS; PR00787 NEUTRALPTASE; PR00782 LSHMANOLYSIN; PR00931 MICOLLPTASE
PRINTS; PR00997 FRAGILYSIN; PR00786 NEPRILYSIN; PR00765 CRBOXYPTASEA
PRINTS; PR00932 AMINO1PTASE; PR00789 OSIALOPTASE; PR00933 BLYTICPTASE
PRINTS; PR00919 THERMOPTASE; PR00998 CRBOXYPTASET; PR00768 DEUTEROLYSIN
PRINTS; PR00999 FUNGALYSIN; PR01000 SREBPS2PTASE
INTERPRO; IPR001160
Creation date 09-SEP-1998; UPDATE 07-JUN-1999
1. RAWLINGS, N.D. AND BARRETT, A.J.
Evolutionary families of metallopeptidases.
METHODS ENZYMOL. 248 183-228 (1995).
2. RAWLINGS, N.D. AND BARRETT, A.J.
MEROPS - Peptidase Database
http://www.bi.bbsrc.ac.uk/merops/merops.htm
3. RAWLINGS, N.D. AND BARRETT, A.J.
Family M25 - Clan MH - X-His dipeptidase
http://www.bi.bbsrc.ac.uk/merops/famcards/m25.htm
4. HENRICH B., MONNERJAHN U., PLAPP R.
Peptidase D gene (pepD) of Escherichia coli K-12: nucleotide sequence,
transcript mapping, and comparison with othe peptidase genes.
J.BACTERIOL. 172 4641-4651 (1990).
Metalloproteases are the most diverse of the four main types of protease,
with more than 30 families identified to date [1]. Of these, around
half contain the HEXXH motif, which has been shown in crystallographic
studies to form part of the metal-binding site [1]. The HEXXH motif is
relatively common, but can be more stringently defined for metallo-
proteases as abXHEbbHbc, where a is most often valine or threonine and
forms part of the S1' subsite in thermolysin and neprilysin, b is an
uncharged residue, and c a hydrophobic residue. Proline is never found
in this site, possibly because it would break the helical structure
adopted by this motif in metalloproteases [1].
Metalloproteases can be split into five groups on the basis of their metal-
binding residues: the first three contain the HEXXH motif, the other two
do not [1]. In the first group, a glutamic acid completes the active site -
these are termed HEXXH+E: all families in this group show some sequence
relationship and have been assigned to clan MA [1]. The second group, which
have a third histidine as the extra metal-binding residue, are termed
HEXXH+H and are grouped into clan MB on the basis of their inter-relation-
ship[1]. In the third group, the additional metal-binding residues are
unidentified. The fourth group is diverse - the metal-binding residues are
known but do not form the HEXXH motif. And the fifth group comprises the
remaining families where the metal-binding residues are as yet unknown [1,2].
X-His dipeptidases are zinc-containing metallopeptidases that belong to the
M25 protease family, which forms part of the MH clan [1,3]. These cyto-
plasmic endopeptidases cleave Xaa+His dipeptides (where Xaa is any hydro-
phobic residue). The zinc ligands of the MH clan are His/Asp, Asp, Glu,
Asp/Glu and His [1,2].
The amino acid sequence deduced from E.coli reveals that peptidase D is a
slightly hydrophilic protein of 485 residues that contains no extended
domains of marked hydrophobicity [4].
XHISDIPTASE is a 9-element fingerprint that provides a signature for X-His
metallodipeptidases (M25). The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length. A single iteration on OWL30.2
was required to reach convergence, no further sequences being identified
beyond the starting set.
An update on SPTR37_9f identified a true set of 3 sequences.
SUMMARY INFORMATION
3 codes involving 9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
COMPOSITE FINGERPRINT INDEX
9| 3 3 3 3 3 3 3 3 3
8| 0 0 0 0 0 0 0 0 0
7| 0 0 0 0 0 0 0 0 0
6| 0 0 0 0 0 0 0 0 0
5| 0 0 0 0 0 0 0 0 0
4| 0 0 0 0 0 0 0 0 0
3| 0 0 0 0 0 0 0 0 0
2| 0 0 0 0 0 0 0 0 0
--+----------------------------------------------
| 1 2 3 4 5 6 7 8 9
True positives..
PEPD_ECOLI PEPD_HAEIN O51553
PROTEIN TITLES
PEPD_ECOLI AMINOACYL-HISTIDINE DIPEPTIDASE (EC 3.4.13.3) (XAA-HIS DIPEP
PEPD_HAEIN AMINOACYL-HISTIDINE DIPEPTIDASE (EC 3.4.13.3) (XAA-HIS DIPEP
O51553 AMINOACYL-HISTIDINE DIPEPTIDASE (PEPD) - BORRELIA BURGDORFER
SCAN HISTORY
OWL30_2 2 50 NSINGLE
SPTR37_9f 2 4 NSINGLE
INITIAL MOTIF SETS
XHISDIPTASE1 Length of motif = 20 Motif number = 1
X-His metallodipeptidase motif I - 1
PCODE ST INT
VPQKNNDTVHDFTKDPIQPY PEPD_ECOLI 79 79
VPQANEGNPHDFTKDPIQPY PEPD_HAEIN 79 79
VCEKNESSLHNFETDPIEIV G70175 71 71
XHISDIPTASE2 Length of motif = 19 Motif number = 2
X-His metallodipeptidase motif II - 1
PCODE ST INT
DGEWVKARGTTLGADNGIG PEPD_ECOLI 100 1
DGEWVKARGTTLGSDNGIG PEPD_HAEIN 100 1
EDGYLKAVGTTLGADNGIG G70175 92 1
XHISDIPTASE3 Length of motif = 18 Motif number = 3
X-His metallodipeptidase motif III - 1
PCODE ST INT
GPLEVLLTMTEEAGMDGA PEPD_ECOLI 134 15
PPLEVLLTMTEETGMDGA PEPD_HAEIN 134 15
PDLELLFTVDEEIGLIGA G70175 127 16
XHISDIPTASE4 Length of motif = 19 Motif number = 4
X-His metallodipeptidase motif IV - 1
PCODE ST INT
WLQADILINTDSEEEGEIY PEPD_ECOLI 158 6
WLQSEILINTDTEEIGEIY PEPD_HAEIN 158 6
LCSGKSLINLDGEEEGYFL G70175 151 6
XHISDIPTASE5 Length of motif = 18 Motif number = 5
X-His metallodipeptidase motif V - 1
PCODE ST INT
LTLKGLKGGHSGGEIHVG PEPD_ECOLI 205 28
ITLKGLRGGHSGGDIHTG PEPD_HAEIN 204 27
ILFKGLKGGHSGADIHLD G70175 197 27
XHISDIPTASE6 Length of motif = 20 Motif number = 6
X-His metallodipeptidase motif VI - 1
PCODE ST INT
GGTLRNAIPREAFATIAVAA PEPD_ECOLI 250 27
GGSIRNAIPREAAAVLAFNG PEPD_HAEIN 250 28
GGNSSNAIPIEAKALIFIDD G70175 242 27
XHISDIPTASE7 Length of motif = 19 Motif number = 7
X-His metallodipeptidase motif VII - 1
PCODE ST INT
LIRSLIDSGKDYVVSMLDS PEPD_ECOLI 362 92
LVRSLIESGKYYVTEMLSS PEPD_HAEIN 361 91
LIRSLLDLDKEYVCNHLQS G70175 354 92
XHISDIPTASE8 Length of motif = 17 Motif number = 8
X-His metallodipeptidase motif VIII - 1
PCODE ST INT
IHAGLECGLFKKPYPEM PEPD_ECOLI 427 46
IHAGLECGLLKEHYPNI PEPD_HAEIN 426 46
IHAGLETGIISSRLGGI G70175 419 46
XHISDIPTASE9 Length of motif = 23 Motif number = 9
X-His metallodipeptidase motif IX - 1
PCODE ST INT
GPTITGPHSPDEQVHIESVGHYW PEPD_ECOLI 449 5
GPTIRNAHSPDEKVEIATVQTYW PEPD_HAEIN 448 5
GPWIEWPHTTRERVNISSTIRVY G70175 441 5
FINAL MOTIF SETS
XHISDIPTASE1 Length of motif = 20 Motif number = 1
X-His metallodipeptidase motif I - 2
PCODE ST INT
VPQKNNDTVHDFTKDPIQPY PEPD_ECOLI 79 79
VPQANEGNPHDFTKDPIQPY PEPD_HAEIN 79 79
VCEKNESSLHNFETDPIEIV O51553 71 71
XHISDIPTASE2 Length of motif = 19 Motif number = 2
X-His metallodipeptidase motif II - 2
PCODE ST INT
DGEWVKARGTTLGADNGIG PEPD_ECOLI 100 1
DGEWVKARGTTLGSDNGIG PEPD_HAEIN 100 1
EDGYLKAVGTTLGADNGIG O51553 92 1
XHISDIPTASE3 Length of motif = 18 Motif number = 3
X-His metallodipeptidase motif III - 2
PCODE ST INT
GPLEVLLTMTEEAGMDGA PEPD_ECOLI 134 15
PPLEVLLTMTEETGMDGA PEPD_HAEIN 134 15
PDLELLFTVDEEIGLIGA O51553 127 16
XHISDIPTASE4 Length of motif = 19 Motif number = 4
X-His metallodipeptidase motif IV - 2
PCODE ST INT
WLQADILINTDSEEEGEIY PEPD_ECOLI 158 6
WLQSEILINTDTEEIGEIY PEPD_HAEIN 158 6
LCSGKSLINLDGEEEGYFL O51553 151 6
XHISDIPTASE5 Length of motif = 18 Motif number = 5
X-His metallodipeptidase motif V - 2
PCODE ST INT
LTLKGLKGGHSGGEIHVG PEPD_ECOLI 205 28
ITLKGLRGGHSGGDIHTG PEPD_HAEIN 204 27
ILFKGLKGGHSGADIHLD O51553 197 27
XHISDIPTASE6 Length of motif = 20 Motif number = 6
X-His metallodipeptidase motif VI - 2
PCODE ST INT
GGTLRNAIPREAFATIAVAA PEPD_ECOLI 250 27
GGSIRNAIPREAAAVLAFNG PEPD_HAEIN 250 28
GGNSSNAIPIEAKALIFIDD O51553 242 27
XHISDIPTASE7 Length of motif = 19 Motif number = 7
X-His metallodipeptidase motif VII - 2
PCODE ST INT
LIRSLIDSGKDYVVSMLDS PEPD_ECOLI 362 92
LVRSLIESGKYYVTEMLSS PEPD_HAEIN 361 91
LIRSLLDLDKEYVCNHLQS O51553 354 92
XHISDIPTASE8 Length of motif = 17 Motif number = 8
X-His metallodipeptidase motif VIII - 2
PCODE ST INT
IHAGLECGLFKKPYPEM PEPD_ECOLI 427 46
IHAGLECGLLKEHYPNI PEPD_HAEIN 426 46
IHAGLETGIISSRLGGI O51553 419 46
XHISDIPTASE9 Length of motif = 23 Motif number = 9
X-His metallodipeptidase motif IX - 2
PCODE ST INT
GPTITGPHSPDEQVHIESVGHYW PEPD_ECOLI 449 5
GPTIRNAHSPDEKVEIATVQTYW PEPD_HAEIN 448 5
GPWIEWPHTTRERVNISSTIRVY O51553 441 5
User query: Display/Full Code "XHISDIPTASE"